Invitrogen

beta-Arrestin 2 Polyclonal Antibody

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5 Published Figures
7 References
View all (15) beta-Arrestin 2 antibodies
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Datasheet
Protocols
Questions & Answers

Product Details

PA1-732

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000
View 5 publications 5 publications

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL
-

Immunoprecipitation (IP)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C D(384) D I V F E D F A R L R L K(397) of human beta-arrestin 2.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2060256

Product Specific Information

PA1-732 detects beta-Arrestin 2 in rat, human and bovine samples.

PA1-732 has been successfully used in Western blot procedures. By Western blot, this antibody detects ~49 kDa and ~47 kDa proteins representing pan Arrestin in rat and bovine retina as well as rat brain samples.

The PA1-732 immunogen is a synthetic peptide corresponding to residues C D(384) D I V F E D F A R L R L K(397) of human beta-arrestin 2. This peptide (Cat. # PEP-281) is available for use in neutralization and control experiments.

Target Information

Beta-arrestin-2, or ARRB2, is an adaptor protein belonging to the arrestin family with an N- arrestin domain and a C- arrestin domain. ARRB2 is a regulatory protein involved in heterotrimeric G protein-coupled receptor desensitization and is known to regulate beta2-adrenergic receptor (beta2AR) function by enhancing beta2AR mediated nuclear translocation of ERK. ARRB2 binds to phosphorylated beta2ARs, thereby causing a significant impairment of their capacity to activate G(S) proteins. Along with AIP4, ARRB2 acts as an endosomal sorting molecule that mediates CXCR4 entry into a degradative pathway. It may also be involved in hormone-specific desensitization of TSH receptors. ARRB2 is a Ca2+ binding protein of the retinal outer segments and binds to P-rhodopsin and is also involved in synaptic transmission in photoreceptor cells. It is widely expressed in most of the tissues, especially neuronal tissues and spleen.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: arrestin 3; Arrestin beta-2; arrestin, beta 2; arrestin-3; beta arr2; beta-arrestin 2 long isoform; beta-arrestin 2 short isoform; Beta-arrestin-2; Non-visual arrestin-3

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Gene Aliases: AI326910; ARB2; ARR2; ARRB2; AW122872; BARR2; BARRES

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UniProt ID: (Human) P32121, (Mouse) Q91YI4, (Rat) P29067

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Entrez Gene ID: (Bovine) 281638, (Human) 409, (Mouse) 216869, (Rat) 25388

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Function(s)
inositol hexakisphosphate binding phosphatidylinositol-3,4,5-trisphosphate binding phosphatidylinositol binding G-protein coupled receptor binding receptor binding protein binding enzyme binding protein domain specific binding ubiquitin protein ligase binding alpha-1A adrenergic receptor binding alpha-1B adrenergic receptor binding angiotensin receptor binding type 1 angiotensin receptor binding D1 dopamine receptor binding follicle-stimulating hormone receptor binding type 2A serotonin receptor binding platelet activating factor receptor binding protein complex binding protein complex scaffold protein kinase B binding mitogen-activated protein kinase binding 14-3-3 protein binding clathrin binding scaffold/adaptor protein
Process(es)
desensitization of G-protein coupled receptor protein signaling pathway G-protein coupled receptor internalization positive regulation of receptor internalization signal transduction protein transport receptor internalization desensitization of G-protein coupled receptor protein signaling pathway by arrestin transcription from RNA polymerase II promoter transforming growth factor beta receptor signaling pathway dopamine receptor signaling pathway brain development adult walking behavior protein ubiquitination platelet activation negative regulation of protein ubiquitination positive regulation of protein ubiquitination negative regulation of NF-kappaB transcription factor activity positive regulation of synaptic transmission, dopaminergic negative regulation of interleukin-1 beta production negative regulation of interleukin-12 production negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production positive regulation of peptidyl-serine phosphorylation negative regulation of toll-like receptor signaling pathway negative regulation of GTPase activity negative regulation of smooth muscle cell apoptotic process follicle-stimulating hormone signaling pathway negative regulation of cysteine-type endopeptidase activity involved in apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of natural killer cell mediated cytotoxicity positive regulation of peptidyl-tyrosine phosphorylation detection of temperature stimulus involved in sensory perception of pain positive regulation of protein kinase B signaling negative regulation of protein kinase B signaling positive regulation of calcium ion transport Wnt signaling pathway, planar cell polarity pathway cell chemotaxis regulation of androgen receptor signaling pathway positive regulation of ERK1 and ERK2 cascade negative regulation of release of cytochrome c from mitochondria chemical synaptic transmission, postsynaptic positive regulation of DNA biosynthetic process regulation of protein phosphorylation positive regulation of protein phosphorylation transport endocytosis G-protein coupled receptor signaling pathway regulation of G-protein coupled receptor protein signaling pathway negative regulation of signal transduction regulation of cAMP biosynthetic process regulation of cAMP catabolic process
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