|Tested species reactivity||Bovine, Dog, Human, Mouse, Pig, Rat|
|Published species reactivity||Dog, Rabbit, Rat, Fruit fly, Amphibian, Bovine, Cat, Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues S(1179) L Q E R Q L R G A V P W A F D(1194) of human eNOS.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-037 detects endothelial nitric oxide synthase (eNOS) from bovine, canine, human, mouse, porcine and rat tissues as well as recombinant protein. This antibody does not detect human iNOS or rat nNOS.
PA1-037 has been successfully used in Western blot and immunohistochemistry (paraffin and frozen) procedures. By Western blot, this antibody detects an ~140 kDa protein representing eNOS from human endothelial cells. By Western blot PA1-037 also detects eNOS in rat lung, brain, and canine cardiac microsomes. Immunohistochemical staining of eNOS in human vascular endothelial cells with PA1-037 results in prominent staining of the epithelial cells.
The PA1-037 immunogen is a synthetic peptide corresponding to residues S(1179) L Q E R Q L R G A V P W A F D(1194) of human eNOS.
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mitochondria DNA mutations cause sex-dependent development of hypertension and alterations in cardiovascular function.
PA1-037 was used in immunohistochemistry - paraffin section to study the effects of mtDNA mutations on cardiovascular function using mice with a mutation in the mtDNA polymerase-gamma
|Golob MJ,Tian L,Wang Z,Zimmerman TA,Caneba CA,Hacker TA,Song G,Chesler NC||Journal of biomechanics (48:405)||2015|
The impact of vessel clamps on endothelial integrity and function of saphenous vein grafts.
PA1-037 was used in immunohistochemistry - paraffin section to investigate the effect of plastic and metal clamps on the endothelial integrity and function of saphenous vein graft
|Onan B,Erkanli K,Onan IS,Ersoy B,Canillioglu YE,Senturk GE,Hurdag C,Yeniterzi M||Annals of vascular surgery (28:1113)||2014|
Pressure stress reduces inducible NO synthase expression by interleukin-1ß stimulation in cultured rat vascular smooth muscle cells.
PA1-037 was used in western blot to study the mechanisms by which pulsate pressure stress impaires IL-1beta-induced iNOS expression and NOS production in cultured rat vascular smooth muscle cells
|Machida T,Iizuka K,Shinohara K,Hatakeyama N,Nakano K,Kubo Y,Hirafuji M||European journal of pharmacology (731:44)||2014|
Xanthine oxidase inhibition prevents atrial fibrillation in a canine model of atrial pacing-induced left ventricular dysfunction.
PA1-037 was used in western blot to study the effect of the xanthine oxidase inhibitor allopurine on atrial fibrillation in a canine model of tachycardia-induced cardiomyopathy
|Sakabe M,Fujiki A,Sakamoto T,Nakatani Y,Mizumaki K,Inoue H||Journal of cardiovascular electrophysiology (23:1130)||2012|
The effect of cadmium toxicity on renal nitric oxide synthase isoenzymes.
PA1-037 was used in western blot to study the effect of cadmium of the expression of NOS isozymes in the rat kidney
|Soyupek S,Oksay T,Sütçü R,Armagan A,Gökalp O,Perk H,Delibas N||Toxicology and industrial health (28:624)||2012|
Human primary lung endothelial cells in culture.
PA1-037 was used in immunohistochemistry and western blot to develop methods for the isolation and culture of human primary pulmonary endothelial cells
|Comhair SA,Xu W,Mavrakis L,Aldred MA,Asosingh K,Erzurum SC||American journal of respiratory cell and molecular biology (46:723)||2012|
Time-dependent alterations of VEGF and its signaling molecules in acute lung injury in a rat model of sepsis.
PA1-037 was used in western blot to investigate the alterations of vascular endothelial growth factor signaling pathway in acute lung injury in a rat sepsis model
|Jesmin S,Zaedi S,Islam AM,Sultana SN,Iwashima Y,Wada T,Yamaguchi N,Hiroe M,Gando S||Inflammation (35:484)||2012|
Impairment of cardiac insulin signaling in fructose-fed ovariectomized female Wistar rats.
PA1-037 was used in western blot to investigate effect of fructose diet on cardiac insulin signaling
|Zakula Z,Koricanac G,Tepavcevic S,Stojiljkovic M,Milosavljevic T,Isenovic ER||European journal of nutrition (50:543)||2011|
Celecoxib but not the combination of celecoxib+atorvastatin prevents the development of monocrotaline-induced pulmonary hypertension in the rat.
PA1-037 was used in western blot to study the effect of celecoxib and atorvastatin on monocrotaline-induced pulmonary hypertension in the rodents
|Rakotoniaina Z,Guerard P,Lirussi F,Rochette L,Dumas M,Goirand F,Bardou M||Naunyn-Schmiedeberg's archives of pharmacology (378:241)||2008|
Cardioprotection mediated by rosiglitazone, a peroxisome proliferator-activated receptor gamma ligand, in relation to nitric oxide.
PA1-037 was used in western blot to investigate the role of nitric oxide on cardioprotective effect by rosiglitazone
|Gonon AT,Bulhak A,Labruto F,Sjöquist PO,Pernow J||Basic research in cardiology (102:80)||2007|
Endocardial endothelium in the avascular frog heart: role for diffusion of NO in control of cardiac O2 consumption.
PA1-037 was used in western blot to study the role of nitric oxide (NO) in the control of cardiac O2 consumption
|Adler A,Huang H,Wang Z,Conetta J,Levee E,Zhang X,Hintze TH||American journal of physiology. Heart and circulatory physiology (287:H14)||2004|
Excessive zinc intake elevates systemic blood pressure levels in normotensive rats--potential role of superoxide-induced oxidative stress.
PA1-037 was used in western blot to study the potential mechanism by which excessive zinc intake elevates systemic blood pressure levels in normotensive rats
|Yanagisawa H,Sato M,Nodera M,Wada O||Journal of hypertension (22:543)||2004|
Molecular mechanisms of increased nitric oxide (NO) in asthma: evidence for transcriptional and post-translational regulation of NO synthesis.
PA1-037 was used in western blot to investigate the mechanisms leading to increased NO synthesis in the human asthmatic airway.
|Guo FH,Comhair SA,Zheng S,Dweik RA,Eissa NT,Thomassen MJ,Calhoun W,Erzurum SC||Journal of immunology (Baltimore, Md. : 1950) (164:5970)||2000|
Interaction between caveolin-1 and the reductase domain of endothelial nitric-oxide synthase. Consequences for catalysis.
PA1-037 was used in western blot to study the role of the cav-1 scaffolding domain peptide during the nitric-oxide synthesis
|Ghosh S,Gachhui R,Crooks C,Wu C,Lisanti MP,Stuehr DJ||The Journal of biological chemistry (273:22267)||1998|
Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase.
PA1-037 was used in western blot to investigate the characteristics of nitric oxide synthase in vivo
|Bredt DS,Hwang PM,Glatt CE,Lowenstein C,Reed RR,Snyder SH||Nature (351:714)||1991|
Protective effect of curcumin on cyclosporin A-induced endothelial dysfunction, antioxidant capacity, and oxidative damage.
PA1-037 was used in immunohistochemistry to study the ability of curcumin to protect against aortic endothelial dysfunction induced by cyclosporin A in a rat model
|Sagiroglu T,Kanter M,Yagci MA,Sezer A,Erboga M||Toxicology and industrial health (30:316)||2014|
Endothelial and inducible nitric oxide synthase (NOS) immunoreactivity and NOS-associated NADPH-diaphorase histochemistry in the domestic cat (Felis catus) testis.
PA1-037 was used in immunohistochemistry to study the localization of cat testicular eNOS, iNOS and NOS-associated NADPH diaphorase
|Liman N,Alan E,Beyaz F,Gürbulak K||Theriogenology (80:1017)||2013|
Effects of chronic SDMA infusion on glomerular filtration rate, blood pressure, myocardial function and renal histology in C57BL6/J mice.
PA1-037 was used in immunohistochemistry to study kidney function, blood pressure and cardiac performance following chronic infusion of symmetrical dimethylarginine in a murine model
|Veldink H,Faulhaber-Walter R,Park JK,Martens-Lobenhoffer J,Bode-Böger S,Schuett H,Haghikia A,Hilfiker-Kleiner D,Kielstein JT||Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association (28:1434)||2013|
Sildenafil protects against nitric oxide deficiency-related nephrotoxicity in cyclosporine A treated rats.
PA1-037 was used in immunohistochemistry to study the role of the NO pathway in the mechanism by which sildenafil protects against cyclosprine A-induced nephrotoxicity in rats
|Abdel-latif RG,Morsy MA,El-Moselhy MA,Khalifa MA||European journal of pharmacology (705:126)||2013|
Effects of Crataegus microphylla on vascular dysfunction in streptozotocin-induced diabetic rats.
PA1-037 was used in immunohistochemistry to study the mechanisms underlying the beneficial vascular effects of Crataegus microphylla extracts in a rat model of diabetes
|Topal G,Koç E,Karaca C,Altu¿ T,Ergin B,Demirci C,Meliko¿lu G,Meriçli AH,Kucur M,Ozdemir O,Uyde¿ Do¿an BS||Phytotherapy research : PTR (27:330)||2013|
High-risk human papillomavirus-induced expression of endothelial and inducible nitric oxide synthase in human uterine cervix.
PA1-037 was used in immunohistochemistry to study the cervical expression of eNOS and iNOS in women with high-risk HPV infection
|Rahkola-Soisalo P,Savolainen-Peltonen H,Väisänen-Tommiska M,Butzow R,Ylikorkala O,Mikkola TS||Annals of medicine (45:79)||2013|
The effects of PDE5 inhibitory drugs on renal ischemia/reperfusion injury in rats.
PA1-037 was used in immunohistochemistry to study the effects of the PDE5 inhibitors Tadalfil and Sildenafil on rat renal ischemia/reperfusion injury
|Küçük A,Yucel M,Erkasap N,Tosun M,Koken T,Ozkurt M,Erkasap S||Molecular biology reports (39:9775)||2012|
Low tidal volume protects pulmonary vasomotor function from "second-hit" injury in acute lung injury rats.
PA1-037 was used in immunohistochemistry to test if low tidal volume ventilation protects pulmonary vascular function
|Pan C,Wang J,Liu W,Liu L,Jing L,Yang Y,Qiu H||Respiratory research (13:null)||2012|
Expression of NOS and VEGF in feline mammary tumours and their correlation with angiogenesis.
PA1-037 was used in immunohistochemistry to study the expression of eNOS, iNOS and VEGF and their correlation with angiogenesis in feline mammary tumors
|Islam MS,Matsumoto M,Hidaka R,Miyoshi N,Yasuda N||Veterinary journal (London, England : 1997) (192:338)||2012|
Immunohistochemical study of the angiogenetic network of VEGF, HIF1¿, VEGFR-2 and endothelial nitric oxide synthase (eNOS) in human breast cancer.
PA1-037 was used in immunohistochemistry to study the expression of VEGF, VEGFR-2, HIF1-alpha and eNOS in invasive ductal carcinoma
|Kafousi M,Vrekoussis T,Tsentelierou E,Pavlakis K,Navrozoglou I,Dousias V,Sanidas E,Tsiftsis D,Georgoulias V,Stathopoulos EN||Pathology oncology research : POR (18:33)||2012|
A modest decrease in endothelial NOS in mice comparable to that associated with human NOS3 variants exacerbates diabetic nephropathy.
PA1-037 was used in immunohistochemistry to study the effects of reduced eNOS on diabetic nephropathy in mice and the significance with respect to human eNOS polymorphisms
|Wang CH,Li F,Hiller S,Kim HS,Maeda N,Smithies O,Takahashi N||Proceedings of the National Academy of Sciences of the United States of America (108:2070)||2011|
Oxidative and nitrosative stress and progression of diabetic nephropathy in type 2 diabetes.
PA1-037 was used in immunohistochemistry to study the role of oxidative and nitrosative stress in the progression of diabetic nephropathy in type 2 diabetes
|Fujii H,Kono K,Nakai K,Goto S,Komaba H,Hamada Y,Shinohara M,Kitazawa R,Kitazawa S,Fukagawa M||American journal of nephrology (31:342)||2010|
Mononuclear phagocyte system depletion blocks interstitial tonicity-responsive enhancer binding protein/vascular endothelial growth factor C expression and induces salt-sensitive hypertension in rats.
PA1-037 was used in immunohistochemistry to investigate the importance of mononuclear phagocyte system cells in regulation of interstitial volume and blood pressure homeostasis
|Machnik A,Dahlmann A,Kopp C,Goss J,Wagner H,van Rooijen N,Eckardt KU,Müller DN,Park JK,Luft FC,Kerjaschki D,Titze J||Hypertension (Dallas, Tex. : 1979) (55:755)||2010|
Distribution of arteriolovenous vessels, capillaries and eNOS expression in the bovine corpus luteum during the estrous cycle: a possible implication of different sensitivity by luteal phase to PGF(2alpha) in the increase of luteal blood flow.
PA1-037 was used in immunohistochemistry to investigate the change of the expression pattern of luteal vasculatures and eNOS in the CL during bovine estrous cycle
|Shirasuna K,Asahi T,Sasaki M,Shimizu T,Miyamoto A||The Journal of reproduction and development (56:124)||2010|
Glucose degradation products result in cardiovascular toxicity in a rat model of renal failure.
PA1-037 was used in immunohistochemistry to study the cardiovascular toxicity of glucose degradation products in a rat model of renal failure
|Müller-Krebs S,Kihm LP,Zeier B,Gross ML,Wieslander A,Haug U,Zeier M,Schwenger V||Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis (30:35)||2010|
|Fruit fly||Not Cited||
The STAT pathway mediates late-phase immunity against Plasmodium in the mosquito Anopheles gambiae.
PA1-037 was used in immunohistochemistry to show the cellular localization of NOS in infected midguts of An. gambiae
|Gupta L,Molina-Cruz A,Kumar S,Rodrigues J,Dixit R,Zamora RE,Barillas-Mury C||Cell host and microbe (5:498)||2009|
Sarpogrelate hydrochloride reduced intimal hyperplasia in experimental rabbit vein graft.
PA1-037 was used in immunohistochemistry to study effect of the selective 5-HT(2A) receptor antagonist sarpogrelate hydrochloride on intimal hyperplasia in experimental rabbit vein graft
|Kodama A,Komori K,Hattori K,Yamanouchi D,Kajikuri J,Itoh T||Journal of vascular surgery (49:1272)||2009|
Prostaglandin F2alpha increases endothelial nitric oxide synthase in the periphery of the bovine corpus luteum: the possible regulation of blood flow at an early stage of luteolysis.
PA1-037 was used in immunohistochemistry to study how the increase in blood flow observed at periphery of bovine CL during the early stage of luteolysis is regulated by eNOS and NO
|Shirasuna K,Watanabe S,Asahi T,Wijayagunawardane MP,Sasahara K,Jiang C,Matsui M,Sasaki M,Shimizu T,Davis JS,Miyamoto A||Reproduction (Cambridge, England) (135:527)||2008|
Nitric oxide synthase and NMDA receptor expressions in cavernoma tissues with epileptogenesis.
PA1-037 was used in immunohistochemistry to study the expression of NOS and NMDA receptor in cavernoma tissues with epileptogenesis
|Kamida T,Takeda Y,Fujiki M,Abe T,Abe E,Kobayashi H||Acta neurologica Scandinavica (116:368)||2007|
Reduced photoreceptor damage after photodynamic therapy through blockade of nitric oxide synthase in a model of choroidal neovascularization.
PA1-037 was used in immunohistochemistry to study the role of NOS in PDT-induced photoreceptor degeneration
|She H,Nakazawa T,Matsubara A,Hisatomi T,Young TA,Michaud N,Connolly E,Hafezi-Moghadam A,Gragoudas ES,Miller JW||Investigative ophthalmology and visual science (48:2268)||2007|
Acetylcholine induces contractile and relaxant effects in canine nasal venous systems.
PA1-037 was used in immunohistochemistry to study how exogenous Ach affects different segments of the two nasal venous systems
|Wang M,Lung MA||The European respiratory journal (28:839)||2006|
Real-time observation of glomerular hemodynamic changes in diabetic rats: effects of insulin and ARB.
PA1-037 was used in immunohistochemistry to study real-time changes in glomerular hemodynamics in rats with diabetes mellitus in vivo using confocal laser scan microscopy
|Li B,Yao J,Kawamura K,Oyanagi-Tanaka Y,Hoshiyama M,Morioka T,Gejyo F,Uchiyama M,Oite T||Kidney international (66:1939)||2004|
High levels of exhaled nitric oxide (NO) and NO synthase III expression in lesional smooth muscle in lymphangioleiomyomatosis.
PA1-037 was used in immunohistochemistry to investigate the possible roles of NOSIII and NO in the abnormal smooth-muscle proliferation in LAM
|Dweik RA,Laskowski D,Ozkan M,Farver C,Erzurum SC||American journal of respiratory cell and molecular biology (24:414)||2001|
Cerivastatin prevents angiotensin II-induced renal injury independent of blood pressure- and cholesterol-lowering effects.
PA1-037 was used in immunohistochemistry to study the mechanism by which cerivastatin prevents angiotensin II-induced renal injury
|Park JK,Müller DN,Mervaala EM,Dechend R,Fiebeler A,Schmidt F,Bieringer M,Schäfer O,Lindschau C,Schneider W,Ganten D,Luft FC,Haller H||Kidney international (58:1420)||2000|
Olig1 is expressed in human oligodendrocytes during maturation and regeneration.
PA1-037 was used in immunocytochemistry to study the molecular mechanisms underlying the regeneration of primary human oligodendrocyte cultures isolated from epilepsy patients
|Othman A,Frim DM,Polak P,Vujicic S,Arnason BG,Boullerne AI||Glia (59:914)||2011|
Nitric oxide as a regulator of embryonic development.
PA1-037 was used in immunocytochemistry to study the role of NO in regulating embryonic development
|Gouge RC,Marshburn P,Gordon BE,Nunley W,Huet-Hudson YM||Biology of reproduction (58:875)||1998|