Proteintech

c-Cbl Polyclonal Antibody

c-Cbl Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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c-Cbl Antibody (25818-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using (Product # 25818-1-AP) (c-Cbl antibody) at dilution of 1:1,000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

c-Cbl Antibody in Immunoprecipitation (IP)

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Product Details

25818-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:50,000

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

Immunocytochemistry (ICC/IF)

Assay-dependent

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

c-Cbl Fusion Protein Ag23033 (578-906 aa encoded by BC132733)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: LPPVPSSRL GDSWLPRPIP KVPVSAPSSS DPWTGRELTN RHSLPFSLPS QMEPRPDVPR LGSTFSLDTS MSMNSSPLVG PECDHPKIKP SSSANAIYSL AARPLPVPKL PPGEQCEGEE DTEYMTPSSR PLRPLDTSQS SRACDCDQQI DSCTYEAMYN IQSQAPSITE SSTFGEGNLA AAHANTGPEE SENEDDGYDV PKPPVPAVLA RRTLSDISNA SSSFGWLSLD GDPTTNVTEG SQVPERPPKP FPRRINSERK AGSCQQGSGP AASAATASPQ LSSEIENLMS QGYSYQDIQK ALVIAQNNIE MAKNILREFV SISSPAHVAT (578-906 aa encoded by BC132733)

Target Information

Cbl proteins are a family of ubiquitin protein ligases (E3s) that negatively regulate signaling by targeting activated tyrosine kinases for degradation. Cbl- c is the most recently cloned member of the Cbl proteins and is expressed only in epithelial cells (the other Cbl proteins are ubiquitously expressed). Cbl-c, like the other mammalian Cbl proteins, can ubiquitinate the activated EGFR and target it for degradation. Through interactions with proteins containing SRC homology-2 (SH2) and SH3 domains, CBL proteins modulate downstream cell signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Hepatology (Baltimore, Md.)

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Hepatology (Baltimore, Md.)

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Thu Nov 01 00:00:00 EDT 2018

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Hepatology (Baltimore, Md.)

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Bioinformatics

Protein Aliases: Cas-Br-M (murine) ecotropic retroviral transforming sequence; Casitas B-lineage lymphoma proto-oncogene; Cbl proto-oncogene, E3 ubiquitin protein ligase; CBL-3; CBLC; E3 ubiquitin-protein ligase CBL; fragile site, folic acid type, rare, fra(11)(q23.3); oncogene CBL2; Proto-oncogene c-Cbl; RING finger protein 55; RING-type E3 ubiquitin transferase CBL; RNF57; Signal transduction protein CBL

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Gene Aliases: 4732447J05Rik; C-CBL; CBL; Cbl-2; CBL2; FRA11B; NSLL; RNF55

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UniProt ID: (Human) P22681, (Mouse) P22682

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Entrez Gene ID: (Human) 867, (Mouse) 12402

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Function(s)

phosphotyrosine binding transcription factor activity, sequence-specific DNA binding ubiquitin-protein transferase activity signal transducer activity calcium ion binding protein binding zinc ion binding ligase activity SH3 domain binding protein kinase binding ephrin receptor binding ubiquitin protein ligase activity cadherin binding involved in cell-cell adhesion UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity ligase metabolite interconversion enzyme

Process(es)

regulation of transcription, DNA-templated cell surface receptor signaling pathway epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway fibroblast growth factor receptor signaling pathway positive regulation of phosphatidylinositol 3-kinase signaling protein ubiquitination negative regulation of epidermal growth factor receptor signaling pathway protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of apoptotic process positive regulation of epidermal growth factor receptor signaling pathway positive regulation of receptor-mediated endocytosis cell-cell adhesion regulation of signaling

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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c-Cbl Polyclonal Antibody

c-Cbl Antibody (25818-1-AP) in IHC (P)

Product Details

25818-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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