Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
|Tested species reactivity||Human , Mouse|
|Published species reactivity||Ferret , Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the C-terminal region of the human and mouse amyloid-beta precursor protein (APP695)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg x 10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||2 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer"e;s disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE). This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase, furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. Gamma-secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. Found associated with the presenilins is the transmembrane glycoprotein nicastrin. Nicastrin has been found to bind to the carboxyl-terminus of betaAPP and helps to modulate the production of the amyloid beta peptide.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Reduced subventricular zone proliferation and white matter damage in juvenile ferrets with kaolin-induced hydrocephalus.
36-6900 was used in immunohistochemistry to characterize the behavioral, structural, and histological changes in juvenile ferrets following induction of hydrocephalus.
|Di Curzio DL,Buist RJ,Del Bigio MR||Experimental neurology (248:112)||2013|
Critical role of NADPH oxidase in neuronal oxidative damage and microglia activation following traumatic brain injury.
36-6900 was used in western blot to elucidate the role of NADPH oxidase in traumatic brain injury.
|Zhang QG,Laird MD,Han D,Nguyen K,Scott E,Dong Y,Dhandapani KM,Brann DW||PloS one (7:null)||2012|
Increased AβPP processing in familial Danish dementia patients.
36-6900 was used in western blot to test if AβPP processing is altered in Familial Danish Dementia patients' brain samples.
|Matsuda S,Tamayev R,D'Adamio L||Journal of Alzheimer's disease : JAD (27:385)||2011|
APP heterozygosity averts memory deficit in knockin mice expressing the Danish dementia BRI2 mutant.
36-6900 was used in western blot to study APP/Bri2 complexes in a mouse model of familial Danish dementia.
|Tamayev R,Matsuda S,Giliberto L,Arancio O,D'Adamio L||The EMBO journal (30:2501)||2011|
The familial dementia BRI2 gene binds the Alzheimer gene amyloid-beta precursor protein and inhibits amyloid-beta production.
||Matsuda S,Giliberto L,Matsuda Y,Davies P,McGowan E,Pickford F,Ghiso J,Frangione B,D'Adamio L||The Journal of biological chemistry (280:28912)||2005|
PN-II, CVAP, E030013M08Rik, ABETA, ABPP, CTFgamma, PN2, Ag, AAA, Abeta, betaApp, Abpp, APPI, Adap, Cvap, AD1
alzheimer disease amyloid protein, amyloid beta A4 protein, beta-amyloid peptide, cerebral vascular amyloid peptide, peptidase nexin-II, preA4, protease nexin-II, alzheimer disease amyloid A4 protein homolog, amyloidogenic glycoprotein, appican, protease nexin II, AAA, ABETA, AD1, CTFgamma, CVAP, PN2, APP, Amyloidogenic glycoprotein AG, Amyloid beta A4 protein, amyloid beta (A4) precursor protein, Peptidase nexin-II