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Invitrogen
p47phox Polyclonal Antibody
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FIGURE: 1 / 5
p47phox Antibody (PA5-17225) in ICC/IF
Immunofluorescence analysis of p47phox was performed using 70% confluent log phase Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with p47phox Rabbit Polyclonal Antibody (Product # PA5-17225) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product #... View More
Product Details
PA5-17225
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues surrounding Arg292 of human p47phox
Conjugate
Form
Liquid
Concentration
105 µg/mL
Amount
10.5 µg
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
p47phox (NCF1) is required, along with NCF2 and membrane bound cytochrome b558, for activation of the latent NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in the gene can result in granulomatous disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NADPH oxidase organizer 2; NCF-1; NCF-47K; neutrop; Neutrophil cytosol factor 1; neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1); Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; P47 phox; p47-phox; SH3 and PX domain-containing protein 1A; unnamed protein product
Gene Aliases: CGD1; NCF-1; NCF-47K; NCF1; NCF1A; NOXO2; p47-phox; p47phox; SH3PXD1A
UniProt ID: (Human) P14598
Entrez Gene ID: (Human) 653361
response to yeast
leukocyte mediated cytotoxicity
respiratory burst involved in defense response
protein targeting to membrane
leukotriene metabolic process
superoxide metabolic process
inflammatory response
cellular defense response
response to bacterium
superoxide anion generation
defense response to bacterium
innate immune response
respiratory burst
hydrogen peroxide biosynthetic process
epithelial cell proliferation
defense response to Gram-positive bacterium
defense response to fungus
regulation of respiratory burst involved in inflammatory response
neutrophil mediated killing of gram-positive bacterium
neutrophil mediated killing of fungus
cellular response to glucose stimulus
cellular response to testosterone stimulus
reactive oxygen species metabolic process
reactive oxygen species biosynthetic process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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