PA1-833 detects cJun from mouse cells.
PA1-833 has been successfully used in Western blot and ChIP procedures. By Western blot, this antibody detects an ~40 kDa protein representing cJun in NIH 3T3 serum stimulated cells. A smaller band is also detected at ~26 kDa possibly representing a proteolytic fragment of cJun.
The PA1-833 immunogen is a synthetic peptide corresponding to residues T(91) P T P T Q F L C P K N V T D(105) of mouse cJun.
Reconstitute with PBS.
Fos and Jun dimerize to form Activator Protein-1 (AP-1), a transcriptional factor that binds to the 12-O-tetradecanoylphorbol 13-acetate (TPA) response element (TRE) of several cellular and viral genes including human collagenase, metallothionein IIa, stromelysin, interleukin 2, SV40 and polyoma. Fos and Jun contain the and quote;leucine-zipper' motif that allows for dimerization and an adjacent basic domain required for biological activity. The functionally active form of Fos is in a heterodimer with a member of the Jun family. While Jun family members can form functional homodimers, studies indicate that Fos family members do not self-associate and therefore do not bind DNA on their own. The various dimers differ in their ability to transactivate AP-1 dependent genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Activator protein 1; AH119; AP1; immediate early; Jun; Jun A; Jun Oncogene; Proto-oncogene c-Jun; Transcription factor AP-1; V-jun avian sarcoma virus 17 oncogene homolog
Gene Aliases: AP-1; c-jun; Jun; Junc
UniProt ID: (Mouse) P05627
Entrez Gene ID: (Mouse) 16476