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Invitrogen

p21 Polyclonal Antibody

1 Reference
View all (80) p21 antibodies

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Product Details

PA5-34805

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
View 1 publication 1 publication
Product Specifications

Species Reactivity

Chicken, Human

Published species

Chicken

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human p21 Cip1. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2552157

Product Specific Information

Recommended positive controls: HeLa, HCT116 treated 30uM cisplatin 24hr.

Predicted reactivity: Cat (84%), Sheep (82%), Rhesus Monkey (98%), Bovine (83%).

IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Protein p21 is a tumor suppressor protein which is an inhibitor of Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53. P21 is important in the response of cells to genotoxic stress and a major transcriptional target of p53 protein. The occurrence of p21 in the nucleus executes binding and inhibition activity of cyclin dependent kinases Cdk1 and Cdk2, and blocks the transition from G1 phase into S phase or from G2 phase into mitosis after DNA damage, which enables the repair of damaged DNA. In the cytoplasm, p21 protein has an anti-apoptotic effect. P21 is able to bind to and inhibit caspase 3, as well as the apoptotic kinases ASK1 and JNK. P21 exhibits a dual function in carcinogenesis, and acts as a tumor suppressor, prevents apoptosis, and acts as an oncogene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Activating Fragment 1; alternate gene name=WAF1; cdk inhibitor CIP1 (p21); CDK-interacting protein 1; CDK-interaction protein 1; CDKN1A; CDNK1A; Cip1); cyclin kinase inhibitor; Cyclin-dependent kinase inhibitor 1; Cyclin-dependent kinase inhibitor 1A (p21; cyclin-dependent kinase inhibitor 1A (p21, Cip1); DNA synthesis inhibitor; MDA-6; melanoma differentiation associated protein 6; Melanoma differentiation-associated protein 6; OTTHUMP00000016298; p21; p21Cip1/Waf1; putative DNA synthesis inhibitor; SLC12A9; unnamed protein product; Wild type p53 activated fragment 1 (WAF1); wild type p53 activated fragment-1; wild-type p53-activated fragment 1

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Gene Aliases: CAP20; CDKN1; CDKN1A; CIP1; MDA-6; MDA6; P21; p21CIP1; PIC1; SDI1; WAF1

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UniProt ID: (Human) P38936

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Entrez Gene ID: (Chicken) 378914, (Human) 1026

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Function(s)
protein kinase inhibitor activity cyclin-dependent protein serine/threonine kinase inhibitor activity protein binding zinc ion binding protein kinase binding cyclin binding ubiquitin protein ligase binding macromolecular complex binding metal ion binding protein serine/threonine kinase binding protein sequestering activity molecular function activator activity molecular function inhibitor activity protein-binding activity modulator kinase inhibitor kinase modulator
Process(es)
G1/S transition of mitotic cell cycle in utero embryonic development protein import into nucleus cellular response to DNA damage stimulus mitotic G2 DNA damage checkpoint Ras protein signal transduction regulation of mitotic cell cycle heart development positive regulation of cell proliferation negative regulation of cell proliferation epidermis development response to UV regulation of G2/M transition of mitotic cell cycle negative regulation of macromolecule biosynthetic process negative regulation of gene expression keratinocyte differentiation negative regulation of cell growth DNA damage response, signal transduction by p53 class mediator positive regulation of B cell proliferation mitotic G1 DNA damage checkpoint cellular response to amino acid starvation wound healing tissue regeneration signal transduction in response to DNA damage intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of programmed cell death keratinocyte proliferation positive regulation of DNA replication positive regulation of protein kinase activity negative regulation of growth fibroblast proliferation positive regulation of fibroblast proliferation nuclear import regulation of cell cycle cellular response to cell-matrix adhesion cellular response to ionizing radiation cellular response to gamma radiation cellular response to UV-B signal transduction by p53 class mediator cellular senescence replicative senescence stress-induced premature senescence oncogene-induced cell senescence intrinsic apoptotic signaling pathway regulation of cell cycle G1/S phase transition negative regulation of cyclin-dependent protein kinase activity negative regulation of vascular smooth muscle cell proliferation negative regulation of cardiac muscle tissue regeneration regulation of G1/S transition of mitotic cell cycle negative regulation of G1/S transition of mitotic cell cycle regulation of DNA biosynthetic process negative regulation of DNA biosynthetic process positive regulation of reactive oxygen species metabolic process
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