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Invitrogen
p35 Polyclonal Antibody
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FIGURE: 1 / 4
p35 Antibody (PA5-64865) in ICC/IF
Immunofluorescence analysis of p35 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with p35 Polyclonal Antibody (Product # PA5-64865) at 5 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034... View More
Product Details
PA5-64865
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human p35. Recombinant protein control fragment (Product #RP-105673).
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: FITPANVVFLY MLCRDVISSE VGSDHELQAV LLTCLYLSYS YMGNEISYPL KPFLVESCKE AFWDRCLSVI NLMSSKMLQI NADPHYFTQV FSDLKNESGQ EDK
Highest antigen sequence identity to the following orthologs - mouse 100%, rat 100%.
Target Information
The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDK5 activator 1; Cyclin-dependent kinase 5 activator 1; Cyclin-dependent kinase 5 regulatory subunit 1; cyclin-dependent kinase 5, regulatory subunit 1 (p35); neuronal CDK5 activator; regulatory partner for CDK5 kinase; tau protein kinase II 23kDa subunit; TPKII regulatory subunit
Gene Aliases: CDK5P35; CDK5R; CDK5R1; NCK5A; p23; p25; p35; p35nck5a
UniProt ID: (Human) Q15078
Entrez Gene ID: (Human) 8851
protein kinase activity
cyclin-dependent protein serine/threonine kinase activity
calcium ion binding
protein binding
kinase activity
cyclin-dependent protein kinase 5 activator activity
protein kinase binding
protein serine/threonine kinase activator activity
cadherin binding
ephrin receptor binding
kinase activator
regulation of cyclin-dependent protein serine/threonine kinase activity
neuron migration
neuron cell-cell adhesion
G-protein coupled acetylcholine receptor signaling pathway
axon guidance
axonal fasciculation
brain development
cell proliferation
embryo development
regulation of macroautophagy
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
cerebellum development
superior olivary nucleus maturation
hippocampus development
layer formation in cerebral cortex
neuron differentiation
negative regulation of axon extension
neuron projection development
regulation of actin cytoskeleton organization
serine phosphorylation of STAT3 protein
ionotropic glutamate receptor signaling pathway
positive regulation of neuron apoptotic process
regulation of neuron differentiation
negative regulation of transcription, DNA-templated
ephrin receptor signaling pathway
rhythmic process
regulation of dendritic spine morphogenesis
regulation of microtubule cytoskeleton organization
positive regulation of cell cycle arrest
positive regulation of protein serine/threonine kinase activity
positive regulation of protein targeting to membrane
regulation of signal transduction by p53 class mediator
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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