Invitrogen

mGluR2 Polyclonal Antibody

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Datasheet

Protocols

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mGluR2 Antibody in Immunocytochemistry (ICC/IF) — HEK 293 cells were grown on poly-L-lysine-coated coverslips overnight. Cells were then transiently transfected with mGLUR2. After 24 h, cells were fixed with 4% paraformaldehyde and 0.2% picric acid in phosphate buffer (pH 6.9) for 30 min at room temperature and washed several times with phosphate buffer. Specimens were permeabilized with ice-cold methanol and then incubated with Anti-mGLUR2 Rabbit Polyclonal Antibody (Product # 720275 at a concentration of 2 µg/mL). Cells were then incubated with Alexa488-conjugated secondary antibody for 2 h at room temperature, mounted and examined using a laser scanning confocal microscope. Note, strong staining of transfected cells and no staining of adjacent non-transfected cells.

mGluR2 Antibody in Immunohistochemistry (IHC) — Sections of human cerebral cortex were dewaxed, microwaved in citric acid and incubated with Anti-mGLUR2 Rabbit Polyclonal Antibody (Product # 720275 at a concentration of 2 µg/mL). Sections were then sequentially treated with biotinylated anti-rabbit IgG and AB solution. Sections were then developed in AEC and lightly counterstained with hematoxylin. Inset, for adsorption controls the antibody was incubated with 10 µg/mL of the peptide used for immunizations (+ Peptide). Note, staining of neuronal somata and dendrites is completely neutralized by preincubation of the antibody with its immunizing peptide.

mGluR2 Antibody in Western Blot (WB) — HEK 293 cells were transiently transfected with mGLUR2 or mGLUR4. After 48 h, cells were lysed in detergent buffer and glycoproteins were enriched using wheat germ lectin agarose beads. Receptors were eluted from the beads using SDS sample buffer for 20 min at 45 °C. Extracts were separated on 7.5% SDS-polyacrylamide gels and blotted onto PVDF membranes. Membranes were then incubated with Anti-mGLUR2 Rabbit Polyclonal Antibody (Product # 720275 at a concentration of 2 µg/mL). A 120 kDa (monomer), 240 kDa (dimer) band corresponding to mGlur2 was observed.

Product Details

720275

Applications	Tested Dilution	Publications
Western Blot (WB)	1-2 µg/mL	-
Immunohistochemistry (IHC)	2 µg/mL	-
Immunocytochemistry (ICC/IF)	1-2 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide corresponding to Human GRM2 (aa 861-872) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 30% glycerol, 0.1% BSA
Contains	0.09% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2633230

Product Specific Information

These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.

This antibody is predicted to react with Monkey, Rabbit and Bovine.

Target Information

Glutamate is the main excitatory neurotransmitter in the brain. For many years it had been considered to act only on the ligand-gated receptor channels-termed NMDA, AMPA and kainite receptors that are involved in the fast excitatory synaptic transmission. Recently, glutamate has been shown to regulate enzymes producing second messengers via specific receptors coupled to G-proteins. These receptors are called metabotropic glutamate receptors. In expression systems, Group-I receptors stimulate phospholipase C as revealed by an increase in phosphoinositide turnover and calcium release from internal stores. Group-II and -III receptors are coupled to the inhibition of adenylyl cyclase. The Group-I receptors include mGluR1a and mGluR5. The Group-II receptors in include mGluR2 and mGluR3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: glutamate receptor; glutamate receptor homolog; glutamate receptor, metabotropic 2; M mGlu2; Metabotropic; Metabotropic glutamate receptor 2; mGluR2

Gene Aliases: GLUR2; GPRC1B; GRM2; mGlu2; MGLUR2

UniProt ID: (Human) Q14416

Entrez Gene ID: (Human) 2912

Molecular Function: G-protein coupled receptor

Function(s)

negative regulation of adenylate cyclase activity adenylate cyclase-inhibiting G-protein coupled glutamate receptor signaling pathway chemical synaptic transmission glutamate secretion regulation of synaptic transmission, glutamatergic

Process(es)

group II metabotropic glutamate receptor activity G-protein coupled receptor activity calcium channel regulator activity protein binding glutamate receptor activity

mGluR2 Polyclonal Antibody

Product Details

720275

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

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