Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:100-1:1,000 | View 2 publications 2 publications |
Immunocytochemistry (ICC/IF) |
1:50-1:200 | - |
Immunoprecipitation (IP) |
2 µg | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Non-human primate, Rat |
Published species |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Immunogen corresponds to amino acids 1223-1290 of human mTOR. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Protein G |
Storage buffer |
PBS with 1mg/mL BSA, 30% glycerol |
Contains |
0.05% sodium azide |
Storage conditions |
-20°C |
RRID |
AB_325371 |
3-8% Tris-Acetate Gel is recommended for Western blot.
PA1-518 detects a predominant band at ~290kD corresponding to mTOR. Other lower MW nonspecific bands of unknown identity were also detected at ~65kD and ~35kD in some cell lysates.
Mammalian target of rapamycin (mTOR) is a serine/threonine kinase that plays a key role in cell growth, cell proliferation, and protein synthesis. mTOR mediates phosphoinositide 3-kinase and Akt/PKB signaling, resulting in phosphorylation of 4EBP1, and initiation of mRNA translation. A second pathway involves regulation of ribosomal S6 kinase, which affects ribosome biogenesis and translation elongation. Mutations affecting the gene results in Smith-Kingsmore syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: angiopoietin-like factor CDT6; FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein; FKBP-rapamycin associated protein (FRAP); FKBP-rapamycin-associated protein FRAP; FKBP12-rapamycin complex-associated protein; FKBP12-rapamycin complex-associated protein 1; m-TOR; Mammalian target of rapamycin; Mechanistic target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); mTOR; mTORC1; Rapamycin and FKBP12 target 1; rapamycin and FKBP12 target-1 protein; rapamycin associated protein FRAP2; Rapamycin target protein 1; RAPT1; Serine/threonine-protein kinase mTOR
Gene Aliases: 2610315D21Rik; AI327068; flat; FRAP; FRAP1; FRAP2; MTOR; RAFT1; RAPT1; SKS
UniProt ID: (Human) P42345, (Mouse) Q9JLN9, (Rat) P42346
Entrez Gene ID: (Human) 2475, (Mouse) 56717, (Rat) 56718
Molecular Function:
non-receptor serine/threonine protein kinase
protein modifying enzyme
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