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Agrisera
pHRed Polyclonal Antibody
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Product Details
AS16-3828
Product Specifications
Species Reactivity
Tag
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant part of pHRed of Chlamydomonas reinhardtii.
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: pHRed
Target Information
pHRed is a genetically encoded, cytosolically expressed fluorescent protein sensor engineered from the monomeric red fluorescent protein mKeima and is typically introduced into mammalian cells as a synthetic coding sequence for live-cell imaging; when expressed without targeting motifs it reports bulk intracellular (cytosolic) pH, and when fused to organelle-localization sequences it can be used to measure compartment-specific pH (e.g., mitochondria). Structurally, pHRed is a single polypeptide fluorescent protein that folds into the characteristic beta-barrel (GFP-like) architecture surrounding an autocatalytically formed chromophore, and it behaves as a ratiometric indicator with two excitation maxima (approximately 440 and 585 nm) and red emission (approximately 610 nm). Functionally, pHRed transduces changes in protonation state into optical readouts: the excitation ratio changes with pH with an apparent pKa near 6.6 and a large dynamic range, enabling quantitative monitoring of physiologic pH shifts in living cells; in addition to intensity-based ratiometry, pHRed exhibits a pH-dependent fluorescence lifetime that can be exploited for lifetime imaging approaches to track energy-dependent pH changes in the cytosol or mitochondria.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: pHRed; pHRed biosensor
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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