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          • Primary Antibodies ›
          • eNOS Antibodies

          Invitrogen

          eNOS Polyclonal Antibody

          9 Published Figures
          29 References
          View all (44) eNOS antibodies

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          Cite eNOS Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (5)
          • Published Figures (9)
          eNOS Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          eNOS Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 14

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          eNOS Antibody (PA3-031A) in ICC/IF

          Immunofluorescent analysis of eNOS using eNOS Polyclonal Antibody (Product # PA3-031A) shows staining in A2058 Cells. eNOS (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing eNOS (Product # PA3-031A) at a dilution of 1:200 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552 for GAR, Product # 35... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          eNOS Antibody in Immunocytochemistry (ICC/IF)
          eNOS Antibody in Immunocytochemistry (ICC/IF)
          eNOS Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          eNOS Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          eNOS Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          eNOS Antibody in Immunohistochemistry (IHC)
          eNOS Antibody in Immunohistochemistry (IHC)
          eNOS Antibody in Western Blot (WB)
          eNOS Antibody in Immunocytochemistry (ICC/IF)
          eNOS Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          eNOS Antibody in Immunohistochemistry (IHC)
          eNOS Antibody in Immunohistochemistry (IHC)
          eNOS Antibody in Immunohistochemistry (IHC)
          eNOS Antibody in Immunohistochemistry (IHC)
          eNOS Polyclonal Antibody

          Product Details

          PA3-031A

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 3 publications 3 publications

          Immunohistochemistry (IHC)

          -
          View 16 publications 16 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:250
          View 4 publications 4 publications

          Immunocytochemistry (ICC/IF)

          1:250
          View 5 publications 5 publications

          Miscellaneous PubMed (Misc)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Bovine, Dog, Human, Mouse, Rat

          Published species

          Bovine, Dog, Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide corresponding to residues P(599) Y N S S P R P E Q H K S Y K(613) C of bovine eNOS.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          Conc. Not Determined

          Storage buffer

          whole serum, PBS

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2251447

          Product Specific Information

          PA3-031A detects endothelial Nitric Oxide Synthase (eNOS) from canine, bovine, human, mouse and rat tissues. This antibody does not detect brain NOS (bNOS) or inducible NOS (iNOS).

          PA3-031A has been successfully used in Western blot, immunohistochemistry, immunofluorescence, and immunocytochemistry procedures. By Western blot, this antibody detects an approximately 140 kDa band representing eNOS from canine cardiac extracts. Immunohistochemical staining of eNOS in mouse cardiac tissue with PA3-031 results in the staining of the endothelium.

          The PA3-031A immunogen is a synthetic peptide corresponding to residues P(599) Y N S S P R P E Q H K S Y K(613) C of bovine eNOS.

          Target Information

          Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: cNOS; Constitutive NOS; e-NOS; EC-NOS; endothelial nitric oxide synthase; endothelial nitric oxide synthase 3; Endothelial NOS; eNOS; nitric oxide synthase; Nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); Nitric oxide synthase, endothelial; NOS type III; NOSIII; OTTHUMP00000213183; unnamed protein product

          View more View less

          Gene Aliases: 2310065A03Rik; ECNOS; eNOS; NOS; Nos-3; NOS3

          View more View less

          UniProt ID: (Human) P29474, (Mouse) P70313, (Rat) Q62600

          View more View less

          Entrez Gene ID: (Bovine) 287024, (Dog) 403784, (Human) 4846, (Mouse) 18127, (Rat) 24600

          View more View less

          Function(s)
          nitric-oxide synthase activity calmodulin binding FMN binding heme binding metal ion binding flavin adenine dinucleotide binding NADP binding actin monomer binding protein binding superoxide-generating NADPH oxidase activity oxidoreductase activity tetrahydrobiopterin binding arginine binding cadmium ion binding scaffold protein binding actin binding NADPH-hemoprotein reductase activity iron ion binding beta-catenin binding cadherin binding nitric-oxide synthase binding Hsp90 protein binding oxidoreductase protein modifying enzyme
          Process(es)
          arginine catabolic process nitric oxide biosynthetic process mitochondrion organization blood coagulation positive regulation of guanylate cyclase activity oxidation-reduction process cellular response to laminar fluid shear stress negative regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of leukocyte cell-cell adhesion angiogenesis ovulation from ovarian follicle in utero embryonic development blood vessel remodeling regulation of sodium ion transport regulation of the force of heart contraction by chemical signal regulation of systemic arterial blood pressure by endothelin aortic valve morphogenesis pulmonary valve morphogenesis endocardial cushion morphogenesis potassium ion transport calcium ion transport nitric oxide mediated signal transduction blood circulation regulation of blood pressure negative regulation of cell proliferation response to heat response to hormone negative regulation of platelet activation positive regulation of gene expression negative regulation of muscle hyperplasia smooth muscle hyperplasia removal of superoxide radicals lung development regulation of neurological system process lipopolysaccharide-mediated signaling pathway response to lipopolysaccharide response to fluid shear stress vasodilation negative regulation of potassium ion transport positive regulation of blood vessel endothelial cell migration endothelial cell migration cell redox homeostasis positive regulation of Notch signaling pathway positive regulation of angiogenesis negative regulation of blood pressure tetrahydrobiopterin metabolic process nitric oxide metabolic process negative regulation of smooth muscle cell proliferation homeostasis of number of cells within a tissue establishment of localization in cell negative regulation of calcium ion transport ventricular septum morphogenesis negative regulation of biomineral tissue development regulation of blood vessel diameter signal transduction response to estradiol positive regulation of apoptotic process positive regulation of vasodilation regulation of blood vessel size negative regulation of hydrolase activity cellular response to mechanical stimulus cellular response to transforming growth factor beta stimulus response to hypoxia female pregnancy aging response to mechanical stimulus response to fructose response to metal ion response to lead ion response to organic cyclic compound response to activity response to cytokine cellular response to heat response to statin response to drug response to amino acid response to peptide hormone response to ethanol response to axon injury response to corticosterone response to hyperoxia response to lipoprotein particle retina development in camera-type eye bone development cellular response to magnesium ion cellular response to cAMP cellular response to tumor necrosis factor cellular response to growth factor stimulus cellular response to organic cyclic compound response to candesartan response to peptide response to ketone response to salt
          It has to be done as per old AB suggested Products section.

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