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Invitrogen
eIF4A2 Polyclonal Antibody
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FIGURE: 1 / 4
eIF4A2 Antibody (PA5-112372) in ICC/IF
Immunocytochemichal analysis of eIF4A2 in A549 cells using a Polyclonal antibody (Product # PA5-112372) at a dilution of 1:100. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were also counter-stained with DAPI.
Product Details
PA5-112372
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human Eukaryotic initiation factor 4A-II protein (1-407AA)
Conjugate
Form
Liquid
Concentration
0.6 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
eIF4A2 is a 407 amino acid ATP-dependent RNA helicase belonging to the DEAD box helicase family. It contains one helicase ATP-binding domain and a helicase C-terminal domain. eIF4A2, a subunit of the eIF4F complex, acts as a protein-synthesis initiation factor and thus plays an important role in the binding of mRNA to the 43S pre-initiation complex of ribosomes. Human eIF4A2 interacts with the NS5B protein of Hepatitis C virus (HCV) and facilitates the genomic RNA synthesis of NS5B protein by unwinding the secondary structure of the HCV genome. It is ubiquitously expressed in most of normal tissues, but in variable amounts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP dependent RNA helicase eIF4A 2; ATP-dependent RNA helicase eIF4A-2; eIF-4A-II; Eukaryotic initiation factor 4A-II; eukaryotic translation initiation factor 4A isoform 2; N-terminally processed; unnamed protein product
Gene Aliases: BM-010; DDX2B; eIF-4A-II; EIF4A; eIF4A-II; EIF4A2; EIF4F; NEDHSS
UniProt ID: (Human) Q14240
Entrez Gene ID: (Human) 1974
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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