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|Tested species reactivity||Rat|
|Published species reactivity||Rat , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues I(237) Q V D R D L D G K S H K A(250) of rat nNOS.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-038 detects recombinant rat brain nitric oxide synthase (nNOS) overexpressed in E. coli. This antibody does not to detect iNOS or eNOS.
PA1-038 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~155 kDa protein representing recombinant rat nNOS protein overexpressed in E. coli.
The PA1-038 immunogen is a synthetic peptide corresponding to residues I(237) Q V D R D L D G K S H K A(250) of rat nNOS.
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.
bNOS and eNOS share approximately 50% sequence homology and their enzymatic activity depends on binding to the calcium/calmodulin complex. Increases in intracellular calcium lead to the production of low levels of NO over a short time. bNOS is found in neurons, peripheral nerve cells, macula densa, and pancreatic islet cells. Alternate splicing specifically regulates bNOS in striated muscle. The translated protein, bNOS mu, is 34 amino acid residues larger than bNOS from brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Blastocyst injection of wild type embryonic stem cells induces global corrections in mdx mice.
PA1-038 was used in western blot to study the rescue of mdx mice by wild type embryonic stem cells
|Stillwell E,Vitale J,Zhao Q,Beck A,Schneider J,Khadim F,Elson G,Altaf A,Yehia G,Dong JH,Liu J,Mark W,Bhaumik M,Grange R,Fraidenraich D||PloS one (4:null)||2009|
Effects of sex differences on constitutive nitric oxide synthase expression and activity in response to pressure overload in rats.
PA1-038 was used in western blot to study gender differences in constitutive NOS expression and activity in pressure overloaded rats
|Loyer X,Oliviero P,Damy T,Robidel E,Marotte F,Heymes C,Samuel JL||American journal of physiology. Heart and circulatory physiology (293:H2650)||2007|
Altered contractile response due to increased beta3-adrenoceptor stimulation in diabetic cardiomyopathy: the role of nitric oxide synthase 1-derived nitric oxide.
PA1-038 was used in western blot to study the role of NOS-1-derived NO in the altered contractile response due to increased beta3-adrenoceptor stimulation in diabetic cardiomyopathy
|Amour J,Loyer X,Le Guen M,Mabrouk N,David JS,Camors E,Carusio N,Vivien B,Andriantsitohaina R,Heymes C,Riou B||Anesthesiology (107:452)||2007|
Levels of protein kinase C and nitric oxide synthase activity in rats exposed to sub chronic low level lead.
PA1-038 was used in western blot to study levels of protein kinase C and NOS activity in brain regions of rats exposed to sub chronic low level lead
|Ramesh GT,Jadhav AL||Molecular and cellular biochemistry (223:27)||2001|
Neuronal nitric-oxide synthase-mu, an alternatively spliced isoform expressed in differentiated skeletal muscle.
PA1-038 was used in western blot to investigate the distribution of nitric-oxide synthase mu in differentiated skeletal muscle
|Silvagno F,Xia H,Bredt DS||The Journal of biological chemistry (271:11204)||1996|
Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase.
PA1-038 was used in western blot to investigate the characteristics of nitric oxide synthase in vivo
|Bredt DS,Hwang PM,Glatt CE,Lowenstein C,Reed RR,Snyder SH||Nature (351:714)||1991|
Reactive nitrogen and oxygen species in airway inflammation.
PA1-038 was used in immunohistochemistry to review the role of reactive nitrogen and oxygen species in airway inflammation
|Folkerts G,Kloek J,Muijsers RB,Nijkamp FP||European journal of pharmacology (429:251)||2001|
bNOS1; Brain NOS; constitutive NOS; N-NOS; NC-NOS; Neuronal Nitric Oxide Synthase; neuronal NOS; nitric oxidase synthase; nitric oxide synthase, brain; nNOS; NOS Type 1; NOS type I; peptidyl-cysteine S-nitrosylase NOS1