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Invitrogen
eIF6 Polyclonal Antibody
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FIGURE: 1 / 9
eIF6 Antibody (PA5-37371) in ICC/IF
Immunofluorescence analysis of eIF6 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with eIF6 Rabbit Polyclonal Antibody(Product # PA5-37371) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034... View More
Product Details
PA5-37371
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant full length human eIF6
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody detects endogenous protein at a molecular weight of 27 kDa.
Purity is >95% by SDS-PAGE.
Target Information
Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple transcript variants encoding two different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B(2)GCN homolog; B4 integrin interactor; CAB; eIF-6; eukaryotic translation initiation factor 3A; Eukaryotic translation initiation factor 6; ITGB4BP; p27 beta-4 integrin-binding protein; p27(BBP); ribosome antiassociation factor; eIF6; translation initiation factor 6; unnamed protein product
Gene Aliases: b(2)gcn; CAB; eIF-6; EIF3A; EIF6; ITGB4BP; OK/SW-cl.27; p27(BBP); p27BBP
UniProt ID: (Human) P56537
Entrez Gene ID: (Human) 3692
ribosomal subunit export from nucleus
maturation of 5.8S rRNA
maturation of LSU-rRNA
regulation of glycolytic process
translation
translational initiation
response to insulin
gene silencing by miRNA
miRNA mediated inhibition of translation
ribosome biogenesis
mature ribosome assembly
ribosomal large subunit biogenesis
regulation of fatty acid biosynthetic process
regulation of megakaryocyte differentiation
positive regulation of translation
assembly of large subunit precursor of preribosome
regulation of reactive oxygen species metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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