|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 2386 and 2549 of mTOR (Uniprot ID#P42345)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% ProClin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay-dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34663 targets mTOR in IHC (P), IF, IP, ChIP, and WB applications and shows reactivity with Human, Mouse, and Rat samples.
The PA5-34663 immunogen is recombinant fragment corresponding to a region within amino acids 2386 and 2549 of mTOR (Uniprot ID#P42345).
Mechanistic target of rapamycin (mTOR) is a serine/threonine protein kinase which is a central regulator of cellular metabolism, growth, and survival in response to hormones, growth factors, nutrients, energy, and stress signals. mTOR directly or indirectly regulates the phosphorylation of at least 800 proteins, and functions as part of 2 structurally and functionally distinct signaling complexes, mTORC1 and mTORC2 (mTOR complex 1 and 2). Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. As part of the mTORC2 complex, mTOR regulates cellular processes including survival and organization of the cytoskeleton. It plays a critical role in the phosphorylation at Ser-473 of AKT1. Mechanistic target of rapamycin complex 1 (mTORC1) contains mTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. mTORC2 contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1 and DEPTOR. Due to its central role in celliular signaling, mTOR is associated with a vast array of diseases, including many cancers, inflammation, cellular growth, and metabolic disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.