|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Rat, Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A recombinant protein consisting of 195 amino acids from the N-terminal of rat nNOS protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 10 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 6 publications below|
|Miscellaneous PubMed (MISC)||See 3 publications below|
|Western Blot (WB)||See 6 publications below|
|Immunofluorescence (IF)||See 5 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding omain. bNOS and eNOS share approximately 50% sequence homology and their enzymatic activity depends on binding to the calcium/calmodulin complex. Increases in intracellular calcium lead to the production of low levels of NO over a short time. bNOS is found in neurons, peripheral nerve cells, macula densa, and pancreatic islet cells. Alternate splicing specifically regulates bNOS in striated muscle. The translated protein, bNOS mu, is 34 amino acid residues larger than bNOS from brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Glucagon-Like Peptide-1 Excites Firing and Increases GABAergic Miniature Postsynaptic Currents (mPSCs) in Gonadotropin-Releasing Hormone (GnRH) Neurons of the Male Mice via Activation of Nitric Oxide (NO) and Suppression of Endocannabinoid Signaling Pathways.
61-7000 was used in immunohistochemistry describe the pathways regulated by glucagon-like peptide-1
|Farkas I,Vastagh C,Farkas E,Bálint F,Skrapits K,Hrabovszky E,Fekete C,Liposits Z||Frontiers in cellular neuroscience (10:null)||2016|
Characterization of axons expressing the artemin receptor in the female rat urinary bladder: a comparison with other major neuronal populations.
61-7000 was used in immunohistochemistry to investigate the role of artemin and its receptor in bladder physiology and pathophysiology
|Forrest SL,Osborne PB,Keast JR||The Journal of comparative neurology (522:3900)||2014|
Multiple embryonic origins of nitric oxide synthase-expressing GABAergic neurons of the neocortex.
61-7000 was used in immunohistochemistry to study neuronal NOS-expressing neurons in embryos and adult mice.
|Magno L,Oliveira MG,Mucha M,Rubin AN,Kessaris N||Frontiers in neural circuits (6:null)||2012|
Expression of the neuregulin receptor ErbB4 in the brain of the rhesus monkey (Macaca mulatta).
61-7000 was used in immunohistochemistry to determine the localization of the ErbB4 protein in the cerebrum of primates
|Neddens J,Buonanno A||PloS one (6:null)||2011|
|Mouse||Not Cited||Nitric oxide signaling modulates synaptic transmission during early postnatal development.||Cserép C,Szonyi A,Veres JM,Németh B,Szabadits E,de Vente J,Hájos N,Freund TF,Nyiri G||Cerebral cortex (New York, N.Y. : 1991) (21:2065)||2011|
Distribution of soluble guanylyl cyclase in rat retina.
61-7000 was used in immunohistochemistry to examine soluble guanylyl cyclase in rat retina
|Ding JD,Weinberg RJ||The Journal of comparative neurology (500:734)||2007|
Separate development of nitric oxide synthase- and vasoactive intestinal polypeptide-immunoreactive nerves arising from the vertebral artery in the rat.
61-7000 was used in immunohistochemistry to study the development of nitric oxide synthase- and vasoactive intestinal polypeptide-immunoreactive nerves in rats
|Ando K,Yoshida S,Yamauchi H,Kubo S,Kusaba H||The Journal of veterinary medical science (68:125)||2006|
Memory consolidation of Pavlovian fear conditioning requires nitric oxide signaling in the lateral amygdala.
61-7000 was used in immunohistochemistry to assess the role of NO signaling in Pavlovian fear conditioning
|Schafe GE,Bauer EP,Rosis S,Farb CR,Rodrigues SM,LeDoux JE||The European journal of neuroscience (22:201)||2005|
Expression of soluble guanylyl cyclase in rat cerebral cortex during postnatal development.
61-7000 was used in immunohistochemistry to test if the NO/cGMP pathway contributes to neural development in vivo.
|Ding JD,Burette A,Weinberg RJ||The Journal of comparative neurology (485:255)||2005|
Distribution of soluble guanylyl cyclase in the rat brain.
61-7000 was used in immunohistochemistry to examine the distribution of sGC subunits in the brain of adult rats.
|Ding JD,Burette A,Nedvetsky PI,Schmidt HH,Weinberg RJ||The Journal of comparative neurology (472:437)||2004|
|Not Applicable||Not Cited||
The elevation of intraocular pressure is associated with apoptosis and increased immunoreactivity for nitric oxide synthase in rat retina whereas the effectiveness of retina derived relaxing factor is unaffected.
61-7000 was used in immunohistochemistry - paraffin section to investigate the association with apoptosis and increased immunoreactivity for nitric oxide synthase in rat retina causing an elevation of intraocular pressure and how the retina derived relax
|Tak¿r S,Gürel-Gürevin E,Toprak A,Demirci-Tansel C,Uyde¿-Do¿an BS||Experimental eye research (145:401)||2016|
Homeostatic sleep pressure is the primary factor for activation of cortical nNOS/NK1 neurons.
61-7000 was used in immunohistochemistry - paraffin section to study the effects of sleep and wake duration on cortical nNOS/NK1 neuron activation.
|Dittrich L,Morairty SR,Warrier DR,Kilduff TS||Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (40:632)||2015|
Megaesophagus in a line of transgenic rats: a model of achalasia.
61-7000 was used in immunohistochemistry - paraffin section to characterize the use of Pvrl3-Cre transgenic rats to use as an animal model of megaesophagus and achalasia.
|Pang J,Borjeson TM,Muthupalani S,Ducore RM,Carr CA,Feng Y,Sullivan MP,Cristofaro V,Luo J,Lindstrom JM,Fox JG||Veterinary pathology (51:1187)||2014|
|Not Applicable||1 µg/ml||
Role of renal cortical cyclooxygenase-2 expression in hyperfiltration in rats with high-protein intake.
61-7000 was used in immunohistochemistry - paraffin section to propose that the nNOS and COX-2 systems regulate afferent arteriolar tone and subsequent hyperfiltration during high-protein intake
|Yao B,Xu J,Qi Z,Harris RC,Zhang MZ||American journal of physiology. Renal physiology (291:F368)||2006|
Effect of thermal energy produced by drilling on the facial nerve: histopathologic evaluation in guinea pigs.
61-7000 was used in immunohistochemistry - paraffin section to assess effect of thermal energy due to drilling around the facial nerve canal on the facial nerve using guinea pigs
|Aslan A,Vatansever HS,Aslan GG,Eskiizmir G,Giray G||The Journal of laryngology and otology (119:600)||2005|
Upregulation of nitric oxide synthase in mice with severe hypoxia-induced pulmonary hypertension.
61-7000 was used in immunohistochemistry - paraffin section to determine how hypoxia alters NOS expression in wild-type mice.
|Fagan KA,Morrissey B,Fouty BW,Sato K,Harral JW,Morris KG,Hoedt-Miller M,Vidmar S,McMurtry IF,Rodman DM||Respiratory research (2:306)||2001|
Prolonged high fat diet ingestion, obesity, and type 2 diabetes symptoms correlate with phenotypic plasticity in myenteric neurons and nerve damage in the mouse duodenum.
61-7000 was used in immunohistochemistry (frozen) to characterize the long term effects of diabetic enteric neuropathy using mouse models.
|Stenkamp-Strahm CM,Nyavor YE,Kappmeyer AJ,Horton S,Gericke M,Balemba OB||Cell and tissue research (361:411)||2015|
Ferulic acid inhibits nitric oxide-induced apoptosis by enhancing GABA(B1) receptor expression in transient focal cerebral ischemia in rats.
61-7000 was used in immunohistochemistry to assess the anti-apoptotic effect of ferulic acid during reperfusion after cerebral ischemia
|Cheng CY,Su SY,Tang NY,Ho TY,Lo WY,Hsieh CL||Acta pharmacologica Sinica (31:889)||2010|
Hyperpolarization-activated ion channels as targets for nitric oxide signalling in deep cerebellar nuclei.
61-7000 was used in immunohistochemistry to investigate the effects of cGMP signaling on hyperpolarization-activated cyclic nucleotide-modulated cation channels
|Wilson GW,Garthwaite J||The European journal of neuroscience (31:1935)||2010|
|Mouse||1:1000||Tetrahydrobiopterin deficiency induces gastroparesis in newborn mice.||Welsh C,Enomoto M,Pan J,Shifrin Y,Belik J||American journal of physiology. Gastrointestinal and liver physiology (305:G47)||2013|
Fatigue and muscle atrophy in a mouse model of myasthenia gravis is paralleled by loss of sarcolemmal nNOS.
61-7000 was used in western blot to assess the role of nNOS in chronic muscle fatigue and muscle atrophy.
|Meinen S,Lin S,Rüegg MA,Punga AR||PloS one (7:null)||2012|
Nitric oxide production by mouse renal tubules can be increased by a sodium-dependent mechanism.
61-7000 was used in immunohistochemistry and western blot to examine the sodium-nitric oxide linkage in cortical tubules
|Kempson S,Thompson N,Pezzuto L,Glenn Bohlen H||Nitric oxide : biology and chemistry (17:33)||2007|
|Not Applicable||Not Cited||
Conditional neuronal nitric oxide synthase overexpression impairs myocardial contractility.
61-7000 was used in western blot to propose that nNOS suppresses L-type Ca(2+)-channel function to regulate myocardial contractility
|Burkard N,Rokita AG,Kaufmann SG,Hallhuber M,Wu R,Hu K,Hofmann U,Bonz A,Frantz S,Cartwright EJ,Neyses L,Maier LS,Maier SK,Renné T,Schuh K,Ritter O||Circulation research (100:e32)||2007|
|Mouse||Not Cited||Divergent contractile and structural responses of the murine PKC-epsilon null pulmonary circulation to chronic hypoxia.||Littler CM,Wehling CA,Wick MJ,Fagan KA,Cool CD,Messing RO,Dempsey EC||American journal of physiology. Lung cellular and molecular physiology (289:L1083)||2005|
Analysis of the cytosolic proteome in a cell culture model of familial amyotrophic lateral sclerosis reveals alterations to the proteasome, antioxidant defenses, and nitric oxide synthetic pathways.
61-7000 was used in immunohistochemistry and western blot to study the revelation of alterations to the antioxidant defenses, proteasome, and nitric oxide synthetic pathways determined by analysis of the cystosolic proteome in a cell culture model of fam
|Allen S,Heath PR,Kirby J,Wharton SB,Cookson MR,Menzies FM,Banks RE,Shaw PJ||The Journal of biological chemistry (278:6371)||2003|
|Mouse||Not Cited||Neurturin regulates postnatal differentiation of parasympathetic pelvic ganglion neurons, initial axonal projections, and maintenance of terminal fields in male urogenital organs.||Yan H,Keast JR||The Journal of comparative neurology (507:1169)||2008|
Expression of receptors for glial cell line-derived neurotrophic factor family ligands in sacral spinal cord reveals separate targets of pelvic afferent fibers.
61-7000 was used in immunohistochemistry to localize the receptors for glial cell line-derived neurotrophic factor family ligands in sacral spinal cord and their roles in neuronal plasticity
|Forrest SL,Keast JR||The Journal of comparative neurology (506:989)||2008|
|Rat||Not Cited||Distribution of soluble guanylyl cyclase in rat retina.||Ding JD,Weinberg RJ||The Journal of comparative neurology (502:734)||2007|
|Mouse||Not Cited||High concentration of glucose inhibits glomerular endothelial eNOS through a PKC mechanism.||Chu S,Bohlen HG||American journal of physiology. Renal physiology (287:F384)||2004|
|Mouse||Not Cited||Stimulation of calcineurin signaling attenuates the dystrophic pathology in mdx mice.||Chakkalakal JV,Harrison MA,Carbonetto S,Chin E,Michel RN,Jasmin BJ||Human molecular genetics (13:379)||2004|
|Mouse||1:200||Layer positioning of late-born cortical interneurons is dependent on Reelin but not p35 signaling.||Hammond V,So E,Gunnersen J,Valcanis H,Kalloniatis M,Tan SS||The Journal of neuroscience : the official journal of the Society for Neuroscience (26:1646)||2006|
|Human||Not Cited||PSD-95 assembles a ternary complex with the N-methyl-D-aspartic acid receptor and a bivalent neuronal NO synthase PDZ domain.||Christopherson KS,Hillier BJ,Lim WA,Bredt DS||The Journal of biological chemistry (274:27467)||1999|