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Invitrogen
p14ARF Polyclonal Antibody
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Product Details
PA1-46021
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide made to a portion of human p14ARF (between residues 100-173).
Conjugate
Form
Liquid
Concentration
2.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS
Contains
0.05% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
By Western blot, PA1-46021 is specific for the ~16 kDa CDKN2A protein (CDKN2A tends to run slightly higher than the theoretical MW of 14 kDa).
Additional non-specific bands may be seen at ~25 and 47 kDa.
Suggested positive control: BT549 cells or HeLa whole cell extract, antigen standard for CDKN2A (transient overexpression lysate).
Target Information
The INK4a locus on chromosome 9p21 is frequently affected in human tumors. It encodes two structurally distinct tumor suppressor proteins, p16INK4a and the alternative reading frame protein, ARF (p14ARF in human and P19ARF in mouse). These two proteins interact with the upstream proteins: retinoblastoma protein and p53 tumor suppressors, respectively. Each of these proteins has a role in the senescence of primary cells, activates pathways for cell cycle control and tumor suppression. The p14ARF (or P19ARF in mouse) proteins can induce both G1 and G2 phase arrest in a manner that depends on functional p53. The mouse and human ARF proteins have different functions in tumor suppression; this distinction may contribute to the different levels of tumor proneness of these species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alternative reading frame; ARF; CDK4 inhibitor p16-INK4; CDK4I; cell cycle negative regulator beta; Cyclin-dependent kinase 4 inhibitor A; Cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4); inhibits CDK4; MTS-1; Multiple tumor suppressor 1; p14 p16; p14ARF; p16-INK4; p16-INK4a; P19 TP16; Tumor suppressor ARF
Gene Aliases: ARF; CDK4I; CDKN2; CDKN2A; CMM2; INK4; INK4A; MLM; MTS-1; MTS1; P14; P14ARF; P16; P16-INK4A; P16INK4; P16INK4A; P19; P19ARF; TP16
UniProt ID: (Human) P42771
Entrez Gene ID: (Human) 1029
G1/S transition of mitotic cell cycle
negative regulation of cell-matrix adhesion
cell cycle arrest
Ras protein signal transduction
negative regulation of cell proliferation
negative regulation of cell growth
negative regulation of NF-kappaB transcription factor activity
positive regulation of smooth muscle cell apoptotic process
senescence-associated heterochromatin focus assembly
negative regulation of phosphorylation
negative regulation of cyclin-dependent protein serine/threonine kinase activity
negative regulation of transcription, DNA-templated
cellular senescence
replicative senescence
positive regulation of macrophage apoptotic process
positive regulation of cellular senescence
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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