Immunohistochemistry analysis of c-Met showing staining in the cytoplasm and membrane of paraffin-embedded human lung adenocarcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a c-Met Rabbit Polyclonal Antibody (718000) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the C-terminal or the human c-Met protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||1:100-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The c-Met oncogene was originally isolated from a chemical carcinogen-treated human osteogenic sarcoma cell line by transfection analysis in NIH/3T3 cells. The Met proto-oncogene product was identified as a trans-membrane receptor-like protein with tyrosine kinase activity that is expressed in many tissues. The c-Met gene product has been identified as the cell surface receptor for hepatocyte growth factor, a plasminogen-like protein thought to be a humoral mediator of liver regeneration.
IP-MS enrichment of MET (LFQ intensity): MET was enriched 185-fold from BT549 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and c-MET antibody (Part No. 71-8000). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Absent and abundant MET immunoreactivity is associated with poor prognosis of patients with oral and oropharyngeal squamous cell carcinoma.
71-8000 was used in immunocytochemistry, immunohistochemistry - paraffin section, and western blot to learn the poor prognosis marker of patients with oral and oropharyngeal squamous cell carcinoma due to the absence or abundance of MET immunoreactivity
|De Herdt MJ,Willems SM,van der Steen B,Noorlag R,Verhoef EI,van Leenders GJ,van Es RJ,KoljenoviÄ S,Baatenburg de Jong RJ,Looijenga LH||Oncotarget (7:13167)||2016|
Ovarian cancer ascites enhance the migration of patient-derived peritoneal mesothelial cells via cMet pathway through HGF-dependent and -independent mechanisms.
71-8000 was used in western blot to suggest that HGF and EGFR signaling mediate ovarian cancer ascites-mediated migration of human peritoneal mesothelial cells
|Matte I,Lane D,Laplante C,Garde-Granger P,Rancourt C,Piché A||International journal of cancer (137:289)||2015|
p38 MAPK signaling underlies a cell-autonomous loss of stem cell self-renewal in skeletal muscle of aged mice.
71-8000 was used in immunocytochemistry to study the age-associated deregulation of the satellite cell homeostatic network to identify therapeutic targets.
|Bernet JD,Doles JD,Hall JK,Kelly Tanaka K,Carter TA,Olwin BB||Nature medicine (20:265)||2014|
Satellite cell number and cell cycle kinetics in response to acute myotrauma in humans: immunohistochemistry versus flow cytometry.
71-8000 was used in immunohistochemistry to develop a flow cytometric assay to count muscle satellite cell
|McKay BR,Toth KG,Tarnopolsky MA,Parise G||The Journal of physiology (588:3307)||2010|
|Not Applicable||Not Cited||
Acquisition of biologically relevant gene expression data by Affymetrix microarray analysis of archival formalin-fixed paraffin-embedded tumours.
71-8000 was used in immunohistochemistry - paraffin section to examine archival formalin-fixed paraffin-embedded tissue samples from primary extremity soft tissue sarcomas
|Linton KM,Hey Y,Saunders E,Jeziorska M,Denton J,Wilson CL,Swindell R,Dibben S,Miller CJ,Pepper SD,Radford JA,Freemont AJ||British journal of cancer (98:1403)||2008|
|Human||1:200||c-MET expression level in primary colon cancer: a predictor of tumor invasion and lymph node metastases.||Takeuchi H,Bilchik A,Saha S,Turner R,Wiese D,Tanaka M,Kuo C,Wang HJ,Hoon DS||Clinical cancer research : an official journal of the American Association for Cancer Research (9:1480)||2003|