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|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Rat, Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A recombinant protein consisting of 195 amino acids from the N-terminal of rat nNOS protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Paraffin) (IHC (P))||See 3 publications below|
|Immunohistochemistry (IHC)||See 6 publications below|
|Western Blot (WB)||See 3 publications below|
|Immunofluorescence (IF)||See 5 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding omain. bNOS and eNOS share approximately 50% sequence homology and their enzymatic activity depends on binding to the calcium/calmodulin complex. Increases in intracellular calcium lead to the production of low levels of NO over a short time. bNOS is found in neurons, peripheral nerve cells, macula densa, and pancreatic islet cells. Alternate splicing specifically regulates bNOS in striated muscle. The translated protein, bNOS mu, is 34 amino acid residues larger than bNOS from brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Homeostatic sleep pressure is the primary factor for activation of cortical nNOS/NK1 neurons.
61-7000 was used in immunohistochemistry - paraffin section to study the effects of sleep and wake duration on cortical nNOS/NK1 neuron activation.
|Dittrich L,Morairty SR,Warrier DR,Kilduff TS||Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (40:632)||2015|
Megaesophagus in a line of transgenic rats: a model of achalasia.
61-7000 was used in immunohistochemistry - paraffin section to characterize the use of Pvrl3-Cre transgenic rats to use as an animal model of megaesophagus and achalasia.
|Pang J,Borjeson TM,Muthupalani S,Ducore RM,Carr CA,Feng Y,Sullivan MP,Cristofaro V,Luo J,Lindstrom JM,Fox JG||Veterinary pathology (51:1187)||2014|
Upregulation of nitric oxide synthase in mice with severe hypoxia-induced pulmonary hypertension.
61-7000 was used in immunohistochemistry - paraffin section to determine how hypoxia alters NOS expression in wild-type mice.
|Fagan KA,Morrissey B,Fouty BW,Sato K,Harral JW,Morris KG,Hoedt-Miller M,Vidmar S,McMurtry IF,Rodman DM||Respiratory research (2:306)||2001|
Characterization of axons expressing the artemin receptor in the female rat urinary bladder: a comparison with other major neuronal populations.
61-7000 was used in immunohistochemistry to investigate the role of artemin and its receptor in bladder physiology and pathophysiology
|Forrest SL,Osborne PB,Keast JR||The Journal of comparative neurology (522:3900)||2014|
Multiple embryonic origins of nitric oxide synthase-expressing GABAergic neurons of the neocortex.
61-7000 was used in immunohistochemistry to study neuronal NOS-expressing neurons in embryos and adult mice.
|Magno L,Oliveira MG,Mucha M,Rubin AN,Kessaris N||Frontiers in neural circuits (6:null)||2012|
|Mouse||Not Cited||Nitric oxide signaling modulates synaptic transmission during early postnatal development.||Cserép C,Szonyi A,Veres JM,Németh B,Szabadits E,de Vente J,Hájos N,Freund TF,Nyiri G||Cerebral cortex (New York, N.Y. : 1991) (21:2065)||2011|
Distribution of soluble guanylyl cyclase in rat retina.
61-7000 was used in immunohistochemistry to examine soluble guanylyl cyclase in rat retina
|Ding JD,Weinberg RJ||The Journal of comparative neurology (500:734)||2007|
Expression of soluble guanylyl cyclase in rat cerebral cortex during postnatal development.
61-7000 was used in immunohistochemistry to test if the NO/cGMP pathway contributes to neural development in vivo.
|Ding JD,Burette A,Weinberg RJ||The Journal of comparative neurology (485:255)||2005|
Distribution of soluble guanylyl cyclase in the rat brain.
61-7000 was used in immunohistochemistry to examine the distribution of sGC subunits in the brain of adult rats.
|Ding JD,Burette A,Nedvetsky PI,Schmidt HH,Weinberg RJ||The Journal of comparative neurology (472:437)||2004|
|Mouse||1:1000||Tetrahydrobiopterin deficiency induces gastroparesis in newborn mice.||Welsh C,Enomoto M,Pan J,Shifrin Y,Belik J||American journal of physiology. Gastrointestinal and liver physiology (305:G47)||2013|
Fatigue and muscle atrophy in a mouse model of myasthenia gravis is paralleled by loss of sarcolemmal nNOS.
61-7000 was used in western blot to assess the role of nNOS in chronic muscle fatigue and muscle atrophy.
|Meinen S,Lin S,Rüegg MA,Punga AR||PloS one (7:null)||2012|
|Mouse||Not Cited||Divergent contractile and structural responses of the murine PKC-epsilon null pulmonary circulation to chronic hypoxia.||Littler CM,Wehling CA,Wick MJ,Fagan KA,Cool CD,Messing RO,Dempsey EC||American journal of physiology. Lung cellular and molecular physiology (289:L1083)||2005|
|Mouse||Not Cited||Neurturin regulates postnatal differentiation of parasympathetic pelvic ganglion neurons, initial axonal projections, and maintenance of terminal fields in male urogenital organs.||Yan H,Keast JR||The Journal of comparative neurology (507:1169)||2008|
Expression of receptors for glial cell line-derived neurotrophic factor family ligands in sacral spinal cord reveals separate targets of pelvic afferent fibers.
61-7000 was used in immunohistochemistry to localize the receptors for glial cell line-derived neurotrophic factor family ligands in sacral spinal cord and their roles in neuronal plasticity
|Forrest SL,Keast JR||The Journal of comparative neurology (506:989)||2008|
|Rat||Not Cited||Distribution of soluble guanylyl cyclase in rat retina.||Ding JD,Weinberg RJ||The Journal of comparative neurology (502:734)||2007|
|Mouse||Not Cited||High concentration of glucose inhibits glomerular endothelial eNOS through a PKC mechanism.||Chu S,Bohlen HG||American journal of physiology. Renal physiology (287:F384)||2004|
|Mouse||Not Cited||Stimulation of calcineurin signaling attenuates the dystrophic pathology in mdx mice.||Chakkalakal JV,Harrison MA,Carbonetto S,Chin E,Michel RN,Jasmin BJ||Human molecular genetics (13:379)||2004|
|Mouse||1:200||Layer positioning of late-born cortical interneurons is dependent on Reelin but not p35 signaling.||Hammond V,So E,Gunnersen J,Valcanis H,Kalloniatis M,Tan SS||The Journal of neuroscience : the official journal of the Society for Neuroscience (26:1646)||2006|
|Human||Not Cited||PSD-95 assembles a ternary complex with the N-methyl-D-aspartic acid receptor and a bivalent neuronal NO synthase PDZ domain.||Christopherson KS,Hillier BJ,Lim WA,Bredt DS||The Journal of biological chemistry (274:27467)||1999|
brain; constitutive NOS; IHPS1; N-NOS; NC-NOS; neuronal NOS; nitric oxidase synthase; nitric oxide synthase 1 (neuronal); nitric oxide synthase, brain; nNOS; NOS; NOS type I; NOS1; peptidyl-cysteine S-nitrosylase NOS1
2310005C01Rik; Bnos; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; NOS-I; Nos1