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          • Primary Antibodies ›
          • eGFP Antibodies

          Invitrogen

          eGFP Polyclonal Antibody

          Advanced Verification
          11 Published Figures
          29 References
          View all (10) eGFP antibodies

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          Cite eGFP Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (4)
          • Published Figures (11)
          • Advanced Verification (1)
          eGFP Antibody in Immunocytochemistry (ICC/IF)
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          eGFP Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 16

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          eGFP Antibody (CAB4211) in ICC/IF

          Immunofluorescent analysis of GFP Tag was performed using H3-GFP construct transfected in HEK-293E cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with eGFP Polyclonal Antibody (Product # CAB4211) at 1:100 dilution and Histone H3 Monoclonal Antibody (865R2) Product # AHO1432) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          eGFP Antibody in Immunocytochemistry (ICC/IF)
          eGFP Antibody in Immunocytochemistry (ICC/IF)
          eGFP Antibody in Western Blot (WB)
          eGFP Antibody in Western Blot (WB)
          eGFP Antibody in Immunohistochemistry (IHC)
          eGFP Antibody in Immunohistochemistry (IHC)
          eGFP Antibody in Immunohistochemistry (IHC)
          eGFP Antibody in Immunohistochemistry (IHC)
          eGFP Antibody in Immunohistochemistry (IHC)
          eGFP Antibody in Immunohistochemistry (IHC)
          eGFP Antibody in Western Blot (WB)
          eGFP Antibody in Western Blot (WB)
          eGFP Antibody in Western Blot (WB)
          eGFP Antibody in Western Blot (WB)
          eGFP Antibody in Western Blot (WB)
          eGFP Antibody

          Product Details

          CAB4211

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1 µg/mL
          View 12 publications 12 publications

          Immunohistochemistry (IHC)

          -
          View 10 publications 10 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 1 publication 1 publication

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 2 publications 2 publications

          Immunocytochemistry (ICC/IF)

          1:20-1:200
          View 2 publications 2 publications

          Flow Cytometry (Flow)

          -
          View 1 publication 1 publication

          ChIP assay (ChIP)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Tag

          Published species

          Tag

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Purified recombinant eGFP

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          BBS, pH 8.4

          Contains

          no preservative

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10709851

          Product Specific Information

          CAB4211 detects eGFP in samples.

          CAB4211 has been successfully used in Western Blot and ICC/IF applications.

          The CAB4211 immunogen is purified recombinant eGFP.

          Target Information

          eGFP (green fluorescent protein) fluorescent protein.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: eGFP; egfp tag; GFP; GFP tag; GFP2; green fluorescence; green fluorescent; Green Fluoresecent Protein; Turbo eGFP

          View more View less

          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
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          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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