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Bethyl Laboratories
p115-RhoGEF Polyclonal Antibody
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FIGURE: 1 / 1
p115-RhoGEF Antibody (A302-702A) in IP
Detection of human p115-RhoGEF by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-p115-RhoGEF antibody (Product # A302-702A) used for IP at 3 µg/mg lysate. p115-RhoGEF was also immunoprecipitated by rabbit anti-p115-RhoGEF antibody A302-701A, which recognizes an upstream epitope. For blotting immunoprecipitated p115-RhoGEF, A302-701A was used at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
Product Details
A302-702A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 775 and 825 of human 115 kDa Rho guanine nucleotide exchange factor.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
The Ras superfamily of GTPases, which can be subdivided into the Ras, Rho/Rac, Sar, Rab, ARF and Ran subfamilies, controls multiple aspects of cell function, including cytoskeletal rearrangement, nuclear signaling and cell growth. The Ras superfamily of GTPases function as regulated switches that toggle between a biologically active GTP-bound and an inactive GDP-bound form. This activation is catalyzed by guanine nucleotide exchange factors (GEFs). The Dbl-related proteins are a large family of structurally related molecules that have a common ability to catalyze GEF activity for specific members of the Ras family. Dbl-related proteins include FGD1, Lsc, RhoGEF p115, Lfc, Lbc and Brx. Lsc, Lbc and Lfc share sequence homology and show exchange activity toward Rho family GTPases. RhoGEF p115 catalyzes GEF activity for Rho but not Rac, Cdc42 or Ras GTPases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 115 kDa guanine nucleotide exchange factor; 115-kD protein; Lsc homolog; p115-RhoGEF; p115RhoGEF; Rho guanine nucleotide exchange factor (GEF) 1; Rho guanine nucleotide exchange factor 1; Sub1.5
Gene Aliases: ARHGEF1; GEF1; IMD62; LBCL2; LSC; P115-RHOGEF; SUB1.5
UniProt ID: (Human) Q92888
Entrez Gene ID: (Human) 9138
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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