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Invitrogen
eIF4G3 Polyclonal Antibody
FIGURE: 1 / 13
eIF4G3 Antibody (PA5-31101) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of eIF4G3 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: eIF4G3 Polyclonal Antibody (Product # PA5-31101) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-31101
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein fragment corresponding to a region within amino acids 1 and 203 of Human EIF4G3
Conjugate
Form
Liquid
Concentration
0.26 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2548575
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, mouse testis.
Predicted reactivity: Human (99%), Mouse (96%), Rat (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene is thought to be part of the eIF4F protein complex, which is involved in mRNA cap recognition and transport of mRNAs to the ribosome. Interestingly, a microRNA (miR-520c-3p) has been found that negatively regulates synthesis of the encoded protein, and this leads to a global decrease in protein translation and cell proliferation. Therefore, this protein is a key component of the anti-tumor activity of miR-520c-3p.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: eIF-4-gamma 3; eIF-4-gamma II; eIF-4G 3; eIF4G 3; eIF4GII; Eukaryotic translation initiation factor 4 gamma 3; RP11-190H11.1
Gene Aliases: 1500002J22Rik; 4833436O05; 4930523M17Rik; eIF-4G 3; eIF4G 3; EIF4G3; eIF4GII; G1-419-52; repro8
UniProt ID: (Human) O43432, (Mouse) Q80XI3
Entrez Gene ID: (Human) 8672, (Mouse) 230861, (Rat) 298573
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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