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Invitrogen
c-Jun Polyclonal Antibody
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FIGURE: 1 / 3
c-Jun Antibody (PA5-144596) in ICC/IF
Immunocytochemistry analysis of c-Jun in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Samples were incubated with c-Jun polyclonal antibody (Product # PA5-144596) using a dilution of 1:500 (green). Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 μm.
Product Details
PA5-144596
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human c-Jun. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.43 mg/mL
Amount
43 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activator protein 1; AP1; cjun; enhancer-binding protein AP1; JUN; Jun activation domain binding protein; jun oncogene; JUND; p39; Proto-oncogene c-Jun; proto-oncogene cJun; transcription factor AP-1; Transcription factor AP-1 subunit Jun; Transcription factor Jun; V-jun avian sarcoma virus 17 oncogene homolog; v-jun sarcoma virus 17 oncogene homolog
Gene Aliases: AP-1; AP1; c-Jun; cJUN; JUN; p39
UniProt ID: (Human) P05412
Entrez Gene ID: (Human) 3725
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
RNA binding
GTPase activator activity
protein binding
enzyme binding
ubiquitin protein ligase binding
cAMP response element binding
identical protein binding
ubiquitin-like protein ligase binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
R-SMAD binding
general transcription initiation factor binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
angiogenesis
microglial cell activation
liver development
positive regulation of endothelial cell proliferation
outflow tract morphogenesis
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
apoptotic process
transforming growth factor beta receptor signaling pathway
JNK cascade
cell proliferation
negative regulation of cell proliferation
response to xenobiotic stimulus
positive regulation of epithelial cell migration
release from viral latency
monocyte differentiation
axon regeneration
response to endoplasmic reticulum stress
leading edge cell differentiation
response to muscle stretch
regulation of cell proliferation
positive regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of DNA binding
negative regulation of neuron apoptotic process
negative regulation by host of viral transcription
positive regulation by host of viral transcription
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of fibroblast proliferation
response to steroid hormone
regulation of cell cycle
SMAD protein signal transduction
eyelid development in camera-type eye
positive regulation of ERK1 and ERK2 cascade
cellular response to calcium ion
cellular response to anisomycin
integrated stress response signaling
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
positive regulation of vascular smooth muscle cell proliferation
positive regulation of DNA-templated transcription, initiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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