Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human small intestine stained using a eIF4A2 polyclonal antibody (Product # PA5-23264) at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
|Tested species reactivity||Dog, Chicken, Human, Mouse, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (69 KGYDVIAQAQSGTGKTAT 86) of eIF4A2 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Western Blot (WB)||0.025-0.05 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
eIF4A2 is a 407 amino acid ATP-dependent RNA helicase, belonging to the DEAD box helicase family. It contains one helicase ATP-binding domain and a helicase C-terminal domain. eIF4A2, a subunit of the eIF4F complex, acts as a protein-synthesis initiation factor and thus plays an important role in the binding of mRNA to the 43S pre-initiation complex of ribosomes. Research is carried out to elucidate the potential role of eIF4A2 in glucose homeostasis and also in type 2 diabetes in the presence of metabolic stress where eIF4A2 controls specific mRNA translation and protein synthesis rate in pancreatic beta-cells. Human eIF4A2 interacts with the NS5B protein of Hepatitis C virus (HCV) and facilitates the genomic RNA synthesis of NS5B protein by unwinding the secondary structure of the HCV genome. It is ubiquitously expressed in most of normal tissues, but in variable amount.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.