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|Tested species reactivity||Bovine, Chicken, Hamster, Human, Mouse, Primate, Pig, Rat, Clawed frog, Zebrafish|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide between amino acids 1-100 of the human Beta-Tubulin protein.|
|Purification||Ammonium sulfate precipitation|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:1000-1:2000|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
Suggested positive control: antigen standard for TUBB (transient overexpression lysate), Hela whole cell extract.
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton. Antibodies against beta Tubulin are useful as loading controls for Western Blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The Role of Sonic Hedgehog in the Specification of Human Cortical Progenitors In Vitro.
PA1-16947 was used in western blot to study the relation between human cortical formation and impaired sonic hedgehog signaling
|Radonjić NV,Memi F,Ortega JA,Glidden N,Zhan H,Zecevic N||Cerebral cortex (New York, N.Y. : 1991) (26:131)||2016|
The new 4-O-methylhonokiol analog GS12021 inhibits inflammation and macrophage chemotaxis: role of AMP-activated protein kinase α activation.
PA1-16947 was used in western blot to determine if methylhonokiol analogs inhibit the expression of inflammatory genes in macrophages and adipocytes
|Kim S,Ka SO,Lee Y,Park BH,Fei X,Jung JK,Seo SY,Bae EJ||PloS one (10:null)||2015|
Inhibition of adipocyte inflammation and macrophage chemotaxis by butein.
PA1-16947 was used in western blot to study the mechanisms underlying the inhibitory effects of butein on adipocyte inflammation and macrophage chemotaxis
|Wang Z,Lee Y,Eun JS,Bae EJ||European journal of pharmacology (738:40)||2014|
beta 5-tubulin; beta Ib tubulin; beta5-tubulin; c(beta)7 tubulin; M40 antibody; tubb beta-5; tubulin beta 4'; tubulin beta chain; Tubulin beta polypeptide antibody.; tubulin beta-1; tubulin beta-1 chain; tubulin beta-5 chain; tubulin beta-7 chain; tubulin, beta 1 class VI; tubulin, beta 2A; tubulin, beta 5; tubulin, beta class I; tubulin, beta family 1; tubulin, beta polypeptide
2810484G07Rik; CDCBM6; CH242-247L10.4; CSCSC1; M(beta)1; M40; OK/SW-cl.56; TUBB; TUBB1; TUBB2A; TUBB5; XLOT