This Antibody was verified by KnockDown to ensure that the antibody binds to the antigen stated. View Details
PA5-28777 targets eRF1 in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-28777 immunogen is a synthetic peptide corresponding to a region within amino acids 374 and 437 of eRF1.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA; however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: D5S1995; ERF; ERF1; Eukaryotic peptide chain release factor subunit 1; Eukaryotic release factor 1; polypeptide chain release factor 1; Protein Cl1; RF1; sup45 (yeast omnipotent suppressor 45) homolog-like 1; SUP45L1; TB3-1
Gene Aliases: AI463371; D5S1995; D6Ertd109e; ERF; ERF1; ETF1; RF1; SUP45L1; TB3-1