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iNOS Polyclonal Antibody
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FIGURE: 1 / 51
iNOS Antibody (PA3-030A) in ICC/IF
Immunofluorescence analysis of iNOS was performed using 70% confluent log phase A549 cells treated with a combination of TNF-α (5 ng/mL), IFNγ (50 U/mL) and IL-1β (50 U/mL) for 18 h. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with iNOS Rabbit Polyclonal Antibody (Product # PA3-030A) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-R... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA3-030A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Cat,
Dog,
Human,
Mouse,
Rat,
Sheep
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues C K(1131) K G S A L E E P K A T R L(1144) of mouse macrophage NOS.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
PBS, whole serum
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA3-030A detects inducible nitric oxide synthase (iNOS) from human and mouse tissues. This antibody does not detect endothelial NOS (eNOS) or brain NOS (bNOS).
PA3-030A has been successfully used in Western blot and immunohistochemistry (paraffin and frozen) procedures. By Western blot, this antibody detects an ~130 kDa protein representing iNOS in induced RAW 264.7 cell extracts.
The PA3-030A immunogen is a synthetic peptide corresponding to residues C K(1131) K G S A L E E P K A T R L(1144) of mouse macrophage NOS.
PA3-030A can be used with blocking peptide PEP-008.
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.
Target Information
iNOS (Inducible Nitric oxide, NO, NOS) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. iNOS is expressed in liver and inducible by a combination of lipopolysaccharide and certain cytokines. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Diseases associated with iNOS dysfunction include achalasia and impotence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: calcium-calmodulin independent nitric oxide synthase iNOS; hepatocyte NOS; hepatocytes; inducible nitric oxide synthase; inducible NO synthase; inducible NOS; macrophage NOS; nitric oxide synthase; nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase, macrophage; nitric oxide synthase-inducible; NOS type II; NOS, type II; OTTMUSP00000000202; peptidyl-cysteine S-nitrosylase NOS2; unnamed protein product
Gene Aliases: HEP-NOS; i-NOS; INOS; MAC-NOS; NOS; Nos-2; NOS-II; NOS2A
UniProt ID: (Human) P35228, (Mouse) P29477
Entrez Gene ID: (Human) 4843, (Mouse) 18126
nitric-oxide synthase activity
protein binding
calmodulin binding
FMN binding
oxidoreductase activity
heme binding
tetrahydrobiopterin binding
arginine binding
protein homodimerization activity
flavin adenine dinucleotide binding
NADP binding
actin binding
beta-catenin binding
cAMP-dependent protein kinase regulator activity
protein kinase binding
identical protein binding
cadherin binding
nitric-oxide synthase binding
Hsp90 protein binding
positive regulation of leukocyte mediated cytotoxicity
innate immune response in mucosa
arginine catabolic process
superoxide metabolic process
nitric oxide biosynthetic process
inflammatory response
circadian rhythm
response to bacterium
response to hormone
negative regulation of gene expression
peptidyl-cysteine S-nitrosylation
prostaglandin secretion
response to lipopolysaccharide
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
nitric oxide-cGMP-mediated signaling pathway
Fc-gamma receptor signaling pathway involved in phagocytosis
negative regulation of protein catabolic process
defense response to bacterium
regulation of cellular respiration
cell redox homeostasis
negative regulation of blood pressure
regulation of insulin secretion
defense response to Gram-negative bacterium
regulation of cytokine production involved in inflammatory response
response to hypoxia
blood vessel remodeling
G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger
regulation of blood pressure
response to tumor necrosis factor
intracellular signal transduction
regulation of cell proliferation
positive regulation of apoptotic process
cellular response to lipopolysaccharide
cellular response to cytokine stimulus
cellular response to interferon-gamma
cellular response to xenobiotic stimulus
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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