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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • beta-Arrestin 2 Antibodies

          Invitrogen

          beta-Arrestin 2 Polyclonal Antibody

          Advanced Verification
          5 Published Figures
          7 References
          View all (15) beta-Arrestin 2 antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite beta-Arrestin 2 Polyclonal Antibody

          • Antibody Testing Data (2)
          • Published Figures (5)
          • Advanced Verification (1)
          beta-Arrestin 2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          beta-Arrestin 2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 8

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          beta-Arrestin 2 Antibody (PA1-732) in ICC/IF

          Immunofluorescence analysis of beta-Arrestin 2 was performed using 70% confluent log phase HEK293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with beta-Arrestin 2 Rabbit Polyclonal Antibody (Product # PA1-732) at 2µg/mL in 0.1% BSA and incubated for 3 ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          beta-Arrestin 2 Antibody in Immunocytochemistry (ICC/IF)
          beta-Arrestin 2 Antibody in Western Blot (WB)
          beta-Arrestin 2 Antibody in Western Blot (WB)
          beta-Arrestin 2 Antibody in Western Blot (WB)
          beta-Arrestin 2 Antibody in Western Blot (WB)
          beta-Arrestin 2 Antibody in Western Blot (WB)
          beta-Arrestin 2 Antibody in Immunoprecipitation (IP)
          beta-Arrestin 2 Antibody

          Product Details

          PA1-732

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000
          View 5 publications 5 publications

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          2 µg/mL
          -

          Immunoprecipitation (IP)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Bovine, Human, Mouse, Rat

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide corresponding to residues C D(384) D I V F E D F A R L R L K(397) of human beta-arrestin 2.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 1mg/mL BSA

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2060256

          Product Specific Information

          PA1-732 detects beta-Arrestin 2 in rat, human and bovine samples.

          PA1-732 has been successfully used in Western blot procedures. By Western blot, this antibody detects ~49 kDa and ~47 kDa proteins representing pan Arrestin in rat and bovine retina as well as rat brain samples.

          The PA1-732 immunogen is a synthetic peptide corresponding to residues C D(384) D I V F E D F A R L R L K(397) of human beta-arrestin 2. This peptide (Cat. # PEP-281) is available for use in neutralization and control experiments.

          Target Information

          Beta-arrestin-2, or ARRB2, is an adaptor protein belonging to the arrestin family with an N- arrestin domain and a C- arrestin domain. ARRB2 is a regulatory protein involved in heterotrimeric G protein-coupled receptor desensitization and is known to regulate beta2-adrenergic receptor (beta2AR) function by enhancing beta2AR mediated nuclear translocation of ERK. ARRB2 binds to phosphorylated beta2ARs, thereby causing a significant impairment of their capacity to activate G(S) proteins. Along with AIP4, ARRB2 acts as an endosomal sorting molecule that mediates CXCR4 entry into a degradative pathway. It may also be involved in hormone-specific desensitization of TSH receptors. ARRB2 is a Ca2+ binding protein of the retinal outer segments and binds to P-rhodopsin and is also involved in synaptic transmission in photoreceptor cells. It is widely expressed in most of the tissues, especially neuronal tissues and spleen.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: arrestin 3; Arrestin beta-2; arrestin, beta 2; arrestin-3; beta arr2; beta-arrestin 2 long isoform; beta-arrestin 2 short isoform; Beta-arrestin-2; Non-visual arrestin-3

          View more View less

          Gene Aliases: AI326910; ARB2; ARR2; ARRB2; AW122872; BARR2; BARRES

          View more View less

          UniProt ID: (Human) P32121, (Mouse) Q91YI4, (Rat) P29067

          View more View less

          Entrez Gene ID: (Bovine) 281638, (Human) 409, (Mouse) 216869, (Rat) 25388

          View more View less

          Function(s)
          inositol hexakisphosphate binding phosphatidylinositol-3,4,5-trisphosphate binding phosphatidylinositol binding G-protein coupled receptor binding receptor binding protein binding enzyme binding protein domain specific binding ubiquitin protein ligase binding alpha-1A adrenergic receptor binding alpha-1B adrenergic receptor binding angiotensin receptor binding type 1 angiotensin receptor binding D1 dopamine receptor binding follicle-stimulating hormone receptor binding type 2A serotonin receptor binding platelet activating factor receptor binding protein complex binding protein complex scaffold protein kinase B binding mitogen-activated protein kinase binding 14-3-3 protein binding clathrin binding scaffold/adaptor protein
          Process(es)
          desensitization of G-protein coupled receptor protein signaling pathway G-protein coupled receptor internalization positive regulation of receptor internalization signal transduction protein transport receptor internalization desensitization of G-protein coupled receptor protein signaling pathway by arrestin transcription from RNA polymerase II promoter transforming growth factor beta receptor signaling pathway dopamine receptor signaling pathway brain development adult walking behavior protein ubiquitination platelet activation negative regulation of protein ubiquitination positive regulation of protein ubiquitination negative regulation of NF-kappaB transcription factor activity positive regulation of synaptic transmission, dopaminergic negative regulation of interleukin-1 beta production negative regulation of interleukin-12 production negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production positive regulation of peptidyl-serine phosphorylation negative regulation of toll-like receptor signaling pathway negative regulation of GTPase activity negative regulation of smooth muscle cell apoptotic process follicle-stimulating hormone signaling pathway negative regulation of cysteine-type endopeptidase activity involved in apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of natural killer cell mediated cytotoxicity positive regulation of peptidyl-tyrosine phosphorylation detection of temperature stimulus involved in sensory perception of pain positive regulation of protein kinase B signaling negative regulation of protein kinase B signaling positive regulation of calcium ion transport Wnt signaling pathway, planar cell polarity pathway cell chemotaxis regulation of androgen receptor signaling pathway positive regulation of ERK1 and ERK2 cascade negative regulation of release of cytochrome c from mitochondria chemical synaptic transmission, postsynaptic positive regulation of DNA biosynthetic process regulation of protein phosphorylation positive regulation of protein phosphorylation transport endocytosis G-protein coupled receptor signaling pathway regulation of G-protein coupled receptor protein signaling pathway negative regulation of signal transduction regulation of cAMP biosynthetic process regulation of cAMP catabolic process
          It has to be done as per old AB suggested Products section.
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