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FIGURE: 1 / 4
c-Raf Antibody (711198) in ICC/IF
For immunofluorescence analysis, HeLa cells were fixed and permeabilized for detection of endogenous RAF1 using Anti- RAF1 Recombinant Rabbit Superclonal™ Antibody (Product # 711198, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of RAF1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S3693... View More
Product Details
711198
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
8HCLC
Immunogen
Peptide corresponding to human c-Raf [aa334-aa343]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Dog.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-Raf proto-oncogene, serine/threonine kinase; cRaf; Craf1 transforming; EC 2.7.11.1; kinase Raf1; murine sarcoma 3611 oncogene 1; Oncogene RAF1; OTTHUMP00000207813; OTTHUMP00000209389; Proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; RAF proto-oncogene serine/threonine-protein kinase; Raf-1; sarcoma 3611 oncogene; unnamed protein product; v-raf-1 leukemia viral oncogene 1; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1; vraf1 murine le
Gene Aliases: 6430402F14Rik; AA990557; BB129353; c-Raf; CMD1NN; CRAF; Craf1; D830050J10Rik; NS5; RAF; Raf-1; RAF1; v-Raf
UniProt ID: (Human) P04049, (Mouse) Q99N57
Entrez Gene ID: (Human) 5894, (Mouse) 110157
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
MAP kinase kinase kinase activity
protein binding
ATP binding
zinc ion binding
adenylate cyclase activator activity
kinase activity
transferase activity
enzyme binding
small GTPase binding
identical protein binding
metal ion binding
protein serine kinase activity
receptor signaling protein activity
transferase activity, transferring phosphorus-containing groups
Ras GTPase binding
mitogen-activated protein kinase kinase binding
protein heterodimerization activity
non-receptor serine/threonine protein kinase
MAPK cascade
cellular glucose homeostasis
protein phosphorylation
apoptotic process
signal transduction
negative regulation of cell proliferation
insulin receptor signaling pathway
extrinsic apoptotic signaling pathway via death domain receptors
Schwann cell development
cell differentiation
thyroid gland development
negative regulation of protein complex assembly
positive regulation of peptidyl-serine phosphorylation
somatic stem cell population maintenance
regulation of Rho protein signal transduction
insulin secretion involved in cellular response to glucose stimulus
response to muscle stretch
ERBB2-ERBB3 signaling pathway
wound healing
myelination
regulation of apoptotic process
negative regulation of apoptotic process
positive regulation of MAPK cascade
type B pancreatic cell proliferation
intermediate filament cytoskeleton organization
regulation of cell differentiation
positive regulation of transcription from RNA polymerase II promoter
insulin-like growth factor receptor signaling pathway
neurotrophin TRK receptor signaling pathway
thymus development
face development
interferon-gamma-mediated signaling pathway
death-inducing signaling complex assembly
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
activation of MAPKK activity
response to hypoxia
positive regulation of protein phosphorylation
phosphorylation
intracellular signal transduction
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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