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Invitrogen
nNOS Polyclonal Antibody
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FIGURE: 1 / 12
nNOS Antibody (PA1-033) in ICC/IF
Immunofluorescent analysis of nNOS (green) showing staining in the cytoplasm and membrane of U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an nNOS polyclonal antibody (Product # PA1-033) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLig... View More
Product Details
PA1-033
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues C N(1411) R L R S E S I A F I E E S K(1425) of human nNOS.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-033 detects nNOS from human, rat and mouse samples.
PA1-033 has been successfully used in Western blot, ICC/IF and immunohistochemisty (paraffin) procedures. By Western blot, this antibody detects an ~160 kDa protein. Detects a few non-specific bands in Western blot analysis of rat and mouse brain lysates. This antibody is not recommended for MCF-7, PC-12, SK-N-C or U87-MG cells in Western blot applications.
The PA1-033 immunogen is a synthetic peptide to residues C N(1411) R L R S E S I A F I E E S K(1425) of human nNOS. The peptide sequence is 100% conserved in rat, mouse, frog, and rabbit nNos proteins. This peptide (Cat. # PEP-190) is available for use in neutralization and control experiments.
Target Information
NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: bNOS; bNOS1; Brain NOS; Constitutive NOS; N-NOS; NC-NOS; Neuronal NOS; nitric oxidase synthase; Nitric oxide synthase 1; nitric oxide synthase 1 (neuronal); Nitric oxide synthase, brain; nNOS; NOS Type 1; NOS type I; Peptidyl-cysteine S-nitrosylase NOS1; Phospho-bNOS; Phospho-Brain NOS
Gene Aliases: 2310005C01Rik; Bnos; IHPS1; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; NOS-I; NOS1
UniProt ID: (Human) P29475, (Rat) P29476, (Mouse) Q9Z0J4
Entrez Gene ID: (Human) 4842, (Rat) 24598, (Mouse) 18125
nitric-oxide synthase activity
iron ion binding
protein binding
calmodulin binding
FMN binding
sodium channel regulator activity
enzyme binding
heme binding
tetrahydrobiopterin binding
arginine binding
protein homodimerization activity
ion channel binding
cadmium ion binding
flavin adenine dinucleotide binding
NADP binding
scaffold protein binding
NADPH-hemoprotein reductase activity
amino acid binding
ATPase binding
oxidoreductase
response to hypoxia
regulation of sodium ion transport
arginine catabolic process
nitric oxide biosynthetic process
striated muscle contraction
nitric oxide mediated signal transduction
myoblast fusion
female pregnancy
aging
negative regulation of cell proliferation
response to heat
response to lead ion
negative regulation of calcium ion transport into cytosol
response to activity
peptidyl-cysteine S-nitrosylation
positive regulation of guanylate cyclase activity
response to lipopolysaccharide
response to vitamin E
multicellular organismal response to stress
positive regulation of histone acetylation
response to nicotine
neurotransmitter biosynthetic process
exogenous drug catabolic process
negative regulation of apoptotic process
negative regulation of potassium ion transport
response to peptide hormone
response to estrogen
cell redox homeostasis
response to ethanol
negative regulation of blood pressure
negative regulation of heart contraction
positive regulation of transcription, DNA-templated
negative regulation of vasoconstriction
positive regulation of vasodilation
positive regulation of transcription from RNA polymerase II promoter
negative regulation of insulin secretion
behavioral response to cocaine
regulation of neurogenesis
negative regulation of hydrolase activity
negative regulation of cytosolic calcium ion concentration
negative regulation of serotonin uptake
negative regulation of calcium ion transport
regulation of sensory perception of pain
oxidation-reduction process
regulation of cardiac muscle contraction
regulation of ryanodine-sensitive calcium-release channel activity
cellular response to mechanical stimulus
cellular response to growth factor stimulus
response to nitric oxide
positive regulation of the force of heart contraction
positive regulation of long-term synaptic potentiation
negative regulation of adrenergic receptor signaling pathway involved in heart process
positive regulation of adrenergic receptor signaling pathway involved in heart process
positive regulation of neuron death
positive regulation of sodium ion transmembrane transport
regulation of calcium ion transmembrane transport via high voltage-gated calcium channel
regulation of cardiac conduction
muscle contraction
regulation of heart contraction
response to organonitrogen compound
response to organic cyclic compound
response to nutrient levels
negative regulation of peptidyl-serine phosphorylation
cellular response to epinephrine stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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