|Tested species reactivity||Hamster, Rabbit, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Sheep / IgG|
|Immunogen||Synthetic peptide corresponding to the N-terminal residues M(2) F S G F N A D Y E A S S S R C(17).|
|Purification||Ammonium sulfate precipitation|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||10 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/mL|
|Western Blot (WB)||10 µ/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA1-18329 detects c-FOS from rat, rabbit, and hamster samples.
PA1-18329 has been successfully used in immunohistochemistry (paraffin and frozen) and Western blot procedures.
The PA1-18329 immunogen is a synthetic peptide corresponding to the N-terminal residues M(2) F S G F N A D Y E A S S S R C(17), conjugated to diphtheria toxoid. The sequence is 100% conserved in human, rat, mouse, hamster, and cat.
Reconstitute with 500 ul of distilled water.
c-FOS is a nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. It has a critical function in regulating the development of cells destined to form and maintain the skeleton, and is thought to have an important role in signal transduction, cell proliferation and differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Distribution of Fos-Like Immunoreactivity, Catecholaminergic and Serotoninergic Neurons Activated by the Laryngeal Chemoreflex in the Medulla Oblongata of Rats.
PA1-18329 was used in immunohistochemistry to assess the distribution of medullary nuclei that are activated by the laryngeal chemoreflex.
|Wang X,Guo R,Zhao W||PloS one (10:null)||2015|