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Invitrogen
c-Fos Polyclonal Antibody
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FIGURE: 1 / 4
c-Fos Antibody (PA1-830) in ICC/IF
Immunofluorescent analysis of c-FOS (green) in untreated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product # 31873) for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing c-FOS (Product # PA1-830), at a dilution of 1:500 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit secondary antibody at a dilution of ... View More
Product Details
PA1-830
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues S(4) G F N A D Y E A S S S R C(17) of human cFos.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Amount
200 µg
Purification
Ammonium sulfate precipitation
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-830 detects cFos from mouse and human cells.
PA1-830 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~60 kDa protein representing cFos in NIH 3T3 serum stimulated cells.
The PA1-830 immunogen is a synthetic peptide corresponding to residues S(4) G F N A D Y E A S S S R C(17) of human cFos. This sequence is completely conserved in mouse cFos.
Reconstitute with 200 µL of PBS (1 mg/mL).
Target Information
FBJ murine osteosarcoma viral oncogene homolog is commonly known as Cellular protooncogene Fos (c-Fos). It belongs to the fos transcription factor family and is encoded by the C-FOS gene located on chromosome 14. c-Fos heterodimerizes with c-Jun to form the Activator Protein -1 (AP-1) transcription factor which regulates transcription of several genes that play an important role in cellular signal transduction, cell proliferation and differentiation. c-Fos has been shown to be phosphorylated by various stimuli including growth factors and insulin at atleast seven different sites. The best known upstream kinase for phosphorylation at Threonine 232 is ERK MAPK which regulates localization of c-Fos to the nucleus and is thus important for c-Fos induced transcriptional activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: activator protein 1; cellular oncogene c-fos; Cellular oncogene fos; FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS); FBJ murine osteosarcoma viral oncogene homolog; FBJ osteosarcoma oncogene; FOS; Fos proto-oncogene, AP-1 trancription factor subunit; Fos proto-oncogene, AP-1 transcription factor subunit; G0/G1 switch regulatory protein 7; Protein c-Fos; Proto-oncogene c-Fos; proto-oncogene protein c-fos; Transcription factor AP-1 subunit c-Fos; unnamed protein product
Gene Aliases: AP-1; C-FOS; cFos; D12Rfj1; FOS; G0S7; p55
UniProt ID: (Human) P01100, (Mouse) P01101
Entrez Gene ID: (Human) 2353, (Mouse) 14281
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription cofactor binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
identical protein binding
sequence-specific DNA binding
macromolecular complex binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
R-SMAD binding
sequence-specific double-stranded DNA binding
promoter-specific chromatin binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor binding
transcription regulatory region DNA binding
protein heterodimerization activity
basic leucine zipper transcription factor
conditioned taste aversion
neural retina development
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
inflammatory response
transforming growth factor beta receptor signaling pathway
nervous system development
female pregnancy
response to xenobiotic stimulus
response to light stimulus
response to mechanical stimulus
response to gravity
response to toxic substance
regulation of gene expression
response to activity
skeletal muscle cell proliferation
cerebral cortex development
neuron differentiation
osteoclast differentiation
response to lipopolysaccharide
response to progesterone
response to insulin
cellular response to hormone stimulus
response to cytokine
cellular response to zinc ion starvation
cellular response to reactive oxygen species
response to immobilization stress
skeletal muscle cell differentiation
response to muscle stretch
response to ethanol
positive regulation of osteoclast differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
response to steroid hormone
response to corticosterone
myoblast proliferation
response to cAMP
SMAD protein signal transduction
cellular response to calcium ion
cellular response to tumor necrosis factor
cellular response to epidermal growth factor stimulus
cellular response to parathyroid hormone stimulus
cellular response to hypoxia
cellular response to transforming growth factor beta stimulus
medium-term memory
integrated stress response signaling
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
mononuclear cell differentiation
response to forskolin
cellular response to phorbol 13-acetate 12-myristate
cellular response to prolactin
cellular response to extracellular stimulus
response to drug
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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