|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide near N-terminus of human alpha, beta, lambda synuclein protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody is predicted to react with rat samples based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is brain tissue.
Synucleins are small, soluble proteins expressed primarily in neural tissue and in certain tumors. The family includes three known proteins: alpha-synuclein, beta-synuclein, and gamma-synuclein. The alpha- and beta-synuclein proteins are found primarily in brain tissue, where they are seen mainly in presynaptic terminals. The gamma-synuclein protein is found primarily in the peripheral nervous system and retina, but its expression in breast tumors is a marker for tumor progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
First appraisal of brain pathology owing to A30P mutant alpha-synuclein.
PA1-38705 was used in western blot to investigate the involvement of A30P mutant alpha-synuclein on familial parkinson disease
|Seidel K,Schöls L,Nuber S,Petrasch-Parwez E,Gierga K,Wszolek Z,Dickson D,Gai WP,Bornemann A,Riess O,Rami A,Den Dunnen WF,Deller T,Rüb U,Krüger R||Annals of neurology (67:684)||2010|