Proteintech

p70(S6K) Polyclonal Antibody

Product Image Gallery

p70(S6K) Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of HepG2 cells using 14485-1-AP (p70S6K antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.

p70(S6K) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14485-1-AP ( p70S6K antibody at dilution of 1:200 (under 40x lens).

p70(S6K) Antibody in Western Blot (WB) — Rat spleen tissue were subjected to SDS PAGE followed by western blot with 14485-1-AP (p70S6K antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

p70(S6K) Antibody in Flow Cytometry (Flow) — 1X10^6 HepG2 cells were stained with 0.2ug p70S6K antibody (14485-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

p70(S6K) Antibody in Immunoprecipitation (IP) — IP result of anti-p70S6K (IP:14485-1-AP, 3ug; Detection:14485-1-AP 1:1000) with HeLa cells lysate 3000ug.

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunocytochemistry (ICC)

1:10-1:100

Immunofluorescence (IF)

1:10-1:100

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunoprecipitation (IP)

0.5-4.0 µg

Western Blot (WB)

1:500-1:2400

View 32 publications 32 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Fish, Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

p70(S6K) Fusion Protein Ag5883

Conjugate

Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

The p70 S6 kinase (p70 Ribosomal Protein S6 Kinase, p70S6K) is a 70 kDa member of the ribosomal S6 kinase (RSK) family of serine/threonine kinases. p70 S6 kinase is predominantly localized in the cytoplasm, and is essential in growth factors regulated cell proliferation, pathways involving cell motility, such as metastases, the immune response, and tissue repair. p70 S6 kinase acts downstream of phosphoinositide (PI) 3-kinase, and its main physiological target is the S6 ribosomal protein, which is involved in upregulation of protein synthesis. The kinase activity of p70 S6 kinase leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding p70 S6 kinase and overexpression are seen in some breast cancer cell lines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 70 kDa ribosomal protein S6 kinase 1; EC 2.7.11.1; kinase p70S6K; KS6B1; p70 p70-S6K; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6 kinase, alpha; p70 S6K-alpha; p70 S6KA; p70(S6K)-alpha; p70-S6K 1; P70S6K; p70S6K-alpha; P70S6K1; p80-S6K 1; p85 p85S6K; Ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase I; ribosomal protein S6 kinase, 70kD, polypeptide 1; ribosomal protein S6 kinase, 70kDa, polypeptide 1; S6 kinase; S6K; S6K-beta-1; S6K1; serine/threonine kinase 14 alpha; Serine/threonine-protein kinase 14A

Gene Aliases: 2610318I15Rik; 4732464A07Rik; 70kDa; AA959758; AI256796; AI314060; p70 S6KA; p70(S6K)-alpha; p70-alpha; p70-S6K; p70/85s6k; p70s6k; PS6K; RPS6KB1; S6K; S6K-beta-1; S6K1; STK14A

UniProt ID: (Human) P23443, (Mouse) Q8BSK8, (Rat) P67999

Entrez Gene ID: (Human) 6198, (Mouse) 72508, (Rat) 83840

Molecular Function: annexin calcium-binding protein calmodulin intracellular calcium-sensing protein kinase non-receptor serine/threonine protein kinase protein kinase transfer/carrier protein transferase