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Invitrogen

eNOS Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

3 Published Figures
46 References
View all (31) eNOS antibodies
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Product Details

Tested Applications
Dilution
Immunohistochemistry (Frozen) (IHC (F))
1:100
Immunohistochemistry (Paraffin) (IHC (P))
1:100
Western Blot (WB)
1:1,000
Published Applications
ChIP assay (ChIP)
See 1 publication below
Western Blot (WB)
See 14 publications below
Immunohistochemistry (IHC)
See 26 publications below
Immunohistochemistry (Paraffin) (IHC (P))
See 3 publications below
Immunocytochemistry (ICC)
See 2 publications below
Product Specifications
Species Reactivity
Bovine, Dog, Human, Mouse, Pig, Rat
Published species
Amphibian, Bovine, Cat, Dog, Fruit fly, Human, Mouse, Rabbit, Rat
Host / Isotype
Rabbit / IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues S(1179) L Q E R Q L R G A V P W A F D(1194) of human eNOS.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
RRID
AB_325774

Product Specific Information

PA1-037 detects endothelial nitric oxide synthase (eNOS) from bovine, canine, human, mouse, porcine and rat tissues as well as recombinant protein. This antibody does not detect human iNOS or rat nNOS.

PA1-037 has been successfully used in Western blot and immunohistochemistry (paraffin and frozen) procedures. By Western blot, this antibody detects an ~140 kDa protein representing eNOS from human endothelial cells. By Western blot PA1-037 also detects eNOS in rat lung, brain, and canine cardiac microsomes. Immunohistochemical staining of eNOS in human vascular endothelial cells with PA1-037 results in prominent staining of the epithelial cells.

The PA1-037 immunogen is a synthetic peptide corresponding to residues S(1179) L Q E R Q L R G A V P W A F D(1194) of human eNOS.

Target Information

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: cNOS; Constitutive NOS; e-NOS; EC-NOS; Endothelial Nitric Oxide Synthase; endothelial nitric oxide synthase 3; Endothelial NOS; eNOS; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; Nitric oxide synthase, endothelial; NOS Type III; NOSIII

Gene Aliases: 2310065A03Rik; BOS_4740; cNOS; EC-NOS; ECNOS; eNOS; NOS; Nos-3; NOS3; NOSIII

UniProt ID: (Pig) Q28969, (Human) P29474, (Mouse) P70313, (Rat) Q62600

Entrez Gene ID: (Bovine) 287024, (Pig) 397557, (Human) 4846, (Dog) 403784, (Mouse) 18127, (Rat) 24600

Function(s)
arginine catabolic process nitric oxide biosynthetic process mitochondrion organization blood coagulation positive regulation of guanylate cyclase activity negative regulation of extrinsic apoptotic signaling pathway via death domain receptors angiogenesis ovulation from ovarian follicle in utero embryonic development blood vessel remodeling regulation of sodium ion transport regulation of the force of heart contraction by chemical signal regulation of systemic arterial blood pressure by endothelin nitric oxide mediated signal transduction regulation of blood pressure negative regulation of cell proliferation response to heat negative regulation of platelet activation negative regulation of muscle hyperplasia smooth muscle hyperplasia removal of superoxide radicals lung development lipopolysaccharide-mediated signaling pathway response to fluid shear stress negative regulation of potassium ion transport endothelial cell migration cell redox homeostasis positive regulation of angiogenesis negative regulation of blood pressure positive regulation of vasodilation regulation of blood vessel size regulation of nitric-oxide synthase activity negative regulation of hydrolase activity negative regulation of calcium ion transport oxidation-reduction process signal transduction response to estradiol positive regulation of apoptotic process negative regulation of smooth muscle cell proliferation cellular response to mechanical stimulus cellular response to transforming growth factor beta stimulus response to hypoxia female pregnancy aging response to mechanical stimulus response to fructose response to metal ion response to lead ion response to organic cyclic compound response to activity response to lipopolysaccharide response to cytokine cellular response to heat response to statin response to drug response to amino acid response to peptide hormone response to ethanol response to axon injury response to corticosterone response to hyperoxia response to lipoprotein particle retina development in camera-type eye bone development cellular response to magnesium ion cellular response to cAMP cellular response to tumor necrosis factor cellular response to growth factor stimulus cellular response to organic cyclic compound response to candesartan response to peptide response to ketone response to salt
Process(es)
nitric-oxide synthase activity iron ion binding calmodulin binding FMN binding heme binding flavin adenine dinucleotide binding NADP binding actin monomer binding protein binding tetrahydrobiopterin binding arginine binding cadmium ion binding scaffold protein binding actin binding NADPH-hemoprotein reductase activity beta-catenin binding oxidoreductase activity cadherin binding metal ion binding nitric-oxide synthase binding Hsp90 protein binding

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