Search
Invitrogen
p21 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 4
p21 Antibody (PA1-30399) in WB
Oncology reports 2021 -
Figure 6. (A) Immunocytochemical staining for CK7, p63 and DeltaNp63 in primary cultured cancer cells derived from human head and neck squamous cell carcinoma tissues. Scale bar, 10 um. (B) Phase-contrast images. Scale bar, 50 um. (C) Western blotting for p63, JAM-A and claudin-1, and (D) immunocytochemical staining for JAM-A and claudin-1 in primary cultured cancer cells treated with 10 uM HDAC inhibitors.... View More
Product Details
PA1-30399
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide mapping to the carboxy terminus of human p21.
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
200 µg
Purification
Affinity chromatography
Storage buffer
PBS with 0.2% gelatin
Contains
0.1% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: C32.
Target Information
Protein p21 is a tumor suppressor protein which is an inhibitor of Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53. P21 is important in the response of cells to genotoxic stress and a major transcriptional target of p53 protein. The occurrence of p21 in the nucleus executes binding and inhibition activity of cyclin dependent kinases Cdk1 and Cdk2, and blocks the transition from G1 phase into S phase or from G2 phase into mitosis after DNA damage, which enables the repair of damaged DNA. In the cytoplasm, p21 protein has an anti-apoptotic effect. P21 is able to bind to and inhibit caspase 3, as well as the apoptotic kinases ASK1 and JNK. P21 exhibits a dual function in carcinogenesis, and acts as a tumor suppressor, prevents apoptosis, and acts as an oncogene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases:
Activating Fragment 1; alternate gene name=WAF1; CDK-interacting protein 1; CDK-interaction protein 1; CDKN1A; CDNK1A; Cip1); cyclin kinase inhibitor; Cyclin-dependent kinase inhibitor 1; Cyclin-dependent kinase inhibitor 1A (p21; cyclin-dependent kinase inhibitor 1A (P21); cyclin-dependent kinase inhibitor 1A (p21, Cip1); cyclin-dependent kinase inhibitor 1A variant 1; cyclin-dependent kinase inhibitor 1A variant 2; DNA synthesis inhibitor; MDA-6; melanoma differentiation associated protein 6; Melanoma differentiation-associated protein; Melanoma differentiation-associated protein 6; OTTHUMP00000016298; p21; p21
Gene Aliases: CAP20; CDKI; CDKN1; CDKN1A; CIP1; MDA-6; MDA6; P21; p21CIP1; p21WAF; PIC1; SDI1; UV96; WAF1
UniProt ID: (Human) P38936, (Mouse) P39689
Entrez Gene ID: (Human) 1026, (Mouse) 12575, (Rat) 114851
protein kinase inhibitor activity
cyclin-dependent protein serine/threonine kinase inhibitor activity
protein binding
zinc ion binding
protein kinase binding
cyclin binding
ubiquitin protein ligase binding
macromolecular complex binding
metal ion binding
protein serine/threonine kinase binding
protein sequestering activity
molecular function activator activity
molecular function inhibitor activity
cyclin-dependent protein kinase activating kinase activity
protein complex binding
kinase inhibitor
G1/S transition of mitotic cell cycle
in utero embryonic development
protein import into nucleus
cellular response to DNA damage stimulus
mitotic G2 DNA damage checkpoint
Ras protein signal transduction
regulation of mitotic cell cycle
heart development
positive regulation of cell proliferation
negative regulation of cell proliferation
epidermis development
response to UV
regulation of G2/M transition of mitotic cell cycle
negative regulation of macromolecule biosynthetic process
negative regulation of gene expression
keratinocyte differentiation
negative regulation of cell growth
DNA damage response, signal transduction by p53 class mediator
positive regulation of B cell proliferation
mitotic G1 DNA damage checkpoint
cellular response to amino acid starvation
wound healing
tissue regeneration
signal transduction in response to DNA damage
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
positive regulation of programmed cell death
keratinocyte proliferation
positive regulation of DNA replication
positive regulation of protein kinase activity
negative regulation of growth
fibroblast proliferation
positive regulation of fibroblast proliferation
nuclear import
regulation of cell cycle
cellular response to cell-matrix adhesion
cellular response to ionizing radiation
cellular response to gamma radiation
cellular response to UV-B
signal transduction by p53 class mediator
cellular senescence
replicative senescence
stress-induced premature senescence
oncogene-induced cell senescence
intrinsic apoptotic signaling pathway
regulation of cell cycle G1/S phase transition
negative regulation of cyclin-dependent protein kinase activity
negative regulation of vascular smooth muscle cell proliferation
negative regulation of cardiac muscle tissue regeneration
regulation of G1/S transition of mitotic cell cycle
negative regulation of G1/S transition of mitotic cell cycle
regulation of DNA biosynthetic process
negative regulation of DNA biosynthetic process
positive regulation of reactive oxygen species metabolic process
regulation of cyclin-dependent protein serine/threonine kinase activity
G2/M transition of mitotic cell cycle
protein phosphorylation
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
cell cycle
cell cycle arrest
cellular response to extracellular stimulus
regulation of protein import into nucleus, translocation
negative regulation of phosphorylation
negative regulation of apoptotic process
negative regulation of cyclin-dependent protein serine/threonine kinase activity
mitotic cell cycle arrest
response to toxic substance
response to organic substance
response to X-ray
response to organonitrogen compound
response to organic cyclic compound
organ regeneration
cellular response to heat
response to drug
response to arsenic-containing substance
response to glucocorticoid
response to corticosterone
response to hyperoxia
intestinal epithelial cell maturation
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support