|Tested species reactivity||Bovine, Human, Rat|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C D(384) D I V F E D F A R L R L K(397) of human beta-arrestin 2.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-732 detects beta-Arrestin 2 in rat, human and bovine samples.
PA1-732 has been successfully used in Western blot procedures. By Western blot, this antibody detects ~49 kDa and ~47 kDa proteins representing pan Arrestin in rat and bovine retina as well as rat brain samples.
The PA1-732 immunogen is a synthetic peptide corresponding to residues C D(384) D I V F E D F A R L R L K(397) of human beta-arrestin 2. This peptide (Cat. # PEP-281) is available for use in neutralization and control experiments.
Beta-arrestin-2, or ARRB2, is an adaptor protein belonging to the arrestin family with an N- arrestin domain and a C- arrestin domain. ARRB2 is a regulatory protein involved in heterotrimeric G protein-coupled receptor desensitization and is known to regulate beta2-adrenergic receptor (beta2AR) function by enhancing beta2AR mediated nuclear translocation of ERK. ARRB2 binds to phosphorylated beta2ARs, thereby causing a significant impairment of their capacity to activate G(S) proteins. Along with AIP4, ARRB2 acts as an endosomal sorting molecule that mediates CXCR4 entry into a degradative pathway. It may also be involved in hormone-specific desensitization of TSH receptors. ARRB2 is a Ca2+ binding protein of the retinal outer segments and binds to P-rhodopsin and is also involved in synaptic transmission in photoreceptor cells. It is widely expressed in most of the tissues, especially neuronal tissues and spleen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Arrestin competition influences the kinetics and variability of the single-photon responses of mammalian rod photoreceptors.
PA1-732 was used in western blot to study the effect of GRK1 and arrestin 1 on the kinetics and variability of single-photon responses of a rod.
|Doan T,Azevedo AW,Hurley JB,Rieke F||The Journal of neuroscience : the official journal of the Society for Neuroscience (29:11867)||2009|
Dopamine promotes striatal neuronal apoptotic death via ERK signaling cascades.
PA1-732 was used in immunoprecipitation to investigate the role of D1 receptor-mediated ERK activation in striatal neurodegeneration
|Chen J,Rusnak M,Lombroso PJ,Sidhu A||The European journal of neuroscience (29:287)||2009|