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Invitrogen
hCG Monoclonal Antibody (SC2)
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FIGURE: 1 / 2
hCG Antibody (MIH9813) in WB
Western blot analysis of hCG was performed by loading the indicated amounts of purified hCG protein extracted from human urine, and 15 µL of PageRuler Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane (Product # 88518) using the G2 Fast Blotter (Product # 62288) and blocked with 5% Milk for at least 1 hour at room temperature. hCG was detected at ~32 & 22 kD using a anti hCG antibody (Product # MIH9813) at a concentration of 4 µg/mL in blocking buffer... View More
Product Details
MIH9813
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
SC2
Immunogen
Human Chorionic Gonadotropin
Conjugate
Form
Liquid
Concentration
5 mg/mL
Amount
1 mg
Purification
Ion-exchange chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The observed molecular weight of alpha-hCG and beta-hCG are approximately 20-22 kD & 27-32 kD respectively.
The molecular weight is higher than the predicted molecular weight due to post translational modification.
MIH9813 was formerly sold as a Seradyn product.
Target Information
Human chorionic gonadotropin (hCG) is a placental hormone that stimulates secretion of the pregnancy-sustaining steroid progesterone. hCG is a member of a family of glycoprotein hormones including pituitary hormones lutropin (luteinizing hormone;LH), follitropin (follicle-stimulating hormone; FSH) and thyrotropin (thyroid-stimulating hormone; TSH). Members of this family are all composed of disulfide-rich heterodimers with two dissimilar subunits namely alpha- and beta-subunits. The alpha subunit is common to all the members of the family within a given species. The beta subunits are distinct in each of the horomones and confer the receptor and biological specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Anterior pituitary glycoprotein hormones common subunit alpha; CG-alpha; CG-beta; CGA; Choriogonadotropin alpha chain; Choriogonadotropin subunit beta; Choriogonadotropin subunit beta 3; chorionic gonadotrophin chain beta; Chorionic gonadotrophin subunit alpha; chorionic gonadotropin beta 3 subunit; chorionic gonadotropin beta 5 subunit; chorionic gonadotropin beta 8 subunit; chorionic gonadotropin beta chain; chorionic gonadotropin beta subunit 3; chorionic gonadotropin beta subunit 5; chorionic gonadotropin beta subunit 8; Chorionic gonadotropin chain beta; chorionic gonadotropin human; chorionic gonadotropin, alpha polypeptide; chorionic gonadotropin, beta polypeptide; Follicle-stimulating hormone alpha chain; follicle-stimulating hormone alpha subunit; Follitropin alpha chain; FSH-alpha; Glycoprotein hormones alpha chain; human chorionic gonadotropin; LSH-alpha; Luteinizing hormone alpha chain; luteinizing hormone beta subunit; Lutropin alpha chain; Thyroid-stimulating hormone alpha chain; Thyrotropin alpha chain; TSH-alpha; unnamed protein product
Gene Aliases: CG-ALPHA; CGA; CGB; CGB3; CGB5; CGB7; CGB8; FSHA; GPA1; GPHa; GPHA1; HCG; hCGB; LHA; LHB; TSHA
UniProt ID: (Human) P01215, (Human) P0DN86
Entrez Gene ID: (Human) 1081, (Human) 1082, (Human) 93659, (Human) 94115
apoptotic process
signal transduction
G-protein coupled receptor signaling pathway
cell-cell signaling
female gamete generation
hormone-mediated signaling pathway
thyroid hormone generation
positive regulation of cell proliferation
gonad development
regulation of receptor activity
positive regulation of steroid biosynthetic process
positive regulation of cell migration
thyroid gland development
luteinizing hormone secretion
cellular response to hormone stimulus
organ growth
follicle-stimulating hormone signaling pathway
positive regulation of transcription from RNA polymerase II promoter
negative regulation of organ growth
follicle-stimulating hormone secretion
developmental growth
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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