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Invitrogen
eIF4E Polyclonal Antibody
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FIGURE: 1 / 4
eIF4E Antibody (PA5-77960) in ICC/IF
eIF4E Polyclonal Antibody detects eIF4E protein at cytoplasm by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: eIF4E protein stained by eIF4E Polyclonal Antibody (Product # PA5-77960) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-77960
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human eIF4E. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.52 mg/mL
Amount
52 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: 293T, 293T cytoplasm fraction extract, mouse colon
Predicted Reactivity: Rat (99%), Xenopus laevis (91%), Rabbit (99%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (99%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
eIF4E is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A, that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: eIF-4E; eIF-4F 25 kDa subunit; Eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E-like 1; MGC111573; mRNA cap-binding protein; OTTHUMP00000219703; OTTHUMP00000219704; OTTHUMP00000219705; unnamed protein product
Gene Aliases: AUTS19; CBP; EG668879; eIF-4E; EIF4E; Eif4e-ps; EIF4E1; EIF4EL1; EIF4F; If4e
UniProt ID: (Human) P06730, (Mouse) P63073
Entrez Gene ID: (Human) 1977, (Mouse) 13684
RNA cap binding
RNA 7-methylguanosine cap binding
RNA binding
translation initiation factor activity
protein binding
enzyme binding
eukaryotic initiation factor 4G binding
mRNA cap binding
DNA-binding transcription factor binding
poly(A) RNA binding
translation factor
translation initiation factor
translational protein
G1/S transition of mitotic cell cycle
behavioral fear response
mRNA export from nucleus
translation
translational initiation
regulation of translation
negative regulation of autophagy
negative regulation of translation
stem cell population maintenance
neuron differentiation
negative regulation of neuron differentiation
positive regulation of mitotic cell cycle
mRNA transport
nuclear export
cellular response to dexamethasone stimulus
regulation of translation at postsynapse, modulating synaptic transmission
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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