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Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:2500-1:5000 | - |
Immunocytochemistry (ICC/IF) |
1:50-1:200 | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Non-human primate |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Recombinant protein corresponding to amino acids 2447-2549 of human mTOR expressed in E. coli. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Protein G |
Storage buffer |
PBS with 30% glycerol, 1mg/mL BSA |
Contains |
0.05% sodium azide |
Storage conditions |
-20°C |
RRID |
AB_2539920 |
Western blot analysis of PA1-188 detects mTOR at ~250 kDa in human and non-human primate cells, slightly below the theoretical MW of ~280kDa. Using a Tris-Acetate SDS gel for high molecular weight protein separation, and a nitrocellulose membrane for protein transfer are necessary for optimal WB results.
Immunofluorescence analysis shows cytoplasmic and some nuclear staining of mTOR in human B3 and U-2 OS cells.
Mammalian target of rapamycin (mTOR) is a serine/threonine kinase that plays a key role in cell growth, cell proliferation, and protein synthesis. mTOR mediates phosphoinositide 3-kinase and Akt/PKB signaling, resulting in phosphorylation of 4EBP1, and initiation of mRNA translation. A second pathway involves regulation of ribosomal S6 kinase, which affects ribosome biogenesis and translation elongation. Mutations affecting the gene results in Smith-Kingsmore syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein; FKBP12-rapamycin complex-associated protein; FKBP12-rapamycin complex-associated protein 1; m-TOR; Mammalian target of rapamycin; Mechanistic target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); mTOR; mTORC1; Rapamycin and FKBP12 target 1; rapamycin associated protein FRAP2; Rapamycin target protein 1; Serine/threonine-protein kinase mTOR
Gene Aliases: FRAP; FRAP1; FRAP2; MTOR; RAFT1; RAPT1; SKS
UniProt ID: (Human) P42345
Entrez Gene ID: (Human) 2475
Molecular Function:
non-receptor serine/threonine protein kinase
protein modifying enzyme
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