Immunofluorescent analysis of p53 showing staining in the nucleus of HCT 116 cells. HCT 116 cells mock (left) and treated with 30µM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min and stained using a p53 polyclonal antibody (Product # PA5-27822) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 142 and 393 of Human p53|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27822 targets p53 in IF, IHC (P), IP, ChIP and WB applications and shows reactivity with Human samples.
The PA5-27822 immunogen is recombinant fragment corresponding to a region within amino acids 142 and 393 of Human p53.
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons
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