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Invitrogen
eIF3h Polyclonal Antibody
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FIGURE: 1 / 2
eIF3h Antibody (PA5-89202) in WB
Western blot analysis of eIF3h in extracts of various cell lines. Samples were incubated with eIF3h Polyclonal antibody (Product # PA5-89202) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-89202
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human EIF3H (NP_0037471)
Conjugate
Form
Liquid
Concentration
1.02 mg/mL
Amount
102 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: NCFPFPQHTE DDADFDEVQY QMEMMRSLRH VNIDHLHVGW YQSTYYGSFV TRALLDSQFS YQHAIEESVV LIYDPIKTAQ GSLSLKAYRL TPKLMEVCKE KDFSPEALKK ANITFEYMFE EVPIVIKNSH LINVLMWELE KKSAVADKHE LLSLASSNHL GKNLQLLMDR VDEMSQDIVK YNTYMRNTSK QQQQKHQYQQ RRQQENMQRQ SRGEPPLPEE DLSKLFKPPQ PPARMDSLLI AGQINTYCQN IKEFTAQNLG K; Positive Samples: HL-60, HT-29, NIH/3T3, HeLa, Jurkat, Mouse spleen, Mouse testis, Mouse heart, Rat spleen; Cellular Location: Cytoplasm
Target Information
eIF3H is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: eIF-3 gamma; eIF-3-gamma; eIF3 p40 subunit; eIF3 p40 subunit {ECO:0000255|HAMAP-Rule:MF_03007}; eIF3-p40; eIF3h; eIF3h {ECO:0000255|HAMAP-Rule:MF_03007}; eIF3p40; EIF3S3; Eukaryotic translation initiation factor 3 subunit 3; eukaryotic translation initiation factor 3 subunit 3 {ECO:0000255|HAMAP-Rule:MF_03007}; Eukaryotic translation initiation factor 3 subunit H; eukaryotic translation initiation factor 3 subunit H {ECO:0000255|HAMAP-Rule:MF_03007}; eukaryotic translation initiation factor 3, subunit 2 (beta, 36kD); eukaryotic translation initiation factor 3, subunit 3 (gamma); eukaryotic translation initiation factor 3, subunit 3 (gamma, 40kD); eukaryotic translation initiation factor 3, subunit 3 gamma, 40kDa; MGC102958; unnamed protein product
Gene Aliases: 1110008A16Rik; 40kD; 9430017H16Rik; EIF3-gamma; EIF3-P40; EIF3H; EIF3S3
UniProt ID: (Human) O15372, (Rat) Q6P9U8, (Mouse) Q91WK2
Entrez Gene ID: (Human) 8667, (Rat) 299899, (Mouse) 68135
formation of cytoplasmic translation initiation complex
cytoplasmic translational initiation
translation
translational initiation
regulation of translational initiation
negative regulation of proteasomal ubiquitin-dependent protein catabolic process
formation of translation preinitiation complex
biological_process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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