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Proteintech
c-Met (Cytoplasmic) Polyclonal Antibody
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FIGURE: 1 / 12
c-Met (Cytoplasmic) Antibody (25869-1-AP) in IHC (P)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using (Product # 25869-1-AP) (c-Met (Cytoplasmic) antibody) at dilution of 1:1,000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Product Details
25869-1-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat
Published species
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
c-Met (Cytoplasmic) Fusion Protein Ag23140 (965-1085 aa encoded by BC130420)
Conjugate
Form
Liquid
Concentration
0.29 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
In Western blot, this antibody produces bands of unknown identity at 55 and 100 kDa.
Immunogen sequence: GSELVRYDA RVHTPHLDRL VSARSVSPTT EMVSNESVDY RATFPEDQFP NSSQNGSCRQ VQYPLTDMSP ILTSGDSDIS SPLLQNTVHI DLSALNPELV QAVQHVVIGP SSLIVHFNEV IG (965-1085 aa encoded by BC130420)
Target Information
MET (cMET) is a receptor-like tyrosine kinase whose dysregulation has been linked to many types of human malignancies. After activation of the ligand, MET interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3. There interactions lead to the activation of signaling cascades including RAS-ERK, PI3, kinase-AKT, and PLCgamma-PKC. MET plays a role in embryonic development including gastrulation, development of muscles and neurons, angiogenesis, and kidney formation. It also plays a role in adults including wound healing, organ regeneration, and tissue remodeling. MET has been linked to cancers including gastric, renal, and breast; therefore, making it a target for cancer therapeutics and diagnostic testing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: c-Met; cMet; EC 2.7.10.1; Hepatocyte growth factor receptor; HGF receptor; HGF-SF receptor; HGF/SF receptor; met proto-oncogene (hepatocyte growth factor receptor); met proto-oncogene tyrosine kinase; Met; protein tyrosin kinase; oncogene MET; Proto-oncogene c-Met; sc MET; Scatter factor receptor; SF receptor; soluble c met; Tyrosine-protein kinase Met
Gene Aliases: AI838057; AUTS9; c-Met; DFNB97; HGF; HGFR; MET; Par4; RCCP2
UniProt ID: (Dog) Q75ZY9, (Human) P08581, (Rat) P97523
Entrez Gene ID: (Dog) 403438, (Human) 4233, (Rat) 24553, (Mouse) 17295
ATP binding
semaphorin receptor activity
protein tyrosine kinase activity
hepatocyte growth factor-activated receptor activity
protein binding
protein phosphatase binding
protein kinase activity
beta-catenin binding
protein complex binding
phospholipase binding
phosphatidylinositol 3-kinase binding
protein heterodimerization activity
nucleotide binding
transmembrane receptor protein tyrosine kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
transmembrane signal receptor
peptidyl-tyrosine phosphorylation
hepatocyte growth factor receptor signaling pathway
positive chemotaxis
semaphorin-plexin signaling pathway
positive regulation of endothelial cell chemotaxis
endothelial cell morphogenesis
signal transduction
cell surface receptor signaling pathway
cell proliferation
negative regulation of autophagy
positive regulation of transcription from RNA polymerase II promoter
branching morphogenesis of an epithelial tube
negative regulation of hydrogen peroxide-mediated programmed cell death
negative regulation of transcription from RNA polymerase II promoter
activation of MAPK activity
neuron migration
liver development
placenta development
brain development
muscle organ development
skeletal muscle tissue development
cell aging
lactation
male gonad development
response to wounding
response to acidic pH
positive regulation of gene expression
positive regulation of peptidyl-threonine phosphorylation
negative regulation of peptidyl-threonine phosphorylation
positive regulation of glucose transport
positive regulation of neuron projection development
oligodendrocyte development
response to organic cyclic compound
muscle cell migration
myotube differentiation
central nervous system neuron differentiation
sperm motility
adult behavior
pancreas development
positive regulation of peptidyl-serine phosphorylation
response to drug
glucose homeostasis
response to peptide hormone
positive regulation of DNA replication
positive regulation of mitotic nuclear division
protein autophosphorylation
positive regulation of dendrite morphogenesis
modulation of synaptic transmission
myoblast proliferation
excitatory postsynaptic potential
regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling
cellular response to inorganic substance
cellular response to arsenic-containing substance
cellular response to glucose stimulus
cellular response to interleukin-6
cellular response to growth factor stimulus
cellular response to peptide hormone stimulus
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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