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iNOS Polyclonal Antibody

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iNOS Polyclonal Antibody

Product Details

INOS-101AP

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

ELISA (ELISA)

1:10,000
-

Immunoprecipitation (IP)

1:50-1:250
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide taken within amino acid region 100-150 on human inducible nitric oxide synthase protein.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5-1.5 mg/mL

Purification

Affinity chromatography

Storage buffer

proprietary buffer, pH 7.4-7.8, with 0.5% BSA, 30% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

iNOS (Inducible Nitric oxide, NO, NOS) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. iNOS is expressed in liver and inducible by a combination of lipopolysaccharide and certain cytokines. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Diseases associated with iNOS dysfunction include achalasia and impotence.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: calcium-calmodulin independent nitric oxide synthase iNOS; HEP-NOS; Hepatocyte NOS; hepatocytes; inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; MAC-NOS; Macrophage NOS; nitric oxide synthase; nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase 2A (inducible, hepatocytes); Nitric oxide synthase, inducible; nitric oxide synthase, macrophage; nitric oxide synthase-inducible; NOS type II; NOS, type II; OTTMUSP00000000202; Peptidyl-cysteine S-nitrosylase NOS2; unnamed protein product

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Gene Aliases: HEP-NOS; i-NOS; INOS; Inosl; NOS; Nos-2; NOS-II; NOS2; NOS2A

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UniProt ID: (Human) P35228, (Mouse) P29477, (Rat) Q06518

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Entrez Gene ID: (Human) 4843, (Mouse) 18126, (Rat) 24599

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Function(s)
nitric-oxide synthase activity protein binding calmodulin binding FMN binding oxidoreductase activity heme binding tetrahydrobiopterin binding arginine binding protein homodimerization activity metal ion binding flavin adenine dinucleotide binding NADP binding actin binding NADPH-hemoprotein reductase activity receptor binding iron ion binding beta-catenin binding cAMP-dependent protein kinase regulator activity protein kinase binding cadherin binding nitric-oxide synthase binding Hsp90 protein binding amino acid binding oxidoreductase protein modifying enzyme
Process(es)
response to hypoxia positive regulation of leukocyte mediated cytotoxicity innate immune response in mucosa arginine catabolic process superoxide metabolic process nitric oxide biosynthetic process inflammatory response nitric oxide mediated signal transduction circadian rhythm response to bacterium response to hormone negative regulation of gene expression peptidyl-cysteine S-nitrosylation prostaglandin secretion response to lipopolysaccharide positive regulation of interleukin-6 production positive regulation of interleukin-8 production Fc-gamma receptor signaling pathway involved in phagocytosis regulation of cell proliferation negative regulation of protein catabolic process defense response to bacterium regulation of cellular respiration cell redox homeostasis negative regulation of blood pressure regulation of insulin secretion defense response to Gram-negative bacterium cellular response to lipopolysaccharide cellular response to interferon-gamma cellular response to xenobiotic stimulus regulation of cytokine production involved in inflammatory response ovulation from ovarian follicle positive regulation of guanylate cyclase activity cellular response to drug regulation of protein kinase activity positive regulation of vasodilation positive regulation of killing of cells of other organism oxidation-reduction process endothelial cell proliferation blood vessel remodeling signal transduction G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger aging regulation of heart contraction regulation of blood pressure response to mechanical stimulus response to activity glycoside metabolic process cGMP-mediated signaling response to estradiol response to tumor necrosis factor intracellular signal transduction response to immobilization stress wound healing positive regulation of apoptotic process retinoic acid receptor signaling pathway cellular response to lithium ion cellular response to vitamin D cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to redox state response to dexamethasone
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