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Invitrogen
nNOS Polyclonal Antibody
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FIGURE: 1 / 1
nNOS Antibody (PA1-038) in WB
PloS one 2009 -
Figure 10 WT/mdx muscle displays nNOS and dystrobrevin-alpha. Protein extracts from diaphragms of WT (lane 1), 10-30% WT/mdx (lane 2) and mdx (lane 3) mice were subjected to a western blot for dystrophin, nNOS, dystrobrevin-alpha, syntrophin and tubulin. *: dystrobrevin-alpha isoforms enriched in mdx and WT/mdx but not in WT muscle. Experiments were performed with pectoralis, diaphragm and quadriceps muscle... View More
Product Details
PA1-038
Applications
Tested Dilution
Publications
Product Specifications
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues I(237) Q V D R D L D G K S H K A(250) of rat nNOS.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
Product Specific Information
PA1-038 detects recombinant rat brain nitric oxide synthase (nNOS) overexpressed in E. coli. This antibody does not to detect iNOS or eNOS.
PA1-038 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~155 kDa protein representing recombinant rat nNOS protein overexpressed in E. coli.
The PA1-038 immunogen is a synthetic peptide corresponding to residues I(237) Q V D R D L D G K S H K A(250) of rat nNOS.
Target Information
NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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