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Agrisera
eEF1B-alpha2/elongation factor 1B-alpha 2 Polyclonal Antibody
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FIGURE: 1 / 1
eEF1B-alpha2/elongation factor 1B-alpha 2 Antibody (AS10-679) in WB
10 µg total protein extracted from 15 day old Arabidopsis thaliana seedlings that were either kept at 22ºC (1) or heat treated at 38ºC for 2 hours prior to protein extraction (2). As positive control 10 ng of recombinant elongation factor proteins alpha 1 (3) and alpha 2 (4) were separated side by side with the plant samples on 11% SDS-PAGE and blotted to nitrocellulose (Bio-Rad). Blots were blocked followi... View More
Product Details
AS10-679
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant eEF1B-alpha2 protein from Arabidopsis thaliana with no affinity tag.
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 200 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
EF1B2, also called elongation factor 1 beta 2, is a cytosolic subunit of the EF1B complex that serves as a guanine nucleotide exchange factor for EF1A, reloading EF1A with GTP to sustain delivery of aminoacyl tRNAs to ribosomes during translation elongation in plants. EF1B2 partners with EF1B gamma and EF1B delta to form a stable exchange complex, and its activity influences translational throughput, growth, and stress adaptation. Expression of EF1B2 is generally strong in actively dividing and expanding tissues and can shift with environmental stresses, hormone signals, and developmental transitions. Beyond translation, EF1B2 and its complex contribute to cytoskeletal dynamics and vesicle trafficking via EF1A interactions, linking protein synthesis capacity to broader cellular organization. When used as a reference gene, EF1B2 requires validation under specific experimental conditions because its expression can vary with treatments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2810017J07Rik; Eef1b; EF-1-beta
Disclaimer
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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