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|Tested species reactivity||Bacteria|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Storage buffer||PBS, pH 7.2|
|Contains||5mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 5 publications below|
Beta Galactosidase is an exoglycosidase which hydrolyzes the beta-glycosidic bond formed between a galactose and its organic moiety. Deficiencies in the protein in humans can result in galactosialidosis or Morquio B syndrome. In E. coli, the gene of beta-galactosidase, the lacZ gene, is present as part of the inducible system lac operon which is activated in the presence of lactose when glucose level is low. E. coli beta Galactosidase is commonly used in molecular biology as a reporter marker to monitor gene expression. Another popular use for beta Galactosidase is in blue/white screening to identify recombinant clones. Beta Galactosidase can be split in two peptides, lacZalpha and LacZOmega, neither of which is active by itself but when both are present together, spontaneously reassemble into a functional enzyme. This property is exploited in many cloning vectors. The presence or absence of an active beta Galactosidase may be detected through addition of artificial chromogenic substrates such as X-gal, fluorescent substrates such as Fluorescein di-beta-D-galactopyranoside (FDG), luminescent substrates and others. Beta Galactosidase activity at pH 6 is an indicator of senescent cells not found in presenescent, quiescent or dividing cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Characterization of Panglial Gap Junction Networks in the Thalamus, Neocortex, and Hippocampus Reveals a Unique Population of Glial Cells.
A-11132 was used in immunohistochemistry to describe the formation of coupled networks between astrocytes and oligodendrocytes in the murine ventrobasal thalamus and compare these associations with those found in the hippocampus and cortex.
|Griemsmann S,Höft SP,Bedner P,Zhang J,von Staden E,Beinhauer A,Degen J,Dublin P,Cope DW,Richter N,Crunelli V,Jabs R,Willecke K,Theis M,Seifert G,Kettenmann H,Steinhäuser C||Cerebral cortex (New York, N.Y. : 1991) (25:3420)||2015|
Progressive dysfunction of the retinal pigment epithelium and retina due to increased VEGF-A levels.
A-11132 was used in immunohistochemistry to evaluate the effect of increased VEGF-A levels on the retinal pigment epithelium and retina.
|Ablonczy Z,Dahrouj M,Marneros AG||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (28:2369)||2014|
Macrophages are essential for the early wound healing response and the formation of a fibrovascular scar.
A-11132 was used in immunohistochemistry to identify the role of macrophages in wound healing and fibrovascular scar production.
|He L,Marneros AG||The American journal of pathology (182:2407)||2013|
Wnt/β-catenin signaling is disrupted in the extra-toes (Gli3(Xt/Xt) ) mutant from early stages of forebrain development, concomitant with anterior neural plate patterning defects.
A-11132 was used in immunohistochemistry to study the role of Gli3 during forebrain development in mice
|Fotaki V,Price DJ,Mason JO||The Journal of comparative neurology (519:1640)||2011|
Chemokine receptor expression by neural progenitor cells in neurogenic regions of mouse brain.
A-11132 was used in immunohistochemistry to study the involvement of chemokine receptor in neurogenesis
|Tran PB,Banisadr G,Ren D,Chenn A,Miller RJ||The Journal of comparative neurology (500:1007)||2007|