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Fabgennix
iNOS Polyclonal Antibody
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FIGURE: 1 / 1
iNOS Antibody (INOS-112AP) in WB
Western blot of inducible Nitric oxide synthase antibody (iNOS-112AP) with: A) human Osteoblast cells B) cells treated to induce iNOS protein Cell extracts were made in SolObuffer with protease inhibitors and 50-60 µg protein was resolved on SDS-PAGE for Western blotting. Apparent MW of iNOS is 140 kDa. 1:500 antibody dilution in DiluOBuffer.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
INOS-112AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide taken within amino acid region 1064-1114 on human inducible nitric oxide synthase protein.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5-1.5 mg/mL
Purification
Affinity chromatography
Storage buffer
proprietary buffer, pH 7.4-7.8, with 30% glycerol, 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
iNOS (Inducible Nitric oxide, NO, NOS) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. iNOS is expressed in liver and inducible by a combination of lipopolysaccharide and certain cytokines. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Diseases associated with iNOS dysfunction include achalasia and impotence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: calcium-calmodulin independent nitric oxide synthase iNOS; hepatocyte NOS; hepatocytes; inducible NO synthase; inducible NOS; nitric oxide synthase; nitric oxide synthase 2, inducible; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase, macrophage; nitric oxide synthase-inducible; NOS, type II; OTTMUSP00000000202; peptidyl-cysteine S-nitrosylase NOS2; unnamed protein product
Gene Aliases: HEP-NOS; INOS; NOS; NOS2A
UniProt ID: (Human) P35228
Entrez Gene ID: (Human) 4843
positive regulation of leukocyte mediated cytotoxicity
innate immune response in mucosa
arginine catabolic process
superoxide metabolic process
nitric oxide biosynthetic process
inflammatory response
circadian rhythm
response to bacterium
response to hormone
negative regulation of gene expression
peptidyl-cysteine S-nitrosylation
prostaglandin secretion
response to lipopolysaccharide
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
nitric oxide-cGMP-mediated signaling pathway
Fc-gamma receptor signaling pathway involved in phagocytosis
negative regulation of protein catabolic process
defense response to bacterium
regulation of cellular respiration
cell redox homeostasis
negative regulation of blood pressure
regulation of insulin secretion
defense response to Gram-negative bacterium
regulation of cytokine production involved in inflammatory response
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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