Invitrogen

p21 Monoclonal Antibody (R.229.6)

p21 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 40

p21 Antibody (MA5-14949) in ICC/IF

Immunofluorescent analysis of p21 Waf1/Cip1 (green) in HeLa cells either left untreated (left panel) or treated with 5uM Camptothecin for 3 hours (right panel). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a p21 Waf1/Cip1 monoclonal antibody (Product # MA5-14949) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 ... View More

p21 Antibody in Immunoprecipitation (IP)

p21 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

p21 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

p21 Antibody in Western Blot, Immunohistochemistry, Flow Cytometry (WB, IHC, Flow)

Product Image Gallery

p21 Antibody — Modulation of expression of target protein by cell treatment demonstrates antibody specificity. Immunofluorescence analysis of p21 using p21 monoclonal antibody (Product # MA5-14949) shows induced expression of p21 in the nucleus of HeLa cells treated with Camptothecin. {TM}

Product Details

MA5-14949

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:800

View 13 publications 13 publications

Flow Cytometry (Flow)

1:100-1:400

View 2 publications 2 publications

Immunoprecipitation (IP)

1:50

Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

R.229.6

Immunogen

Synthetic peptide corresponding to residues near the carboxy-terminus of human p21

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

244 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10984461

Product Specific Information

Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other CDK inhibitors.

Target Information

Protein p21 is a tumor suppressor protein which is an inhibitor of Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53. P21 is important in the response of cells to genotoxic stress and a major transcriptional target of p53 protein. The occurrence of p21 in the nucleus executes binding and inhibition activity of cyclin dependent kinases Cdk1 and Cdk2, and blocks the transition from G1 phase into S phase or from G2 phase into mitosis after DNA damage, which enables the repair of damaged DNA. In the cytoplasm, p21 protein has an anti-apoptotic effect. P21 is able to bind to and inhibit caspase 3, as well as the apoptotic kinases ASK1 and JNK. P21 exhibits a dual function in carcinogenesis, and acts as a tumor suppressor, prevents apoptosis, and acts as an oncogene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Large-scale microRNA functional high-throughput screening identifies miR-515-3p and miR-519e-3p as inducers of human cardiomyocyte proliferation.

iScience

Renikunta HV,Lazarow K,Gong Y,Shukla PC,Nageswaran V,Giral H,Kratzer A,Opitz L,Engel FB,Haghikia A,Costantino S,Paneni F,von Kries JP,Streckfuss-Bömeke K,Landmesser U,Jakob P

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Western Blot

Fri May 19 00:00:00 UTC 2023

NFATc1 Regulation of Dexamethasone-Induced TGFB2 Expression Is Cell Cycle Dependent in Trabecular Meshwork Cells.

Cells

Filla MS,Faralli JA,Dunn CR,Khan H,Peters DM

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Immunohistochemistry (Paraffin)

Fri Feb 03 00:00:00 UTC 2023

NFATc1 Regulation of Dexamethasone-Induced TGFB2 Expression Is Cell Cycle Dependent in Trabecular Meshwork Cells.

Cells

Filla MS,Faralli JA,Dunn CR,Khan H,Peters DM

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Immunohistochemistry (Paraffin)

Fri Feb 03 00:00:00 UTC 2023

Single Fraction and Hypofractionated Radiation Cause Cochlear Damage, Hearing Loss, and Reduced Viability of Merlin-Deficient Schwann Cells.

Cancers

Dinh CT,Chen S,Nourbakhsh A,Padgett K,Johnson P,Goncalves S,Bracho O,Bas E,Bohorquez J,Monje PV,Fernandez-Valle C,Elsayyad N,Liu X,Welford SM,Telischi F

MA5-14949 was used in Immunocytochemistry to investigate the effects of single fraction and hypofractionated radiation on hearing thresholds in rats, cell death pathways in rat cochleae, and viability of human merlin-deficient Schwann cells (MD-SC).

Thu May 18 00:00:00 UTC 2023

KRT5⁺/p63⁺ Stem Cells Undergo Senescence in the Human Lung with Pathological Aging.

Aging and disease

Moreno-Valladares M,Moncho-Amor V,Silva TM,Garcés JP,Álvarez-Satta M,Matheu A

MA5-14949 was used in Immunohistochemistry-immunofluorescence to provide new evidence of the activity of p63+/KRT5+ stem cells on human lung regeneration and point out that regeneration machinery in human lung is activated under stress due to aging, but fails to repair in pathological cases, as stem cells would likely become senescent.

Thu Jun 01 00:00:00 UTC 2023

Cyanidin 3-O-arabinoside suppresses DHT-induced dermal papilla cell senescence by modulating p38-dependent ER-mitochondria contacts.

Journal of biomedical science

Jung YH,Chae CW,Choi GE,Shin HC,Lim JR,Chang HS,Park J,Cho JH,Park MR,Lee HJ,Han HJ

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Western Blot

Mon Mar 07 00:00:00 UTC 2022

Cyanidin 3-O-arabinoside suppresses DHT-induced dermal papilla cell senescence by modulating p38-dependent ER-mitochondria contacts.

Journal of biomedical science

Jung YH,Chae CW,Choi GE,Shin HC,Lim JR,Chang HS,Park J,Cho JH,Park MR,Lee HJ,Han HJ

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Western Blot

Mon Mar 07 00:00:00 UTC 2022

CUDC907, a dual phosphoinositide-3 kinase/histone deacetylase inhibitor, promotes apoptosis of NF2 Schwannoma cells.

Oncotarget

Huegel J,Dinh CT,Martinelli M,Bracho O,Rosario R,Hardin H,Estivill M,Griswold A,Gultekin S,Liu XZ,Fernandez-Valle C

MA5-14949 was used in Immunohistochemistry to screened PI3K pathway inhibitors for efficacy in reducing viability of human schwannoma cells.

Thu Aug 10 00:00:00 UTC 2023

Senolysis-Based Elimination of Chemotherapy-Induced Senescent Breast Cancer Cells by Quercetin Derivative with Blocked Hydroxy Groups.

Cancers

Lewińska A,Przybylski P,Adamczyk-Grochala J,Błoniarz D,Litwinienko G,Wnuk M

MA5-14949 was used in Immunocytochemistry-immunofluorescence to study targeted elimination of senescent cells using synthesized quercetin derivatives which were tested for improved senolytic action against etoposide-induced senescent human normal mammary epithelial cells and triple-negative breast cancer cells in vitro.

Tue Jan 25 00:00:00 UTC 2022

Decreased p53 is associated with a decline in asymmetric stem cell self-renewal in aged human epidermis.

Aging cell

Charruyer A,Weisenberger T,Li H,Khalifa A,Schroeder AW,Belzer A,Ghadially R

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Immunocytochemistry

Mon Feb 01 00:00:00 UTC 2021

Decreased p53 is associated with a decline in asymmetric stem cell self-renewal in aged human epidermis.

Aging cell

Charruyer A,Weisenberger T,Li H,Khalifa A,Schroeder AW,Belzer A,Ghadially R

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Immunocytochemistry

Mon Feb 01 00:00:00 UTC 2021

Interleukin-6 neutralization ameliorates symptoms in prematurely aged mice.

Aging cell

Squarzoni S,Schena E,Sabatelli P,Mattioli E,Capanni C,Cenni V,D'Apice MR,Andrenacci D,Sarli G,Pellegrino V,Festa A,Baruffaldi F,Storci G,Bonafè M,Barboni C,Sanapo M,Zaghini A,Lattanzi G

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Western Blot

Fri Jan 01 00:00:00 UTC 2021

Interleukin-6 neutralization ameliorates symptoms in prematurely aged mice.

Aging cell

Squarzoni S,Schena E,Sabatelli P,Mattioli E,Capanni C,Cenni V,D'Apice MR,Andrenacci D,Sarli G,Pellegrino V,Festa A,Baruffaldi F,Storci G,Bonafè M,Barboni C,Sanapo M,Zaghini A,Lattanzi G

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Western Blot

Fri Jan 01 00:00:00 UTC 2021

Insights into epithelial cell senescence from transcriptome and secretome analysis of human oral keratinocytes.

Aging

Schwartz RE,Shokhirev MN,Andrade LR,Gutkind JS,Iglesias-Bartolome R,Shadel GS

MA5-14949 was used in Immunocytochemistry-immunofluorescence to highlight senescence changes in epithelial cells and how they might contribute to chronic inflammation and age-related diseases.

Fri Feb 12 00:00:00 UTC 2021

Insights into epithelial cell senescence from transcriptome and secretome analysis of human oral keratinocytes.

Aging

Schwartz RE,Shokhirev MN,Andrade LR,Gutkind JS,Iglesias-Bartolome R,Shadel GS

MA5-14949 was used in Immunocytochemistry-immunofluorescence to highlight senescence changes in epithelial cells and how they might contribute to chronic inflammation and age-related diseases.

Fri Feb 12 00:00:00 UTC 2021

Baricitinib, a JAK-STAT Inhibitor, Reduces the Cellular Toxicity of the Farnesyltransferase Inhibitor Lonafarnib in Progeria Cells.

International journal of molecular sciences

Arnold R,Vehns E,Randl H,Djabali K

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Immunocytochemistry

Mon Jul 12 00:00:00 UTC 2021

Baricitinib, a JAK-STAT Inhibitor, Reduces the Cellular Toxicity of the Farnesyltransferase Inhibitor Lonafarnib in Progeria Cells.

International journal of molecular sciences

Arnold R,Vehns E,Randl H,Djabali K

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Immunocytochemistry

Mon Jul 12 00:00:00 UTC 2021

Palbociclib-mediated cell cycle arrest can occur in the absence of the CDK inhibitors p21 and p27.

Open biology

Pennycook BR,Barr AR

MA5-14949 was used in Immunocytochemistry and Western Blotting to clarify the mechanism of action of CDK4/6 inhibitors and has implications for cancer treatment and patient stratification.

Mon Nov 01 00:00:00 UTC 2021

Palbociclib-mediated cell cycle arrest can occur in the absence of the CDK inhibitors p21 and p27.

Open biology

Pennycook BR,Barr AR

MA5-14949 was used in Immunocytochemistry and Western Blotting to clarify the mechanism of action of CDK4/6 inhibitors and has implications for cancer treatment and patient stratification.

Mon Nov 01 00:00:00 UTC 2021

Palbociclib-mediated cell cycle arrest can occur in the absence of the CDK inhibitors p21 and p27.

Open biology

Pennycook BR,Barr AR

MA5-14949 was used in Immunocytochemistry and Western Blotting to clarify the mechanism of action of CDK4/6 inhibitors and has implications for cancer treatment and patient stratification.

Mon Nov 01 00:00:00 UTC 2021

Palbociclib-mediated cell cycle arrest can occur in the absence of the CDK inhibitors p21 and p27.

Open biology

Pennycook BR,Barr AR

MA5-14949 was used in Immunocytochemistry and Western Blotting to clarify the mechanism of action of CDK4/6 inhibitors and has implications for cancer treatment and patient stratification.

Mon Nov 01 00:00:00 UTC 2021

The lack of functional DNMT2/TRDMT1 gene modulates cancer cell responses during drug-induced senescence.

Aging

Bloniarz D,Adamczyk-Grochala J,Lewinska A,Wnuk M

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Immunocytochemistry

Thu Jun 17 00:00:00 UTC 2021

AMPK-mediated senolytic and senostatic activity of quercetin surface functionalized Fe₃O₄ nanoparticles during oxidant-induced senescence in human fibroblasts.

Redox biology

Lewinska A,Adamczyk-Grochala J,Bloniarz D,Olszowka J,Kulpa-Greszta M,Litwinienko G,Tomaszewska A,Wnuk M,Pazik R

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Immunofluorescence

Wed Jan 01 00:00:00 UTC 2020

AMPK-mediated senolytic and senostatic activity of quercetin surface functionalized Fe₃O₄ nanoparticles during oxidant-induced senescence in human fibroblasts.

Redox biology

Lewinska A,Adamczyk-Grochala J,Bloniarz D,Olszowka J,Kulpa-Greszta M,Litwinienko G,Tomaszewska A,Wnuk M,Pazik R

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Immunofluorescence

Wed Jan 01 00:00:00 UTC 2020

AMPK-mediated senolytic and senostatic activity of quercetin surface functionalized Fe₃O₄ nanoparticles during oxidant-induced senescence in human fibroblasts.

Redox biology

Lewinska A,Adamczyk-Grochala J,Bloniarz D,Olszowka J,Kulpa-Greszta M,Litwinienko G,Tomaszewska A,Wnuk M,Pazik R

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Immunofluorescence

Wed Jan 01 00:00:00 UTC 2020

Emerin Phosphorylation during the Early Phase of the Oxidative Stress Response Influences Emerin-BAF Interaction and BAF Nuclear Localization.

Cells

Cenni V,Squarzoni S,Loi M,Mattioli E,Lattanzi G,Capanni C

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Western Blot

Sat Jun 06 00:00:00 UTC 2020

Emerin Phosphorylation during the Early Phase of the Oxidative Stress Response Influences Emerin-BAF Interaction and BAF Nuclear Localization.

Cells

Cenni V,Squarzoni S,Loi M,Mattioli E,Lattanzi G,Capanni C

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Western Blot

Sat Jun 06 00:00:00 UTC 2020

Sodium butyrate inhibits high cholesterol-induced neuronal amyloidogenesis by modulating NRF2 stabilization-mediated ROS levels: involvement of NOX2 and SOD1.

Cell death & disease

Kim SY,Chae CW,Lee HJ,Jung YH,Choi GE,Kim JS,Lim JR,Lee JE,Cho JH,Park H,Park C,Han HJ

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Western Blot

Thu Jun 18 00:00:00 UTC 2020

Modulated Electro-Hyperthermia-Induced Tumor Damage Mechanisms Revealed in Cancer Models.

International journal of molecular sciences

Krenacs T,Meggyeshazi N,Forika G,Kiss E,Hamar P,Szekely T,Vancsik T

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Immunocytochemistry

Sat Aug 29 00:00:00 UTC 2020

Modulated Electro-Hyperthermia-Induced Tumor Damage Mechanisms Revealed in Cancer Models.

International journal of molecular sciences

Krenacs T,Meggyeshazi N,Forika G,Kiss E,Hamar P,Szekely T,Vancsik T

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Immunocytochemistry

Sat Aug 29 00:00:00 UTC 2020

Inhibition of JAK-STAT Signaling with Baricitinib Reduces Inflammation and Improves Cellular Homeostasis in Progeria Cells.

Cells

Liu C,Arnold R,Henriques G,Djabali K

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Western Blot

Fri Oct 18 00:00:00 UTC 2019

Inhibition of JAK-STAT Signaling with Baricitinib Reduces Inflammation and Improves Cellular Homeostasis in Progeria Cells.

Cells

Liu C,Arnold R,Henriques G,Djabali K

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Western Blot

Fri Oct 18 00:00:00 UTC 2019

Snake venoms promote stress-induced senescence in human fibroblasts.

Journal of cellular physiology

Lewinska A,Bocian A,Petrilla V,Adamczyk-Grochala J,Szymura K,Hendzel W,Kaleniuk E,Hus KK,Petrillova M,Wnuk M

MA5-14949 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to investigate snake-venom-induced adaptive response at a relatively noncytotoxic concentration (0.01 µg/ml) in two human fibroblast cell lines of different origin, namely WI-38 fetal lung fibroblasts and BJ foreskin fibroblasts.

Wed May 01 00:00:00 UTC 2019

Altered modulation of lamin A/C-HDAC2 interaction and p21 expression during oxidative stress response in HGPS.

Aging cell

Mattioli E,Andrenacci D,Garofalo C,Prencipe S,Scotlandi K,Remondini D,Gentilini D,Di Blasio AM,Valente S,Scarano E,Cicchilitti L,Piaggio G,Mai A,Lattanzi G

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Western Blot

Mon Oct 01 00:00:00 UTC 2018

Altered modulation of lamin A/C-HDAC2 interaction and p21 expression during oxidative stress response in HGPS.

Aging cell

Mattioli E,Andrenacci D,Garofalo C,Prencipe S,Scotlandi K,Remondini D,Gentilini D,Di Blasio AM,Valente S,Scarano E,Cicchilitti L,Piaggio G,Mai A,Lattanzi G

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Western Blot

Mon Oct 01 00:00:00 UTC 2018

Altered modulation of lamin A/C-HDAC2 interaction and p21 expression during oxidative stress response in HGPS.

Aging cell

Mattioli E,Andrenacci D,Garofalo C,Prencipe S,Scotlandi K,Remondini D,Gentilini D,Di Blasio AM,Valente S,Scarano E,Cicchilitti L,Piaggio G,Mai A,Lattanzi G

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Western Blot

Mon Oct 01 00:00:00 UTC 2018

Altered modulation of lamin A/C-HDAC2 interaction and p21 expression during oxidative stress response in HGPS.

Aging cell

Mattioli E,Andrenacci D,Garofalo C,Prencipe S,Scotlandi K,Remondini D,Gentilini D,Di Blasio AM,Valente S,Scarano E,Cicchilitti L,Piaggio G,Mai A,Lattanzi G

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Western Blot

Mon Oct 01 00:00:00 UTC 2018

Reduced levels of methyltransferase DNMT2 sensitize human fibroblasts to oxidative stress and DNA damage that is accompanied by changes in proliferation-related miRNA expression.

Redox biology

Lewinska A,Adamczyk-Grochala J,Kwasniewicz E,Deregowska A,Semik E,Zabek T,Wnuk M

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Western Blot

Sun Apr 01 00:00:00 UTC 2018

Preclinical Efficacy of the MDM2 Inhibitor RG7112 in MDM2-Amplified and TP53 Wild-type Glioblastomas.

Clinical cancer research : an official journal of the American Association for Cancer Research

Verreault M,Schmitt C,Goldwirt L,Pelton K,Haidar S,Levasseur C,Guehennec J,Knoff D,Labussière M,Marie Y,Ligon AH,Mokhtari K,Hoang-Xuan K,Sanson M,Alexander BM,Wen PY,Delattre JY,Ligon KL,Idbaih A

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Immunocytochemistry

Tue Mar 01 00:00:00 UTC 2016

Preclinical Efficacy of the MDM2 Inhibitor RG7112 in MDM2-Amplified and TP53 Wild-type Glioblastomas.

Clinical cancer research : an official journal of the American Association for Cancer Research

Verreault M,Schmitt C,Goldwirt L,Pelton K,Haidar S,Levasseur C,Guehennec J,Knoff D,Labussière M,Marie Y,Ligon AH,Mokhtari K,Hoang-Xuan K,Sanson M,Alexander BM,Wen PY,Delattre JY,Ligon KL,Idbaih A

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Immunocytochemistry

Tue Mar 01 00:00:00 UTC 2016

Disentangling the aneuploidy and senescence paradoxes: a study of triploid breast cancers non-responsive to neoadjuvant therapy.

Histochemistry and cell biology

Gerashchenko BI,Salmina K,Eglitis J,Huna A,Grjunberga V,Erenpreisa J

MA5-14949 was used in immunohistochemistry to analyze triploid breast cancers non-responsive to neoadjuvant therapy by study of aneuploidy and senescence paradoxes

Fri Apr 01 00:00:00 UTC 2016

Detection of mesenchymal stem cells senescence by prelamin A accumulation at the nuclear level.

SpringerPlus

Bellotti C,Capanni C,Lattanzi G,Donati D,Lucarelli E,Duchi S

MA514949 was used in immunocytochemistry to correlate lamin A maturation and cellular senescence

Wed Sep 14 00:00:00 UTC 2016

Role of stress-activated OCT4A in the cell fate decisions of embryonal carcinoma cells treated with etoposide.

Cell cycle (Georgetown, Tex.)

Huna A,Salmina K,Erenpreisa J,Vazquez-Martin A,Krigerts J,Inashkina I,Gerashchenko BI,Townsend PA,Cragg MS,Jackson TR

MA5-14949 was used in western blot to elucidate the mechanism of p53-dependent upregulation of OCT4A and p21Cip1.

Wed Aug 03 00:00:00 UTC 2016

Sp1 acetylation is associated with loss of DNA binding at promoters associated with cell cycle arrest and cell death in a colon cell line.

Molecular cancer

Waby JS,Chirakkal H,Yu C,Griffiths GJ,Benson RS,Bingle CD,Corfe BM

Published figure using p21 monoclonal antibody (Product # MA5-14949) in Immunocytochemistry

Fri Oct 15 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: Activating Fragment 1; CDK-interacting protein 1; CDK-interaction protein 1; CDNK1A; Cip1); Cyclin-dependent kinase inhibitor 1; Cyclin-dependent kinase inhibitor 1A (p21; cyclin-dependent kinase inhibitor 1A (p21, Cip1); DNA synthesis inhibitor; MDA-6; melanoma differentiation associated protein 6; Melanoma differentiation-associated protein 6; OTTHUMP00000016298; p21; p21Cip1/Waf1; SLC12A9; Wild type p53 activated fragment 1 (WAF1); wild-type p53-activated fragment 1

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Gene Aliases: CAP20; CDKN1; CDKN1A; CIP1; MDA-6; MDA6; P21; p21CIP1; PIC1; SDI1; WAF1

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UniProt ID: (Human) P38936

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Entrez Gene ID: (Human) 1026

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Function(s)

cyclin-dependent protein serine/threonine kinase inhibitor activity protein binding cyclin-dependent protein kinase activating kinase activity cyclin binding ubiquitin protein ligase binding protein complex binding metal ion binding protein-binding activity modulator kinase inhibitor kinase modulator

Process(es)

regulation of cyclin-dependent protein serine/threonine kinase activity G1/S transition of mitotic cell cycle G2/M transition of mitotic cell cycle cellular response to DNA damage stimulus DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest cell cycle arrest Ras protein signal transduction negative regulation of cell proliferation response to toxic substance response to X-ray response to organonitrogen compound negative regulation of gene expression negative regulation of cell growth positive regulation of B cell proliferation organ regeneration cellular response to extracellular stimulus regulation of protein import into nucleus, translocation cellular response to amino acid starvation cellular response to heat negative regulation of phosphorylation response to drug intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator negative regulation of apoptotic process positive regulation of programmed cell death negative regulation of cyclin-dependent protein serine/threonine kinase activity response to arsenic-containing substance positive regulation of fibroblast proliferation protein stabilization response to corticosterone response to hyperoxia intestinal epithelial cell maturation cellular response to ionizing radiation cellular response to UV-B mitotic cell cycle arrest cellular senescence replicative senescence stress-induced premature senescence intrinsic apoptotic signaling pathway positive regulation of cyclin-dependent protein kinase activity negative regulation of G1/S transition of mitotic cell cycle regulation of DNA biosynthetic process positive regulation of reactive oxygen species metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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p21 Monoclonal Antibody (R.229.6)

p21 Antibody (MA5-14949) in ICC/IF

Product Details

MA5-14949

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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