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Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
Homologous proteins of different species frequently share antigenic determinants. Cross-reactivity has been observed with milk of Sheep and Goat.
In immunoelectrophoresis use 2 µL serum, plasma or equivalent against 120 µL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 µL antiserum in a 3 mm diameter centre well and 2 µL serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single radial immunodiffusion and electroimmunodiffusion use 0.5 to 1.0 percent antiserum in the agar gel.
Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4°C, not refrozen, and preferably used the same day.
Beta-lactoglobulin is a major protein found in dairy products and is not present in human milk. It has been identified as a potential milk allergen, and is responsible for transport of both iron and hydrophobic molecules.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: beta lactoglobulin C; beta lactoglobulin D; Beta-lactoglobulin; Beta-LG; bLG C; lactoglobulin, beta
Gene Aliases: BLG; LGB
UniProt ID: (Bovine) P02754
Entrez Gene ID: (Bovine) 280838
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