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Invitrogen

p14ARF Polyclonal Antibody

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p14ARF Polyclonal Antibody

Product Details

PA1-46021

Applications
Tested Dilution
Publications

Western Blot (WB)

2 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide made to a portion of human p14ARF (between residues 100-173).
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.5 mg/mL

Amount

250 µg

Purification

Antigen affinity chromatography

Storage buffer

TBS

Contains

0.05% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_923425

Product Specific Information

By Western blot, PA1-46021 is specific for the ~16 kDa CDKN2A protein (CDKN2A tends to run slightly higher than the theoretical MW of 14 kDa).

Additional non-specific bands may be seen at ~25 and 47 kDa.

Suggested positive control: BT549 cells or HeLa whole cell extract, antigen standard for CDKN2A (transient overexpression lysate).

Target Information

The INK4a locus on chromosome 9p21 is frequently affected in human tumors. It encodes two structurally distinct tumor suppressor proteins, p16INK4a and the alternative reading frame protein, ARF (p14ARF in human and P19ARF in mouse). These two proteins interact with the upstream proteins: retinoblastoma protein and p53 tumor suppressors, respectively. Each of these proteins has a role in the senescence of primary cells, activates pathways for cell cycle control and tumor suppression. The p14ARF (or P19ARF in mouse) proteins can induce both G1 and G2 phase arrest in a manner that depends on functional p53. The mouse and human ARF proteins have different functions in tumor suppression; this distinction may contribute to the different levels of tumor proneness of these species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: a frameshift between exon 1 (0.18) and exon 2 changed the ORF of p16INK4 gene.; Alternative reading frame; ARF; CDK4 inhibitor p16-INK4; CDK4I; CDKN2A/ARF Intron 2 lncRNA; cell cycle negative regulator beta; Cyclin-dependent kinase 4 inhibitor A; Cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4); hypothetical 18.1 kDa protein from longest open reading frame; inhibitor of cdk4 A; inhibits CDK4; MTS-1; Multiple tumor suppressor 1; multiple tumour suppressor 1; p14 p16; p14ARF; p16; p16 gamma; p16-INK4; p16-INK4a; P19 TP16; Tumor suppressor ARF

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Gene Aliases: ARF; CAI2; CDK4I; CDKN2; CDKN2A; CMM2; INK4; INK4A; MLM; MTS-1; MTS1; P14; P14ARF; P16; P16-INK4A; P16INK4; P16INK4A; P19; P19ARF; TP16

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UniProt ID: (Human) P42771

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Entrez Gene ID: (Human) 1029

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Function(s)
p53 binding DNA binding RNA binding cyclin-dependent protein serine/threonine kinase inhibitor activity protein binding SUMO transferase activity protein kinase binding NF-kappaB binding ligase inhibitor activity ubiquitin-protein transferase inhibitor activity RNA polymerase II sequence-specific DNA binding transcription factor binding MDM2/MDM4 family protein binding disordered domain specific binding ubiquitin ligase inhibitor activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter protein polyubiquitination mitophagy negative regulation of cell-matrix adhesion rRNA processing apoptotic process Ras protein signal transduction negative regulation of cell proliferation epidermis development rRNA transcription regulation of gene expression positive regulation of gene expression protein sumoylation keratinocyte differentiation negative regulation of cell growth nuclear body organization negative regulation of B cell proliferation regulation of protein stability protein destabilization negative regulation of immature T cell proliferation in thymus positive regulation of protein sumoylation mammary gland epithelial cell proliferation negative regulation of mammary gland epithelial cell proliferation positive regulation of smooth muscle cell apoptotic process protein localization to nucleus somatic stem cell population maintenance glucose homeostasis positive regulation of apoptotic process positive regulation of DNA damage response, signal transduction by p53 class mediator keratinocyte proliferation negative regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of nucleocytoplasmic transport regulation of protein export from nucleus somatic stem cell division protein stabilization regulation of cell cycle mitochondrial depolarization apoptotic process involved in mammary gland involution positive regulation of apoptotic process involved in mammary gland involution cellular response to hydrogen peroxide protein K63-linked ubiquitination cellular senescence replicative senescence oncogene-induced cell senescence apoptotic signaling pathway positive regulation of protein localization to nucleus positive regulation of signal transduction by p53 class mediator protein localization to nucleolus negative regulation of proteolysis involved in cellular protein catabolic process regulation of protein targeting to mitochondrion negative regulation of ubiquitin protein ligase activity amyloid fibril formation regulation of G1/S transition of mitotic cell cycle negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of macrophage apoptotic process negative regulation of protein neddylation
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