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      View (28) other iNOS antibodies

      Invitrogen

      iNOS Polyclonal Antibody

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      Antibody target was verified by Cell Treatment to ensure the antibody binds to the antigen stated. View details.

      References Icon 36 References
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      Catalog #:PA3-030A

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      Product Details

      TESTED APPLICATIONS DILUTION
      Immunocytochemistry (ICC) 1:250
      Immunofluorescence (IF) 1:250
      Immunohistochemistry (Frozen) (IHC (F)) 1:500
      Immunohistochemistry (Paraffin) (IHC (P)) 1:200
      Western Blot (WB) 1:2,000
      PUBLISHED APPLICATIONS
      Western Blot (WB) See 8 publications below
      Immunohistochemistry (Paraffin) (IHC (P)) See 9 publications below
      Immunohistochemistry (IHC) See 17 publications below
      Miscellaneous PubMed (MISC) See 1 publications below
      Immunocytochemistry (ICC) See 1 publications below
      Tested Species reactivity Human, Mouse
      Published species reactivity Rat , Sheep , Cat , Mouse , Human
      Host / Isotype Rabbit / IgG
      Class Polyclonal
      Type Antibody
      Immunogen Synthetic peptide corresponding to residues C K(1131) K G S A L E E P K A T R L(1144) of mouse macrophage NOS.
      Conjugate Unconjugated
      Form Liquid
      Storage buffer whole serum diluted in PBS
      Contains 0.05% sodium azide
      Storage conditions -20° C, Avoid Freeze/Thaw Cycles
      RRID AB_2152737

      Product Specific Information

      PA3-030A detects inducible nitric oxide synthase (iNOS) from human and mouse tissues. This antibody does not detect endothelial NOS (eNOS) or brain NOS (bNOS).

      PA3-030A has been successfully used in Western blot and immunohistochemistry (paraffin and frozen) procedures. By Western blot, this antibody detects an ~130 kDa protein representing iNOS in induced RAW 264.7 cell extracts.

      The PA3-030A immunogen is a synthetic peptide corresponding to residues C K(1131) K G S A L E E P K A T R L(1144) of mouse macrophage NOS.



      Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.

      Background/Target Information

      Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.

      iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time.

      For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

      References (36)

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      Bioinformatics

      Protein Aliases: HEP-NOS; Hepatocyte NOS; Inducible Nitric Oxide Synthase; Inducible NO synthase; Inducible NOS; MAC-NOS; Macrophage NOS; nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase 2A (inducible, hepatocytes); Nitric oxide synthase, inducible; nitric oxide synthase, macrophage; NOS Type II; NOS, type II; Peptidyl-cysteine S-nitrosylase NOS2

      Gene Aliases: HEP-NOS; i-NOS; INOS; Inosl; NOS; Nos-2; NOS-II; NOS2; NOS2A

      UniProt ID: (Human) P35228, (Mouse) P29477

      Entrez Gene ID: (Human) 4843, (Mouse) 18126

      Function(s) Blue Arrow Icon
      response to hypoxia positive regulation of leukocyte mediated cytotoxicity innate immune response in mucosa arginine catabolic process superoxide metabolic process nitric oxide biosynthetic process nitric oxide mediated signal transduction circadian rhythm response to bacterium negative regulation of gene expression peptidyl-cysteine S-nitrosylation positive regulation of guanylate cyclase activity prostaglandin secretion cellular response to drug regulation of cell proliferation negative regulation of protein catabolic process defense response to bacterium regulation of cellular respiration cell redox homeostasis negative regulation of blood pressure positive regulation of vasodilation regulation of insulin secretion defense response to Gram-negative bacterium positive regulation of killing of cells of other organism oxidation-reduction process cellular response to lipopolysaccharide cellular response to interferon-gamma interleukin-6 secretion interleukin-8 secretion regulation of cytokine production involved in inflammatory response ovulation from ovarian follicle inflammatory response response to lipopolysaccharide regulation of protein kinase activity
      Process(es) Blue Arrow Icon
      nitric-oxide synthase activity
       receptor binding
       protein binding
       calmodulin binding
       FMN binding
       heme binding
       tetrahydrobiopterin binding
       arginine binding
       protein homodimerization activity
       metal ion binding
       flavin adenine dinucleotide binding
       NADP binding
       actin binding
       NADPH-hemoprotein reductase activity
       iron ion binding
       beta-catenin binding
       cAMP-dependent protein kinase regulator activity
       oxidoreductase activity
       protein kinase binding
       cadherin binding
       nitric-oxide synthase binding
       Hsp90 protein binding

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