Immunofluorescent analysis of eNOS using eNOS Polyclonal Antibody (Product# PA3-031A ) shows staining in A2058 Cells. eNOS (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing eNOS (Product# PA3-031A ) at a dilution of 1:200 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
|Tested species reactivity||Bovine, Dog, Human, Mouse, Rat|
|Published species reactivity||Dog, Rat, Bovine, Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues P(599) Y N S S P R P E Q H K S Y K(613) C of bovine eNOS.|
|Storage buffer||whole serum diluted in PBS|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:250|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA3-031A detects endothelial Nitric Oxide Synthase (eNOS) from canine, bovine, human, mouse and rat tissues. This antibody does not detect brain NOS (bNOS) or inducible NOS (iNOS).
PA3-031A has been successfully used in Western blot, immunohistochemistry, immunofluorescence, and immunocytochemistry procedures. By Western blot, this antibody detects an approximately 140 kDa band representing eNOS from canine cardiac extracts. Immunohistochemical staining of eNOS in mouse cardiac tissue with PA3-031 results in the staining of the endothelium.
The PA3-031A immunogen is a synthetic peptide corresponding to residues P(599) Y N S S P R P E Q H K S Y K(613) C of bovine eNOS.
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
The elevation of intraocular pressure is associated with apoptosis and increased immunoreactivity for nitric oxide synthase in rat retina whereas the effectiveness of retina derived relaxing factor is unaffected.
PA3-031A was used in immunohistochemistry - paraffin section to investigate the association with apoptosis and increased immunoreactivity for nitric oxide synthase in rat retina causing an elevation of intraocular pressure and how the retina derived rela
|Tak¿r S,Gürel-Gürevin E,Toprak A,Demirci-Tansel C,Uyde¿-Do¿an BS||Experimental eye research (145:401)||2016|
Ameliorative effects of pentoxifylline on NOS induced by diabetes in rat kidney.
PA3-031A was used in immunohistochemistry - paraffin section to analyze NOS induced by diabetes in rat kidney and ameliorative effects of pentoxifylline
|Sönmez MF,Dündar M||Renal failure (38:605)||2016|
Nitric oxide synthase in diabetic rat testicular tissue and the effects of pentoxifylline therapy.
PA3-031A was used in immunohistochemistry and western blot to determine the effects of pentoxifylline therapy and nitric oxide synthase in diabetic rat testicular tissue
|Sönmez MF,K¿l¿ç E,Karabulut D,Çilenk K,Deligönül E,Dündar M||Systems biology in reproductive medicine (62:22)||2016|
GSPE is superior to NAC in the prevention of contrast-induced nephropathy: might this superiority be related to caspase 1 and calpain 1?
PA3-031A was used in immunohistochemistry to study the potential role of calpain-1 in the greater protective effect against contrast-induced nephropathy of grape seed proanthocyanidin extract as compared to N-acetyl cysteine
|Ulusoy S,Ozkan G,Mungan S,Orem A,Yulug E,Alkanat M,Yucesan FB||Life sciences (103:101)||2014|
Perspective on rhabdomyolysis-induced acute kidney injury and new treatment options.
PA3-031A was used in immunohistochemistry to study the protective effects of a grape seed proanthocyanidin extract in a rat model of acute kidney injury induced by rhabdomyolysis
|Ulusoy S,Ozkan G,Alkanat M,Mungan S,Yulu¿ E,Orem A||American journal of nephrology (38:368)||2013|
Immunohistochemical expression of nitric oxide synthase enzymes (iNOS, eNOS, nNOS) in the estrual and luteal phases of the sexual cycle in the cow oviduct.
PA3-031A was used in immunohistochemistry to study the bovine oviduct expression of iNOS, eNOS and nNOS across the sexual cycle
|Özen A,Ergün E,Özta¿ E,Ergün L,Özcan Z,Alabay B,Bayraktaro¿lu AG,Kürüm A,Erdo¿an E||Anatomia, histologia, embryologia (42:384)||2013|
Caloric restriction increases internal iliac artery and penil nitric oxide synthase expression in rat: comparison of aged and adult rats.
PA3-031A was used in immunohistochemistry to study the expression of eNOS and nNOS in the internal iliac artery and penis of rats subjected to caloric restriction
|Ozbek E,Simsek A,Ozbek M,Somay A||Archivio italiano di urologia, andrologia : organo ufficiale [di] Societa italiana di ecografia urologica e nefrologica (85:113)||2013|
Changes in renal vessels following the long-term administration of an angiotensin II receptor blocker in Zucker fatty rats.
PA3-031A was used in immunohistochemistry to identify how long term exposure to angiotensin II receptor blockers changes renal vessels
|Nakanishi K,Nagai Y,Akimoto T,Kato H,Yanakieva-Georgieva N,Ishikawa Y,Yoshihara K,Ito K,Yamanaka N,Oite T||Journal of the renin-angiotensin-aldosterone system : JRAAS (12:65)||2011|
The human placenta from heavy smokers: evaluation of vasoactive peptides by immunohistochemistry.
PA3-031A was used in immunohistochemistry to investigate the expression of e-NOS and endothelin-1 in human placenta from heavy smokers and non-smokers
|Clausen HV,Larsen LG,Jørgensen A,Bzorek M||APMIS : acta pathologica, microbiologica, et immunologica Scandinavica (115:22)||2007|
Warfarin reduces the incidence of osteonecrosis of the femoral head in spontaneously hypertensive rats.
PA3-031A was used in immunohistochemistry to study the effect of warfarin on the osteonecrosis of femoral head in spontaneously hypertensive rats
|Wada M,Kumagai K,Murata M,S-Yamashita Y,Shindo H||Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association (9:585)||2005|
GnRH agonist-suppressed expression of nitric oxide synthases and generation of peroxynitrite in adenomyosis.
PA3-031A was used in immunohistochemistry to investigate the role of gonadotrophin-releasing hormone agonists during nitric oxide synthase expression and peroxynitrite generation in adenomyosis
|Kamada Y,Nakatsuka M,Asagiri K,Noguchi S,Habara T,Takata M,Kudo T||Human reproduction (Oxford, England) (15:2512)||2000|
Experimental allergic encephalomyelitis in the rat is inhibited by aminoguanidine, an inhibitor of nitric oxide synthase.
PA3-031A was used in immunohistochemistry to investigate the role of nitric oxide production in the pathogenesis of rat experimental allergic encephalomyelitis
|Zhao W,Tilton RG,Corbett JA,McDaniel ML,Misko TP,Williamson JR,Cross AH,Hickey WF||Journal of neuroimmunology (64:123)||1996|
Sympathetic nervous system regulates bone marrow-derived cell egress through endothelial nitric oxide synthase activation: role in postischemic tissue remodeling.
PA3-031A was used in immunocytochemistry to investigate the mechanism of the regulation of bone marrow-derived cell egress by sympathetic nervous system
|Récalde A,Richart A,Guérin C,Cochain C,Zouggari Y,Yin KH,Vilar J,Drouet I,Lévy B,Varoquaux O,Silvestre JS||Arteriosclerosis, thrombosis, and vascular biology (32:643)||2012|
Expression of nitric oxide synthase and effect of substrate manipulation of the nitric oxide pathway in mouse ovarian follicles.
PA3-031A was used in immunocytochemistry to investigate the expression and role of nitric oxide synthase in mouse ovarian follicles
|Mitchell LM,Kennedy CR,Hartshorne GM||Human reproduction (Oxford, England) (19:30)||2004|
Decreased expression of myocardial eNOS and caveolin in dogs with hypertrophic cardiomyopathy.
PA3-031A was used in western blot to investigate the changes of eNOS and caveolin-1 and -3 in their respective expression level in a dog model of moderate, nonfailing, hypertrophic cardiomyopathy
|Piech A,Massart PE,Dessy C,Feron O,Havaux X,Morel N,Vanoverschelde JL,Donckier J,Balligand JL||American journal of physiology. Heart and circulatory physiology (282:H219)||2002|