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p14ARF Monoclonal Antibody (4C6/4)

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p14ARF Monoclonal Antibody (4C6/4)

Product Details

1029-MSM1-P0

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1-2 µg/mL
-

Immunocytochemistry (ICC/IF)

1-2 µg/mL
-

Flow Cytometry (Flow)

1-2 µg/10^6 cells
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

4C6/4

Immunogen

Recombinant full-length human p14 ARF protein
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Amount

20 µg

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

The INK4a locus on chromosome 9p21 is frequently affected in human tumors. It encodes two structurally distinct tumor suppressor proteins, p16INK4a and the alternative reading frame protein, ARF (p14ARF in human and P19ARF in mouse). These two proteins interact with the upstream proteins: retinoblastoma protein and p53 tumor suppressors, respectively. Each of these proteins has a role in the senescence of primary cells, activates pathways for cell cycle control and tumor suppression. The p14ARF (or P19ARF in mouse) proteins can induce both G1 and G2 phase arrest in a manner that depends on functional p53. The mouse and human ARF proteins have different functions in tumor suppression; this distinction may contribute to the different levels of tumor proneness of these species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: a frameshift between exon 1 (0.18) and exon 2 changed the ORF of p16INK4 gene.; Alternative reading frame; ARF; CDK4 inhibitor p16-INK4; CDK4I; cdkn2a {ECO:0000312|EMBL:AAL76336.1, ECO:0000312|RGD:2323}; cdkn2a {ECO:0000312|EMBL:AAL76338.1, ECO:0000312|RGD:2323}; CDKN2A/ARF Intron 2 lncRNA; cell cycle inhibitor; cell cycle negative regulator beta; cell cycle regulator; Cyclin dependent kinase inhibitor 2A (p16, inhibits CDK4); Cyclin-dependent kinase 4 inhibitor A; Cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2a p16Ink4a; cyclin-dependent kinase inhibitor 2a p19Arf; cyclin-dependent kinase inhibitor 2A, isoform 1; cyclin-dependent kinase inhibitor 2A, isoform 2; hypothetical 18.1 kDa protein from longest open reading frame; inhibitor of cdk4 A; inhibits CDK4; MTS-1; Multiple tumor suppressor 1; multiple tumour suppressor 1; p14 p16; p14ARF; p16; p16 gamma; p16-INK4; p16-INK4a; P19 TP16; Tumor suppressor ARF

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Gene Aliases: ARF; CAI2; CDK4I; CDKN2; CDKN2A; CMM2; INK4; INK4A; MLM; MTS-1; MTS1; P14; P14ARF; P16; P16-INK4A; p16Cdkn2a; P16INK4; P16INK4A; P19; P19ARF; TP16

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UniProt ID: (Human) P42771, (Rat) Q9R0Z3

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Entrez Gene ID: (Human) 1029, (Rat) 25163

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Function(s)
p53 binding DNA binding RNA binding cyclin-dependent protein serine/threonine kinase inhibitor activity protein binding SUMO transferase activity protein kinase binding NF-kappaB binding ligase inhibitor activity ubiquitin-protein transferase inhibitor activity RNA polymerase II sequence-specific DNA binding transcription factor binding MDM2/MDM4 family protein binding disordered domain specific binding ubiquitin ligase inhibitor activity transcription factor activity, sequence-specific DNA binding transcription factor binding poly(A) RNA binding
Process(es)
regulation of cyclin-dependent protein serine/threonine kinase activity G1/S transition of mitotic cell cycle protein polyubiquitination negative regulation of cell-matrix adhesion transcription, DNA-templated regulation of transcription, DNA-templated rRNA processing negative regulation of protein kinase activity apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process cell cycle arrest Ras protein signal transduction aging cell aging negative regulation of cell proliferation epidermis development apoptotic mitochondrial changes rRNA transcription response to organic substance response to organonitrogen compound regulation of G2/M transition of mitotic cell cycle regulation of gene expression response to organic cyclic compound cerebellum development negative regulation of cell growth negative regulation of B cell proliferation regulation of protein stability protein destabilization negative regulation of NF-kappaB transcription factor activity negative regulation of protein binding negative regulation of immature T cell proliferation in thymus positive regulation of protein sumoylation negative regulation of mammary gland epithelial cell proliferation positive regulation of smooth muscle cell apoptotic process somatic stem cell population maintenance senescence-associated heterochromatin focus assembly negative regulation of phosphorylation response to drug glucose homeostasis positive regulation of apoptotic process positive regulation of DNA damage response, signal transduction by p53 class mediator negative regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of nucleocytoplasmic transport regulation of protein export from nucleus somatic stem cell division protein stabilization positive regulation of sequence-specific DNA binding transcription factor activity negative regulation of ubiquitin-protein transferase activity positive regulation of apoptotic process involved in mammary gland involution cellular response to hydrogen peroxide protein K63-linked ubiquitination positive regulation of cell cycle arrest cellular senescence replicative senescence positive regulation of protein localization to nucleus positive regulation of signal transduction by p53 class mediator regulation of apoptotic DNA fragmentation negative regulation of proteolysis involved in cellular protein catabolic process negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of macrophage apoptotic process positive regulation of cellular senescence negative regulation of transcription from RNA polymerase II promoter mitophagy positive regulation of gene expression protein sumoylation keratinocyte differentiation nuclear body organization mammary gland epithelial cell proliferation protein localization to nucleus keratinocyte proliferation regulation of cell cycle mitochondrial depolarization apoptotic process involved in mammary gland involution oncogene-induced cell senescence apoptotic signaling pathway protein localization to nucleolus regulation of protein targeting to mitochondrion negative regulation of ubiquitin protein ligase activity amyloid fibril formation regulation of G1/S transition of mitotic cell cycle negative regulation of protein neddylation
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