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Invitrogen
eIF3e Polyclonal Antibody
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FIGURE: 1 / 3
eIF3e Antibody (PA5-112192) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of eIF3e in U2OS cells. Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with eIF3e Polyclonal Antibody (Product # PA5-112192) (1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
Product Details
PA5-112192
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived Human EIF3E fragment
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS, pH 7
Contains
0.03% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: eIF-3 p48; eIF3e; EIF3S6; Eukaryotic translation initiation factor 3 subunit 6; Eukaryotic translation initiation factor 3 subunit E; eukaryotic translation initiation factor 3, subunit 6 (48kD); eukaryotic translation initiation factor 3, subunit 6 48kDa; mammary tumor-associated protein INT6; murine mammary tumor integration site 6 (oncogene homolog); similar to mouse Int-6; unnamed protein product; Viral integration site protein INT-6 homolog
Gene Aliases: eIF3-p46; EIF3-P48; EIF3E; EIF3S6; INT6
UniProt ID: (Human) P60228
Entrez Gene ID: (Human) 3646
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
formation of cytoplasmic translation initiation complex
cytoplasmic translational initiation
translation
translational initiation
regulation of translational initiation
positive regulation of translation
negative regulation of translational initiation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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