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Invitrogen
nNOS Polyclonal Antibody
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
61-7000
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A recombinant protein consisting of 195 amino acids from the N-terminus of rat nNOS protein.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.25 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with the ~160 kDa nNOS protein and does not exhibit any cross-reactivity with the related eNOS or iNOS proteins. During development reactivity was confirmed with a ~160 kD band on western blots of rat and mouse brain tissue lysates (20 µg). For use in immunohistochemistry with paraffin embedded tissues some background staining was evident.
Target Information
NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: bNOS1; Brain NOS; constitutive NOS; N-NOS; NC-NOS; neuronal nitric oxide synthase; neuronal NOS; nitric oxidase synthase; nitric oxide synthase 1, neuronal; nitric oxide synthase, brain; nNOS; nNOS mu; NOS Type 1; NOS type I; peptidyl-cysteine S-nitrosylase NOS1; Phospho-bNOS; Phospho-Brain NOS; unnamed protein product
Gene Aliases: 2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; NOS-I
UniProt ID: (Mouse) Q9Z0J4, (Rat) P29476
Entrez Gene ID: (Mouse) 18125, (Rat) 24598
nitric-oxide synthase activity
protein binding
calmodulin binding
zinc ion binding
FMN binding
oxidoreductase activity
sodium channel regulator activity
enzyme binding
heme binding
peptidyl-cysteine S-nitrosylase activity
identical protein binding
ion channel binding
cadmium ion binding
calcium-dependent protein binding
flavin adenine dinucleotide binding
NADP binding
ATPase binding
phosphoprotein binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
NADPH binding
scaffold protein binding
response to hypoxia
regulation of the force of heart contraction
regulation of sodium ion transport
system process
arginine catabolic process
nitric oxide biosynthetic process
striated muscle contraction
cell communication
negative regulation of cell proliferation
response to heat
response to hormone
peptidyl-cysteine S-nitrosylation
signaling
response to lipopolysaccharide
multicellular organismal response to stress
negative regulation of iron ion transmembrane transport
nitric oxide-cGMP-mediated signaling pathway
xenobiotic catabolic process
vasodilation
negative regulation of apoptotic process
negative regulation of potassium ion transport
response to peptide hormone
negative regulation of neuron apoptotic process
positive regulation of neuron apoptotic process
response to estrogen
establishment of protein localization
positive regulation of G-protein coupled receptor protein signaling pathway
negative regulation of blood pressure
positive regulation of transcription, DNA-templated
negative regulation of vasoconstriction
positive regulation of transcription from RNA polymerase II promoter
negative regulation of insulin secretion
behavioral response to cocaine
regulation of neurogenesis
negative regulation of hydrolase activity
negative regulation of cytosolic calcium ion concentration
negative regulation of serotonin uptake
negative regulation of calcium ion transport
regulation of postsynaptic membrane potential
negative regulation of hepatic stellate cell contraction
cellular response to mechanical stimulus
cellular response to growth factor stimulus
cellular response to epinephrine stimulus
positive regulation of adrenergic receptor signaling pathway
positive regulation of the force of heart contraction
synaptic signaling by nitric oxide
positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway
positive regulation of long-term synaptic potentiation
positive regulation of sodium ion transmembrane transport
female pregnancy
response to mechanical stimulus
response to lead ion
response to activity
response to nutrient levels
response to vitamin E
response to vitamin B3
response to nicotine
response to decreased oxygen levels
regulation of apoptotic process
negative regulation of ion transport
response to ethanol
nitric oxide metabolic process
response to nitric oxide
regulation of blood circulation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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