Immunofluorescent analysis of Ras (green) in U20S cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product #31873) for 15 minutes at room temperature. Cells were then probed with a mouse monoclonal antibody recognizing Ras (Product# MA1-012), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
|Tested species reactivity||Human, Mouse, Non-human primate|
|Published species reactivity||Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Purified recombinant Ras protein|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay dependent|
|Immunofluorescence (IF)||1:50 - 1:200|
|Immunoprecipitation (IP)||2 µg|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-012 has been successfully used in immunoprecipitation, immunohistochemistry, flow cytometry, immunofluorescence and Western Blot applications on human, mouse and monkey samples.
This antibody recognizes normal p21, activated p21, and p21 from each of the Harvey, Kirsten and N-ras families.
This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.
IP-MS enrichment of (LFQ intensity): KRAS AND HRAS were enriched 1895-fold and 255-fold, respectively, from HCT116 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and pan RAS antibody (Part No. MA1-012 ). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Inhibition of RAS activation due to a homozygous ezrin variant in patients with profound intellectual disability.
MA1-012 was used in western blot to report that ezrin is required for Ras activation.
|Riecken LB,Tawamie H,Dornblut C,Buchert R,Ismayel A,Schulz A,Schumacher J,Sticht H,Pohl KJ,Cui Y,Reis A,Morrison H,Abou Jamra R||Human mutation (36:270)||2015|
|Not Applicable||Not Cited||
An improved isoprenylcysteine carboxylmethyltransferase inhibitor induces cancer cell death and attenuates tumor growth in vivo.
MA1-012 was used in western blot to show that 8.12 is a pharmacological inhibitor of Icmt
|Lau HY,Ramanujulu PM,Guo D,Yang T,Wirawan M,Casey PJ,Go ML,Wang M||Cancer biology and therapy (15:1280)||2014|
Production and characterization of anti-RAS p21 monoclonal antibodies.
MA1-012 was used in [flow cytometry] to characterize the antibody's reactivity to normal p21, activated p21, and p21 from each of the Harvey, Kirsten and N-ras families.
|Hamer PJ,Trimpe KL,Pullano T,Ng S,LaVecchio JA,Petit DA,DeLellis R,Wolfe H,Carney WP||Hybridoma (9:573)||1990|