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p130 Rb2 Polyclonal Antibody
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FIGURE: 1 / 2
p130 Rb2 Antibody (100-401-173) in IHC (P)
Immunohistochemical staining of mouse tissue using anti-pRb2/p130 antiserum. The staining shows the location of pRb2/p130 in developing mouse tissue. Other detection systems should yield similar results. Sections were cut at 5-7 µm, mounted on glass and dried overnight at 37°C. All sections were deparaffinized in xylene, rehydrated through a graded alcohol series and washed in phosphate-buffered saline (PBS... View More
Product Details
100-401-173
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Rb2 (p130) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugate
Form
Liquid
Concentration
85 mg/mL
Storage buffer
0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Antiserum will specifically react with a 130 kDa Rb2 protein from human, rat and mouse tissue. No reaction was observed against other related tumor supressor proteins. Cross reactivity with Rb2 (p130) from other species may also occur.
Target Information
RBL2 (also known as p130) or Retinoblastoma-like protein 2 is a key regulator of entry into cell division. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and (in particular) that of constitutive heterochromatin by stabilizing histone methylation. RBL2 recruits and targets histone methyltransferases KMT5B and KMT5C leading to epigenetic transcriptional repression. It controls histone H4 'Lys20' trimethylation. It probably also acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. RBL2 serves as a potent inhibitor of E2F-mediated trans-activation and associates preferentially with E2F5.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 130 kDa retinoblastoma-associated protein; E1A-associated cyclin-binding protein; FLJ26459; P130; PPAR-alpha-interacting complex protein 128; PRB2; PRIC128; rb2 p130; RBR-2; retinoblastoma-like 2; Retinoblastoma-like protein 2; Retinoblastoma-related protein 2; unnamed protein product
Gene Aliases: BRUWAG; P130; PRB2; RB2; RBL2; RBR-2
UniProt ID: (Human) Q08999, (Mouse) Q64700, (Rat) O55081
Entrez Gene ID: (Human) 5934, (Mouse) 19651, (Rat) 81758
chromatin organization
regulation of transcription from RNA polymerase II promoter
negative regulation of gene expression
cell differentiation
regulation of lipid kinase activity
regulation of cell cycle
negative regulation of G1/S transition of mitotic cell cycle
transcription, DNA-templated
regulation of transcription, DNA-templated
cell cycle
chromatin modification
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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